p27BBP/eIF6 acts as an anti-apoptotic factor upstream of Bcl-2 during Xenopus laevis development

Marco, Nadia De; Iannone, Lucio; Carotenuto, Rosa; Biffo, Stefano; Vitale, Aniello; Campanella, Chiara

doi:10.1038/cdd.2009.128

Cited by 26 publications

(38 citation statements)

References 34 publications

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“…32 Thus, data suggest that eIF6 is the first 60S-associated factor able to modulate translation downstream of extracellular signaling in vivo. Polysomal profile from cells with different eIF6 levels and conditions have different features confirming the pleiotropic role of eIF6 on 60S ( Table 1).…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 99%

Translational control by 80S formation and 60S availability: The central role of eIF6, a rate limiting factor in cell cycle progression and tumorigenesis

Brina¹,

Grosso²,

Miluzio³

et al. 2011

Cell Cycle

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Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tmentioning

confidence: 99%

Translational control by 80S formation and 60S availability: The central role of eIF6, a rate limiting factor in cell cycle progression and tumorigenesis

Brina¹,

Grosso²,

Miluzio³

et al. 2011

Cell Cycle

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“…The repartition of p27BBP/eIF6, a protein the decrease of which was accompanied by a reduction in proliferation of several cells in eIF6 mice, was studied throughout the development of Xenopus [41]. At the beginning of embryogenesis, a correspondence between highest levels of p27BBP/eIF6, cell proliferation and apoptosis was observed.…”

Section: Regulation Of Apoptosis At Neurulationmentioning

confidence: 99%

“…In later development stages high proliferation levels were present in the same regions where higher p27BBP/eIF6 expression was observed, while apoptosis were not concentrated in the same sites. So, a high presence of p27BBP/eIF6 would appear in the regions where cell death is essential for normal development [41].…”

Section: Regulation Of Apoptosis At Neurulationmentioning

confidence: 99%

Apoptosis in amphibian development

Exbrayat¹,

Moudilou²,

Abrouk³

et al. 2012

ABB

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Amphibians and more particularly X. laevis are models often used for studying apoptosis during embryonic development. Using several methods, searchers determined the localization of programmed cell deaths (PCD). Several experimental methods also have been used to understand the regulatory mechanisms of apoptosis, throughout development, contributing to elucidate the general action of several genes and proteins. Apoptosis occurs very early, with a first program under control of maternal genes expressed before MBT, in order to eliminate damaged cells before gastrulation, and a second program at the onset of gastrulation. PCD is also observed during neurulation. Then, apoptotic cells are observed in amphibian organogenesis and metamorphosis. Results of these researches showed both importance of PCD for embryonic development, and the complexity of its regulation. Results obtained can be useful to understand others aspects of the importance of apoptosis, particularly pathological aspects.

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“…et De Marco et al, 2010), the study of this protein in embryogenesis is quite appealing because it may help to explain steps of anlagen determination or differentiation.…”

Section: Abstract: Pronephros Xenopus Laevis Eukaryotic Initiationmentioning

confidence: 99%

“…Eif6-60S is probably part of the RISC (RNA-induced silencing complex) and as such may regulate the availability of mRNAs for translation (Chendrimada et al, 2007). Because EIF6 appears to act selectively on specific mRNAs (Ji Abbreviations used in this paper: Akt, serine/threonine-protein kinase; bgal, bgalactosidase; Clcnk , chloride channel Kb; cdh16 , human cadherin-16; Eif6 1 , eukaryotic initiation factor 6; IGF, insulin growth factor; Igfr, insulin growth factor receptor; gipc2, GAIP interacting protein, C terminus; lhx1, LIM homeobox 1; miR, microRNA; myod1, myogenic differentiation 1; nphs1, nephrin; p110*, protein 110 (subunit of the PI3 kinase); pi3/kinase, phosphatidylinositol 3-kinase; RISC, RNA-induced silencing complex; S235A, eif6 mutated in serine 235; slc5a9, solute carrier family 5 (sodium/glucose cotransporter), member 9.et De Marco et al, 2010), the study of this protein in embryogenesis is quite appealing because it may help to explain steps of anlagen determination or differentiation.We previously showed that, in Xenopus laevis, eif6 is a factor regulating apoptosis upstream of bcl-2/bax (De Marco et al, 2010). Moreover, eif6 over-expression causes a delay in eye develop-…”

mentioning

confidence: 99%

Involvement of the eukaryotic initiation factor 6 and kermit2/gipc2 in Xenopus laevis pronephros formation

Tussellino¹,

Marco²,

Campanella³

et al. 2012

Int. J. Dev. Biol.

