P2X (Purinergic) receptor distributions in rat blood vessels

Hansen, Mitchell A.; Dutton, Julie L.; Balcar, Vladimír J.; Barden, Julian A.; Bennett, Maxwell R.

doi:10.1016/s0165-1838(98)00189-1

Cited by 96 publications

(56 citation statements)

References 46 publications

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“…Nori et al [33] observed the coexpression of mRNAs of three P2X receptor subtypes (P2X 1 , P2X 2 , and P2X 4 ) in rat vascular smooth muscle. Some previous studies found that P2X 4 subtype did not couple to a vasomotor response [34] and that P2X 2 receptor was mainly located on nerves and arterial endothelial cells, but with low density on the smooth muscle cells [35]. α,β-MeATP was inactive as an agonist at the recombinant P2X 2 receptor [36].…”

Section: Discussionmentioning

confidence: 91%

Physiological significance of P2X receptor-mediated vasoconstriction in five different types of arteries in rats

Jia²,

Chen

et al. 2011

Purinergic Signalling

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P2X 1 receptors, the major subtype of P2X receptors in the vascular smooth muscle, are essential for α,β-methylene adenosine 5′-triphosphate (α,β-MeATP)-induced vasoconstriction. However, relative physiological significance of P2X 1 receptor-regulated vasoconstriction in the different types of arteries in the rat is not clear as compared with α 1 -adrenoceptor-regulated vasoconstriction. In the present study, we found that vasoconstrictive responses to noncumulative administration of α,β-MeATP in the rat isolated mesenteric arteries were significantly smaller than those to single concentration administration of α,β-MeATP. Therefore, we firstly reported the characteristic of α,β-MeATP-regulated vasoconstrictions in rat tail, internal carotid, pulmonary, mesenteric arteries, and aorta using single concentration administration of α,β-MeATP. The rank order of maximal vasoconstrictions for α,β-MeATP (E max·α,β-MeATP ) was the same as that of maximal vasoconstrictions for noradrenaline (E max·NA ) in the internal carotid, pulmonary, mesenteric arteries, and aorta. Moreover, the value of (E max·α,β-MeATP /E max·KCl )/(E max·NA /E max·KCl ) was 0.4 in each of the four arteries, but it was 0.8 in the tail artery. In conclusion, P2X 1 receptor-mediated vasoconstrictions are equally important in rat internal carotid, pulmonary, mesenteric arteries, and aorta, but much greater in the tail artery, suggesting its special role in physiological function.

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Section: Discussionmentioning

confidence: 91%

Physiological significance of P2X receptor-mediated vasoconstriction in five different types of arteries in rats

Jia²,

Chen

et al. 2011

Purinergic Signalling

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“…Previously we have described the presence of P2X $ receptors on vascular endothelial cells in the thymus by lightmicroscopy (Glass et al 2000) and of P2X # receptors on endothelial cells by electron-microscopy (Loesch & Burnstock, 2000). Confocal microscopy has shown an abundance of P2X # receptors on endothelial cells (Hansen et al 1999).…”

Section: P2x Receptors On Endothelial Cellsmentioning

confidence: 94%

Immunohistochemical identification of cells expressing ATP‐gated cation channels (P2X receptors) in the adult rat thyroid

