p53 codon 72 polymorphism in basal cell carcinoma of the skin

Pezeshki, Abdulmohammad; Aslani, Fatemeh Sari; Ghaderi, Abbas; Doroudchi, Mehrnoosh

doi:10.1007/bf02893428

Cited by 21 publications

(21 citation statements)

References 25 publications

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“…A previous study which analyzed the correlation between the p.R72P polymorphism and p53 mutation in breast cancer patients, has reported that p53 mutation was more prevalent in the Arg/Arg genotype than those of the Pro/ Pro genotype (Langerod et al, 2002). The Arg allele at codon 72 of the TP53 has been suggested to affect the risk of UV-induced basal cell carcinoma (Pezeshki et al, 2006) as the frequency of the Arg allele was significantly higher in sun-exposed patients compared to controls.…”

Section: Discussionmentioning

confidence: 99%

p.R72P, PIN3 Ins16bp Polymorphisms of TP53 and CCR5Δ32 in North Indian Breast Cancer Patients

Guleria¹,

Sharma²,

Manjari³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 99%

p.R72P, PIN3 Ins16bp Polymorphisms of TP53 and CCR5Δ32 in North Indian Breast Cancer Patients

Guleria¹,

Sharma²,

Manjari³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…Pezeshki et al (21) demonstrated that the frequency of the Arg allele was significantly higher in sun-exposed patients compared to controls, suggesting that Arg allele at codon 72 of the p53 gene might affect the risk of UV-induced basal cell carcinoma.…”

Section: Discussionmentioning

confidence: 99%

CCR5 and p53 codon 72 gene polymorphisms: Implications in breast cancer development

Watanabe¹

2009

Int J Mol Med

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Abstract. The aim of this study was to investigate the CCR5 gene and p53 codon 72 polymorphisms in a Brazilian population with breast cancer compared with healthy control subjects and to associate the clinical stage with these genotypes. No differences were detected for the D32 allele between breast cancer patients and the normal healthy donors (p=0.270), although this allele was more often present in blood donors. For p53 genotype analysis, breast cancer patients presented a significant (p<0.05) over-representation of p53 Arg homozygosity (55.5%) compared with the healthy control group (33.3%). Although no statistical difference occurred, a very strong tendency in breast cancer patients in stage III (p=0.0503) presenting homozygous genotype for Arg was verified. Five breast cancer patients were D32 deletion carriers and two patients presenting metastasis showed homozygous genotype for Arg. It is possible that p53 Arg homozygosity is associated with breast cancer and may represent a potential risk factor for breast tumorigenesis. In the present study, a higher percentage of breast cancer patients presented homozygous genotype for Arg and wild-type for CCR5 than the control subjects.

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“…Genotyping of p53 codon 72. p53 codon 72 polymorphism detection was carried out by an allele specific PCR described by Pezeshki et al (31) with slight modification on a Palm Cycler (Corbett Research, Australia). To detect the p53 codon 72 polymorphism, two primer sets in separate tubes were used, one to amplify the Arg allele and the other to amplify the Pro allele as follows: Arg forward, TCC CCC TTg CCg TCC CAA, Arg reverse, CTg gTg Cag ggg CCA CgC; Pro forward, gCC AgA ggC TgC TCC CCC, Pro reverse, CgT gCA AgT CAC AgA CTT.…”

Section: Studymentioning

confidence: 99%

“…conducted to find these association since most of the studies tried to analyze the associations of the polymorphism in cancer patients and healthy population (31,34). Moreover, Arg/ Arg genotype was found dominant in both types of NMSC.…”

Section: B Amentioning

confidence: 99%

p53 codon 72 polymorphisms and random amplified polymorphic DNA analysis of non-melanoma skin cancer through archival formalin-fixed paraffin-embedded tissue

et al. 2011

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Abstract. Non-melanoma skin cancer (NMSC) is classified among the ten most frequent cancers in Malaysia. A common polymorphism at codon 72 of the p53 tumor suppressor gene and its influence on cancer risk has been studied for different types of cancer with mixed and inconsistent results with limited published data on the Malaysian population so far. In the present study, the frequency of p53 codon 72 polymorphism in 60 patients with NMSC was investigated from archival formalin-fixed paraffin-embedded (FFPE) tissue obtained from Hospital Universiti Kebangsaan Malaysia (HUKM). Additionally, random amplified polymorhic DNA -polymorphic chain reaction (RAPD-PCR) was employed for preliminary biomarker development. NMSC FFPE samples (70%) possess Arg/Arg, 20% with Pro/Pro and 10% with Arg/ Pro. In total, there was no significant difference in the p53 codon 72 genotypes between histological types of NMSC, gender, race, tumor location and age group. However, there was an apparent age-associated increase in the Arg/Arg genotype but did not reach statistical significance (P= 0.235). NMSC types and demographic characteristics did not influence genotype distribution. On the other hand, BCC and SCC distributions are influenced by age group, race and tumor location. IntroductionNon-melanoma skin cancer (NMSC) is the most common type of cancer among different populations worldwide.Consisting of two histological types i.e. basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), the BCC accounts for 80% and SCC for the rest (1). Regardless of its low clinical aggressiveness, due to its frequency and cost of treatment, it is a heavy public health burden (2). In Malaysia, official statistics rank NMSC among the ten most common types of cancer in adults (3). Exposure to sun is considered the main causative agent for NMSC (4). However, it cannot account for all of NMSC etiology since other physical and chemical agents, such as ionizing radiation, arsenic and coal tar products, as well as suppression of immune system in transplant patients, are verified causes of NMSC (5). Furthermore, genetic polymorphism is proposed to modify the risk of the disease (6).The p53 tumor suppressor protein plays an essential part in maintaining cellular integrity and tissue homeostasis through its ability to orchestrate the transcriptional activation of other genes (7). A critical region of p53 for signaling apoptosis lies between codons 64 and 92, encoding a proline-rich region of the gene homologous to an SH3 binding domain (8-10) in which there is a common polymorphism resulting in either an arginine (CGC) or a proline (CCC) at codon 72 of exon 4. This is a non-conservative amino acid change and results in a structural change in the protein as the p53Pro variant migrates more slowly than the p53Arg variant in sodium dodecyl sulfatepolyacrylamide gel electrophoresis (11). The two alleles of p53 differ biologically. p53Arg induces 5-10 times more apoptosis than p53Pro, which is explained in part by a higher ability of p53Arg to localize to the m...

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p53 codon 72 polymorphism in basal cell carcinoma of the skin

Cited by 21 publications

References 25 publications

p.R72P, PIN3 Ins16bp Polymorphisms of TP53 and CCR5Δ32 in North Indian Breast Cancer Patients

p.R72P, PIN3 Ins16bp Polymorphisms of TP53 and CCR5Δ32 in North Indian Breast Cancer Patients

CCR5 and p53 codon 72 gene polymorphisms: Implications in breast cancer development

p53 codon 72 polymorphisms and random amplified polymorphic DNA analysis of non-melanoma skin cancer through archival formalin-fixed paraffin-embedded tissue

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