P2Y1R and P2Y2R: potential molecular triggers in muscle regeneration

Wang, Min-Jia; Yang, Bi-Ru; Jing, Xin-Yu; Wang, Yao-Zheng; Lu, Kang; Ren, Kai; Kang, Liang

doi:10.1007/s11302-022-09885-z

Cited by 7 publications

(5 citation statements)

References 74 publications

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“…In general, the high-water permeability of cell membranes causes rapid osmotic responses via the movement of osmotically active ions into or out of cells. Hypertrophy of the cell volume is observed when signaling from P2Y2 is transduced in muscles [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling

et al. 2023

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In bacteria, polymers of inorganic phosphates, particularly linear polyphosphate, are used as alternative phosphate donors for adenosine triphosphate production. A six-chain form of sodium metaphosphate, sodium hexametaphosphate (SHMP), is believed to have no physiological functions in mammalian cells. In this study, we explored the possible effects of SHMP on mammalian cells, using mouse oocytes, which are useful for observing various spatiotemporal intracellular changes. Fertilization-competent oocytes were isolated from the oviducts of superovulated mice and cultured in an SHMP-containing medium. In the absence of co-incubation with sperm, SHMP-treated oocytes frequently formed pronuclei and developed into two-cell embryos owing to the increase in calcium concentration in the cytoplasm. We discovered an intriguing role for SHMP as an initiator of calcium rise in mouse oocytes, presumably in a wide variety of mammalian cells.

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Section: Discussionmentioning

confidence: 99%

Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling

et al. 2023

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“…We show that cellular stress (as induced by pro‐inflammatory LPS) causes an increase in CTKs associated with the tRNA degradation pathway. The increased presence of CTKs in response to LPS is particularly relevant given the importance of purinergic signaling in skeletal muscle (Fulle, 2015 ; Wang et al., 2023 ) and the putative role for tRNA‐derived fragments in regulating skeletal muscle homeostasis and stress responses (Liapi et al., 2020 ). Collectively, iP forms were the most abundant of all CTKs detected both within and outside the cell (Figure 4 ) and all forms of iP (FB, RB, and NT) were elevated in response to LPS treatment in the extracellular space (chemical structures of iP as a FB, RB, or NT are summarized in Figure 5a ).…”

Section: Discussionmentioning

confidence: 99%

Profiling of adenine‐derived signaling molecules, cytokinins, in myotubes reveals fluctuations in response to lipopolysaccharide‐induced cell stress

Tobin,

Seneviratne,

Phan

et al. 2023

Physiological Reports

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Cytokinins (CTKs) are a diverse collection of evolutionarily conserved adenine‐derived signaling molecules classically studied as phytohormones; however, their roles and production have been less studied in mammalian systems. Skeletal muscles are sensitive to cellular cues such as inflammation and in response, alter their secretome to regulate the muscle stem cell and myofiber niche. Using cultured C2C12 muscle cells, we profiled CTK levels to understand (1) whether CTKs are part of the muscle secretome and (2) whether CTKs are responsive to cellular stress. To induce cellular stress, C2C12 myotubes were treated with lipopolysaccharides (LPS) for 24 h and then media and cell fractions were collected for ultra high‐performance liquid chromatography tandem mass spectrometry with electrospray ionization (UHPLC‐(ESI+)‐HRMS/MS) for metabolomics and CTK profiling. Across LPS‐treated and control cells, 11 CTKs were detected in the extracellular space while 6 were detected intracellularly. We found that muscle cells are enriched in isopentenyladenine (iP) species (from free base, riboside to nucleotide forms), and that extracellular levels are increased after LPS treatment. Our study establishes that muscle cells express various forms of CTKs, and that CTK levels are responsive to LPS‐induced cell stress, suggesting a role for CTKs in intra‐ and extracellular signaling of mammalian cells.

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“…The action of P2Y receptors is controversial. P2Y1 and P2Y2 receptors improve muscle regeneration, which is blocked when these receptors are inhibited [112]. However, in skeletal muscle fibroblasts, P2Y2 receptors promote fibrosis and muscle atrophy via Akt/ERK/PKC activation [113].…”

Section: P2 Receptors In Muscle and Fat In Sarcopeniamentioning

confidence: 99%

Energy Regulation in Inflammatory Sarcopenia by the Purinergic System

Marco-Bonilla,

Fresnadillo,

Largo

et al. 2023

IJMS

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The purinergic system has a dual role: the maintenance of energy balance and signaling within cells. Adenosine and adenosine triphosphate (ATP) are essential for maintaining these functions. Sarcopenia is characterized by alterations in the control of energy and signaling in favor of catabolic pathways. This review details the association between the purinergic system and muscle and adipose tissue homeostasis, discussing recent findings in the involvement of purinergic receptors in muscle wasting and advances in the use of the purinergic system as a novel therapeutic target in the management of sarcopenia.

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P2Y1R and P2Y2R: potential molecular triggers in muscle regeneration

Cited by 7 publications

References 74 publications

Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling

Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling

Profiling of adenine‐derived signaling molecules, cytokinins, in myotubes reveals fluctuations in response to lipopolysaccharide‐induced cell stress

Energy Regulation in Inflammatory Sarcopenia by the Purinergic System

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