P37.15 Prevalence of Targetable Mutations using a RT-qPCR Multiplex Assay in Asian NSCLC Patients: A Single Laboratory Experience

Abstract

We derived a 7-marker and 5-marker classifier using the Luminex assay and multiplex realtime PCR, respectively. The 5-protein panel assay was comprised of epidermal growth factor receptor 1, matrix metalloproteinase 7, KIT, and complement component 9. The 5-marker classifier resulted in 66.7% sensitivity with 92.2% specificity for whole stage, and 44.4% sensitivity with 92.2% specificity for stage I and II disease. Conclusion: We have developed a 5-protein biomarker panel using multiplex real-time PCR to accurately identify NSCLC. The panel displayed similar diagnostic performance compared to a 7-protein biomarker-based Luminex assay. The high specificity in patients with NSCLC might help differentiate non-malignant pulmonary nodules in low-dose chest computed tomography screening.