p38 MAPK activation is required for esculetin-induced inhibition of vascular smooth muscle cells proliferation

Yun, Eun-Sun; Park, Sung-Suk; Shin, Ho‐Chul; Choi, Yung Hyun; Kim, Wun-Jae; Moon, Sung‐Kwon

doi:10.1016/j.tiv.2011.05.001

Cited by 34 publications

(26 citation statements)

References 34 publications

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“…IGF1R is a protein and transmembrane tyrosine protein receptor which is involved in the IGF1/MAPK signalling pathway [48,49]. P38MAPK, an important member of the MAPK signalling pathway, can activate the MAPK signalling pathway [50][51][52][53], causing the proliferation of cells [54][55][56]. Moreover, our previous studies have shown that p38MAPK signalling is inhibited by miR-451 in early DN [15].…”

Section: Discussionmentioning

confidence: 99%

The Long Noncoding RNA 150Rik Promotes Mesangial Cell Proliferation via miR-451/IGF1R/p38 MAPK Signaling in Diabetic Nephropathy

Zhang¹,

Sun²,

Peng³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Diabetic nephropathy (DN) as the primary cause of end-stage kidney disease is a common complication of diabetes. However, the initiating molecular events triggering DN are unknown. Recently, long noncoding RNAs (lncRNAs) have been shown to play important roles in DN. Methods: The expression level of lncRNA 1500026H17Rik (150Rik for short) was measured by qRT-PCR (quantitative real-time PCR). Cell proliferation ability was detected by 5-Ethynyl-2’-deoxyuridine (EdU). The relationship between 150Rik and microRNA 451 (miR-451) was examined by luciferase assay and RNA immunoprecipitation (RIP) assay. Finally, the effect of 150Rik on cell proliferation through the miR-451/insulin-like growth factor 1 receptor (IGF1R)/mitogen-activated protein kinases (p38MAPK) pathway was detected by EdU, flow cytometry analysis, western blot. Results: We found that 150Rik, an evolutionarily conserved lncRNA, was significantly upregulated in renal tissue of db/db DN mice and in mesangial cells (MCs) cultured under a high glucose condition. Further, overexpression or knockdown of 150Rik was found to regulate cell proliferation in MCs. Moreover, 150Rik was found to interact with miR-451 in both a direct and argonaute-2 (Ago2)-dependent manner. Results also revealed that overexpression of 150Rik inhibited cell proliferation through the miR-451/IGF1R/p38MAPK pathway in MCs under the high glucose condition, while knockdown of 150Rik increased cell proliferation via the miR-451/IGF1R/p38MAPK pathway. Conclusion: Taken together, these results provide new insight into the association between 150Rik and the miR-451/IGF1R/p38MAPK signaling pathway during DN progression.

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Section: Discussionmentioning

confidence: 99%

The Long Noncoding RNA 150Rik Promotes Mesangial Cell Proliferation via miR-451/IGF1R/p38 MAPK Signaling in Diabetic Nephropathy

Zhang¹,

Sun²,

Peng³

et al. 2018

Cell Physiol Biochem

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“…Environmental conditions and seasonal changes may affect the incidence of coumarins in various parts of the plant (4). Among coumarins, a number of compounds have been reported to exhibit anticancer properties, including imperatorin (5), esculetin (6) and osthole, which inhibits the migration and invasion of breast cancer cells, potentially via the inhibition of matrix metalloproteinase promoter and enzymatic activity (7). Another type of 4-phenylcoumarins isolated from C. inophyllum is calocoumarin A (8).…”

Section: Calophyllum Inophyllum L (C Inophyllum) Of the Familymentioning

confidence: 99%

“…The absorbance of this colored solution can be quantified using a specific wavelength (550-600 nm) and a spectrophotometer. The cells (1x10 6 ) were cultured in 60 mm tissue culture dishes at 37˚C for 24 h. The culture medium was replaced with new medium, and the cells were exposed to various concentrations, as above, of the green or yellow pigments at 37˚C for 24, 48 and 72 h. Following treatment, the cells were incubated with 0.5 mg/ml MTT for 2 h at room temperature and then lysed with DMSO at room temperature for 5 min. A 200 µl aliquot of the resulting solution was then removed from the 60 mm culture dishes and transferred to 96-well plates.…”