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The translation initiation factor Eif6 has been implicated as a regulator of ribosome assembly, selective mRNA translation and apoptosis. Many of these activities depend upon the phosphorylation of eif6 Serine 235 by protein kinase C (PKC). Eif6-60S is probably part of the RNAinduced silencing complex (RISC). eif6 over-expression in Xenopus embryos causes aberrant eye development. kermit2/gipc2 morphants have an eye phenotype similar to that of the eif6 overexpressors. Eye formation is regulated by insulin growth factor (IGF) signalling. eif6 interacts with the IGF receptor (IGFR) and kermit2/gipc2, which also binds to igfr. eif6 over-expression in Xenopus causes also the formation of antero-ventral oedema, suggesting a malfunction of the excretory system. Here we evaluated the pronephros phenotype. The oedema grows into the nephrocoel, expanding its boundary and is accompanied by a strong reduction of the pronephros. The three main components of the pronephros are severely impaired in eif6 over-expressors, while are not affected in eif6 morphants. Conversely, gipc2 depletion induces the oedema phenotype and reduction of the pronephros, while gipc2 overexpression does not. p110*, a constitutively active p110 subunit of the PI3 kinase partially recovers the oedema phenotype. We also determined that PKC-dependent phosphorylation of Ser235 in eif6 is not required to produce defective pronephroi. These results indicate that the levels of eif6 are highly regulated during development and instrumental for proper morphogenesis of the pronephros. Moreover, it appears that for proper pronephros development the gipc2 level should be kept within or over the physiological range and that the oedema phenotype is partly due to the inhibition of IGF signalling. KEY WORDS: pronephros, Xenopus laevis, eukaryotic initiation factor 6, kermit2/gipc2Eif6 1 (eukaryotic initiation factor 6) is a protein essential for cell survival that plays important roles in ribosome biogenesis and translation (see Ceci et al., 2003). In particular, it is associated with the plasma membrane where it interacts with b4 integrin as well as the cytoskeleton (Biffo et al., 1997). Eif6 can be regulated by extracellular signals, such as IGF (Gandin et al., 2008), and is itself a regulator of translation at the 60S ribosomal subunit. When unphosphorylated, it impedes the joining of the 60S with the 40S ribosomal subunit. When phosphorylated by PKC, Eif6 separates from the 60S subunit. As a consequence, ribosomal subunits 60S and 40S may join, allowing mRNA translation to occur (Ceci et al., 2003). Eif6-60S is probably part of the RISC (RNA-induced silencing complex) and as such may regulate the availability of mRNAs for translation (Chendrimada et al., 2007). Because EIF6 appears to act selectively on specific mRNAs (Ji Abbreviations used in this paper: Akt, serine/threonine-protein kinase; bgal, bgalactosidase; Clcnk , chloride channel Kb; cdh16 , human cadherin-16; Eif6 1 , eukaryotic initiation factor 6; IGF, insulin growth factor; Igfr, insulin g...

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p27BBP/eIF6 acts as an anti-apoptotic factor upstream of Bcl-2 during Xenopus laevis development

Cited by 26 publications

References 34 publications

Translational control by 80S formation and 60S availability: The central role of eIF6, a rate limiting factor in cell cycle progression and tumorigenesis

Translational control by 80S formation and 60S availability: The central role of eIF6, a rate limiting factor in cell cycle progression and tumorigenesis

Apoptosis in amphibian development

Involvement of the eukaryotic initiation factor 6 and kermit2/gipc2 in Xenopus laevis pronephros formation

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