P2Y Receptors Regulate Proliferation of Human Pancreatic Duct Epithelial Cells

Abstract

Extracellular nucleotides increase proliferation of human pancreatic duct epithelial cells by activation of P2Y(1) and P2Y(6) receptors. This provides the basic model for the effect of P2Y receptors on the proliferation of pancreatic duct epithelial cells.

Uridine Triphosphate Increases Proliferation of Human Cancerous Pancreatic Duct Epithelial Cells by Activating P2Y2 Receptor

“…Pancreatic duct epithelial cells were pretreated with different inhibitors of signaling pathway that may mediate the increase of proliferation by P2Y 2 receptor. 1,11,13,14,16,19 As shown in Figure 4 Recent studies showed that Akt serine/threonine kinase is a key component of proliferative pathway in pancreatic cancer cells, breast cancer cells, and osteoblastic cells. 33Y35 To determine whether Akt is modulated by P2Y 2 receptor, UTP (10 Kmol/L) was applied to PANC-1 cells for 5 minutes.…”

“…1 In addition, UTP, an agonist of the P2Y 2 and P2Y 4 receptors, exerted less proliferative activity than adenosine diphosphate and uridine diphosphate, and the expression level of P2Y 2 receptor mRNA was higher than those of other P2Y receptors. In this study, the proliferative role of the P2Y 2 receptor and proliferative signaling pathway in pancreatic duct epithelial cells were investigated.…”

“…The presence of the P2Y 2 receptor in pancreatic cells is in agreement with the findings of previous studies. 1,3,9 It was further attempted to identify the involvement of the P2Y 2 receptor by application of the P2 receptor antagonists, suramin and PPADS (Fig. 3).…”

“…1 Briefly, PANC-1 cells were seeded at a density of 1 Â 10 4 cells per well on 96-well plates and incubated for 24 hours. The cells were then incubated with different nucleotides for 24 hours.…”

“…3 In addition, these P2 receptors can affect long-term cellular processes of the pancreas, such as cell growth. 1 The P2Y 2 receptor that regulates pancreatic secretion plays key short-term roles in various tissues and cells. It regulates secretion, contraction, and intracellular signaling such as intracellular Ca 2+ , kinase, and phospholipase.…”