Glaß

Burnstock

2001

Journal of Anatomy

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We carried out immunohistochemistry and western blotting of fresh frozen sections and crude extracts from adult rat thyroids. The histochemical and immunoblotting studies were performed with P2X receptor antibodies from 2 different sources. P2X-immunopositive cells were identified by fluorescence double labelling and confocal microscopy. Results of the western blotting experiments showed double bands of approximately 70 kDa and 140 kDa for all 7 P2X receptor subtypes with both sets of antibodies. Histochemical stains with antibodies from both sources also gave essentially identical results. P2X " , P2X # and P2X' receptors were detected exclusively in vascular smooth muscle ; P2X & and P2X ( receptors were also present on vascular smooth muscle. Endothelial cells stained for P2X $ , P2X % and P2X ( receptors. Thyroid follicular cells displayed immunoreactivity for P2X $ , P2X % and P2X & receptors. No immunostaining for P2X receptors was observed on C-cells. Possible roles for the broad expression of P2X receptor subtypes in the rat thyroid are discussed.Key words : Western blotting ; rat ; immunohistochemistry. Extracellular nucleotides have been shown to play an important role in cell signalling of excitable and nonexcitable cells (Abbracchio & Burnstock, 1998). Nucleotides are recognised as neurotransmitters of the central and the peripheral nervous systems (Burnstock, 1997(Burnstock, , 1999, and have been shown to be messengers in cellular growth and differentiation (Abbracchio, 1996) and to mediate apoptosis (Chow et al. 1997).Two families of receptors for extracellular nucleotides have been cloned. P2Y receptors are G proteincoupled receptors responding to purine and pyrimidine nucleotides, whereas P2X receptor are ATPgated, nonselective cation channels (Abbraccchio & Burnstock, 1994 ;Ralevic & Burnstock, 1998). The family of P2X receptors can be further subdivided into 7 subtypes, named P2X " to P2X ( (Burnstock & King, 1996 ;North & Surprenant, 2000). These P2X receptor subtypes seem to assemble to homomeric and Correspondence to Professor G. Burnstock, Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK. Tel. : j44 20 7830 2948 ; fax : j44 20 7830 2949 ; e-mail : g.burnstock!ucl.ac.uk heteromeric oligomers, which form the functional channel (Torres et al. 1999). The different P2X receptor homomers and heteromers were shown to have different pharmacological properties. The ability of P2X receptors to assemble to oligomers and the paucity of selective agonists and antagonists for P2X receptors make the pharmacological identification difficult. To identify the expression of P2X receptors in native tissues and cell cultures specific antibodies for all 7 P2X receptors subtypes have been generated (Oglesby et al. 1999) and recently antibodies for P2X receptors from commercial sources have also become available.In the present study we investigate the expression of P2X receptors in thyroid glands from adult rats. Th...

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“…In blood vessels, the nerves are confined to the adventitial side of the media muscle coat, and this geometry appears to facilitate dual control of vascular smooth muscle by perivascular nerves and by endothelial relaxing and contracting factors (Figure 3b). Neuroeffector junctions do not have a permanent geometry with postjunctional specializations, but rather the varicosities are continuously moving and their special relation with muscle cell membranes changes with time, including dispersal and reformation of receptor clusters (14). For example, varicosity movement is likely to occur in cerebral blood arteries, where there is a continuously increasing density of sympathetic innervation during development and aging, and in hypertensive vessels or those that have been stimulated chronically in vivo, where there is an increase in innervation density of up to threefold.…”

Section: Definition Of the Autonomic Neuroeffector Junctionmentioning

confidence: 99%

Autonomic Neurotransmission: 60 Years Since Sir Henry Dale

Burnstock

2009

Annu. Rev. Pharmacol. Toxicol.

110

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In the early twentieth century, Sir Henry Dale and others described brilliant studies of autonomic neurotransmission utilizing acetylcholine and noradrenaline. However, within the past 60 years, new discoveries have changed our understanding of the organization of the autonomic nervous system, including the structure and function of the nonsynaptic autonomic neuroeffector junction, the multiplicity of neurotransmitters, cotransmission, neuromodulation, dual control of vascular tone by perivascular nerves and endothelial cells, the molecular biology of receptors, and trophic signaling. Further, it is now recognized that an outstanding feature of autonomic neurotransmission is the inherent plasticity afforded by its structural and neurochemical organization and the interaction between expression of neural mediators and environmental factors. In this way, autonomic neurotransmission is matched to ongoing changes in demands and can sometimes be compensatory in pathophysiological situations.

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P2X (Purinergic) receptor distributions in rat blood vessels

Cited by 96 publications

References 46 publications

Physiological significance of P2X receptor-mediated vasoconstriction in five different types of arteries in rats

Physiological significance of P2X receptor-mediated vasoconstriction in five different types of arteries in rats

Immunohistochemical identification of cells expressing ATP‐gated cation channels (P2X receptors) in the adult rat thyroid

Autonomic Neurotransmission: 60 Years Since Sir Henry Dale

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