Section: Analysis Of Seed Oil Pigments From C Inophyllum Lmentioning

confidence: 99%

“…The cells (1x10 6 ) were cultured in 60 mm tissue culture dishes at 37˚C for 24 h. The RPMI 1640 culture medium (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) was replaced with fresh medium, and the cells were exposed to 0.60, 0.75 and 1.50x10 -3 % of the yellow pigment and 5.0, 12.5 and 25.0x10 -3 % of the green pigment at 37˚C for 48 h. Subsequently, the cells were pooled, washed with phosphate-buffered saline (PBS), fixed in a PBS-methanol (volume/volume, 1:2) solution at room temperature for 5 min and then incubated at 4˚C for at least 18 h. Following washing with PBS once, the cell pellets were stained with the PI solution supplemented with PBS, 40 µg/ml PI and 40 µg/ml of DNase-free RNase A (Sigma-Aldrich; Merck KGaA) for 30 min at room temperature in the dark and then analyzed using a Becton-Dickinson FACScan flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). A total of ≥10,000 cells were counted per sample, and the DNA histograms were further evaluated using the Modfit software version no.…”

Section: Analysis Of Seed Oil Pigments From C Inophyllum Lmentioning

confidence: 99%

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Yellow and green pigments from Calophyllumï¿½inophyllum L. seed oil induce cell death in colon and lung cancer cells

et al. 2018

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Abstract. Natural compounds have been candidates for anticancer medicine over the last 20 years. During the process of isolating seed oil from Calophyllum inophyllum L., yellow and green pigments containing multiple compounds with an aromatic structure were identified. High-performance liquid chromatography and nuclear magnetic resonance analysis of these pigments revealed that the compounds present were identical, but the concentration of the compounds was different. Treatment with the pigments was able to induce the death of DLD-1 human colon cancer cells and increase the percentage of the cells in the sub-G 1 and sub-G 2 /M phases in a dose-dependent manner. Additionally, the pigments were able to exhibit cytotoxic activity on A549 and H1975 human non-small cell lung cancer (NSCLC) cell lines at 24 h, with half-maximal inhibitory concentrations (IC 50 ) values of 0.1206 and 0.0676%, respectively for green pigments, and 0.0434 and 0.0501%, respectively for yellow pigments. Furthermore, a decrease in IC 50 value was associated with an increase in the duration of treatment. However, a sharp decrease in IC 50 value of the yellow pigment was observed for H1975 cells at 48 h and for A549 cells at 72 h compared with no change in IC 50 value for the green pigment with time, suggesting that the pigments function and induce cell death differently in the two cell lines. An investigation was performed into the synergistic effect of the green pigment and gefitinib (Iressa ® , ZD1839), which is a selective epidermal growth factor receptor-tyrosine kinase inhibitor to block growth factor-mediated cell proliferation. The combination of the green pigment and gefitinib resulted in an enhancement of the decrease in viability of A549 and H1975 cells compared with treatment with gefitinib alone, which suggested that treatment with the green pigments was able to enhance the sensitivity of NSCLC cells to gefitinib. In conclusion, these pigments may be considered for development as anti-colon cancer agents.

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“…Esculetin (6, 7-dihydroxycoumarin) is present in many plants which are commonly used as a folk medicine to counteract various inflammatory, allergic and infectious diseases. It has an inhibitory effect on lipoxygenase and cyclooxygenase enzymatic cascades (Tomohiro, Yasuko, & Sei-Itsu, 1983), ability to scavenge reactive oxygen species (Prabakaran & Ashokkumar, 2013), antifibrotic (Surse, Gupta, & Tikoo, 2011), and anti-proliferative potential (Lee et al, 2013;Yun et al, 2011). Esculetin was found to be protective against hepatic and renal dysfunction associated with diabetes in STZ induced type I diabetic rats (Prabakaran & Ashokkumar, 2012Surse et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Esculetin reverses histone H2A/H2B ubiquitination, H3 dimethylation, acetylation and phosphorylation in preventing type 2 diabetic cardiomyopathy

Kadakol

Malek

Goru

et al. 2015

Journal of Functional Foods

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p38 MAPK activation is required for esculetin-induced inhibition of vascular smooth muscle cells proliferation

Cited by 34 publications

References 34 publications

The Long Noncoding RNA 150Rik Promotes Mesangial Cell Proliferation via miR-451/IGF1R/p38 MAPK Signaling in Diabetic Nephropathy

The Long Noncoding RNA 150Rik Promotes Mesangial Cell Proliferation via miR-451/IGF1R/p38 MAPK Signaling in Diabetic Nephropathy

Yellow and green pigments from Calophyllumï¿½inophyllum L. seed oil induce cell death in colon and lung cancer cells

Esculetin reverses histone H2A/H2B ubiquitination, H3 dimethylation, acetylation and phosphorylation in preventing type 2 diabetic cardiomyopathy

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