p53 expression and apoptosis in liver and spleen during CO2 pneumoperitoneum

Arıkan, Yüksel; Tosun, Murat; Şaykol, Volkan; Kalkan, Serpil; Erdem, Serpil

doi:10.1007/s00423-007-0254-6

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…It is known that apoptosis is a very important contributor in kidney damage during renal I/R injury (25). p53 is a guardian of the genome and DNA damage induces its expression (1). In this study, it was found that there was increase in p53 expression in I/R group, while it was decreased in Dc group showing protective effect of Dc on DNA damage.…”

Section: Discussionsupporting

confidence: 43%

Protective effects of doxycycline in ischemia/reperfusion injury on kidney

et al. 2009

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Renal ischemia and reperfusion injury is the major cause of acute renal failure and may also be involved in the development and progression of some forms of chronic kidney disease. The aim of this study was to evaluate whether doxycycline, a member of the tetracycline family of antibiotics, protects kidney tissue or not. 36 Sprague-Dawley rats (200-250 g) were used. The animals were divided into three groups: control, ischemia/reperfusion and ischemia/reperfusion+doxycycline group. Rats were subjected to renal ischemia by clamping the left pedicle for 1 h, and then reperfused for 1 h. The ischemia/reperfusion+doxycycline group were pretreated intraperitoneally with doxycycline suspension (10 mg/kg) 2 h before the induction of ischemia. Our results indicate that malondialdehyde, matrix-metalloproteinase-2, interleukin-2, interleukin-6, interleukin-10, interleukin 1-beta and tumor necrosis factor-alpha levels were significantly higher in the ischemia/reperfusion group than those in the control group. Doxycycline administration significantly decreased these parameters. Tissue inhibitor of metalloproteinases-1 levels also increased after ischemia/reperfusion and decreased with doxycycline pretreatment, but these changes were not significantly different. Glutathione levels significantly decreased after ischemia/reperfusion injury when compared with the control group and doxycycline pretreatment significantly increased glutathione levels when compared with the ischemia/reperfusion group. Apoptotic cells and p53 positive cells were significantly decreased in doxycycline treated group. These results suggest that doxycycline reduces renal oxidative injury and facilitates repair. Doxycycline may play a role in a renoprotective therapeutic regimen.

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Section: Discussionsupporting

confidence: 43%

Protective effects of doxycycline in ischemia/reperfusion injury on kidney

et al. 2009

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“…It means that CO 2 pneumoperitoneum in higher pressures have lethal effects on kidney tubular cells. Generally, apoptotic pathways were activated by p53 expression in our before studies [10][11][12][13][14].However, in this study, we detected that p53 expression was detected only 20 mmHg applied groups. This protection might be related with mechanical resistance of renal capsule and also retroperitoneal location of kidneys.…”

Section: Discussioncontrasting

confidence: 82%

“…However, it was revealed that the side effects of CO 2 pneumoperitoneum such as organ ischemia, cell death, physiopathological changes were minimal and reversible without any apparent sequel when the shorter time and lower pressure was used. Because we have studied the effects of CO 2 pneumoperitoneum in different intraabdominal organs in our different studies before [10][11][12][13][14], we have standardized CO 2 pressure for experimental studies.…”

Section: Discussionmentioning

confidence: 99%

P53 related apoptosis in kidneys in CO2 pneumoperitoneum rat model: an immunohistochemical study

et al. 2014

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Laparoscopic surgery techniques have been increasingly preferred to classic laparotomy by surgeons since 1987. However, this method may have some side effects on different intraabdominal organs including kidneys. The aim of this study is to evaluate the effects of different pressures of CO2 on p53 related apoptosis in kidneys. Totally 24 male rats were divided into four equal groups. CO2 is insufflated into rats' intraabdominal cavity in two different pressures of 10 and 20 mmHg during 1 h. However, in sham group, only cannula was inserted, but no gas was insufflated. After 1 h, 30 min reperfusion was applied. At last, the kidneys were excised and p53 expression and apoptosis were evaluated immunohistochemically. All the data revealed that the number of apoptotic cell in kidney' tubular cells significantly increases in proportion to CO2 pressure level. On the other hand, p53 expression was detected only in the highest pressure. Because the low CO2 pressured group' rats had no p53 expression in kidneys, we suggest that this method can be safely used for abdominal surgery. At the same time, increasing in the number of apoptotic cells parallel to pressure also suggest that CO2 pressure level and application time are very important parameters during CO2 pneumoperitoneum.

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“…[14][15][16] On the other hand, it has been postulated that the use of low pneumoperitoneum pressure (LPP) during laparoscopy could be peritoneum friendly and reduce some of these undesired effects. [17][18][19][20][21] Nevertheless, the impact of lowering intra-abdominal pressure on surgical space conditions and safety has not yet been well established, hence the routine use of this approach still remains controversial. 22 We hypothesized that the use of LPP (8 mm Hg) during laparoscopic cholecystectomy could minimize the adverse impact on the surgical peritoneal environment [measured as gene expression of extracellular matrix adhesion and inflammatory cytokines and apoptotic index (AI)] and improve clinical outcomes.…”

mentioning

confidence: 99%

“…On the other hand, it has been postulated that the use of low pneumoperitoneum pressure (LPP) during laparoscopy could be peritoneum friendly and reduce some of these undesired effects 17–21 . Nevertheless, the impact of lowering intra-abdominal pressure on surgical space conditions and safety has not yet been well established, hence the routine use of this approach still remains controversial 22 .…”

mentioning

confidence: 99%

Impact of Standard Versus Low Pneumoperitoneum Pressure on Peritoneal Environment in Laparoscopic Cholecystectomy. Randomized Clinical Trial

Serrano,

Díaz-Cambronero,

Montiel

et al. 2023

Surgical Laparoscopy, Endoscopy &Amp; Percutaneous Techniques

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Background: High CO2 pneumoperitoneum pressure during laparoscopy adversely affects the peritoneal environment. This study hypothesized that low pneumoperitoneum pressure may be linked to less peritoneal damage and possibly to better clinical outcomes. Materials and Methods: One hundred patients undergoing scheduled laparoscopic cholecystectomy were randomized 1:1 to low or to standard pneumoperitoneum pressure. Peritoneal biopsies were performed at baseline time and 1 hour after peritoneum insufflation in all patients. The primary outcome was peritoneal remodeling biomarkers and apoptotic index. Secondary outcomes included biomarker differences at the studied times and some clinical variables such as length of hospital stay, and quality and safety issues related to the procedure. Results: Peritoneal IL6 after 1 hour of surgery was significantly higher in the standard than in the low-pressure group (4.26±1.34 vs. 3.24±1.21; P=0.001). On the contrary, levels of connective tissue growth factor and plasminogen activator inhibitor-I were higher in the low-pressure group (0.89±0.61 vs. 0.61±0.84; P=0.025, and 0.74±0.89 vs. 0.24±1.15; P=0.028, respectively). Regarding apoptotic index, similar levels were found in both groups and were 44.0±10.9 and 42.5±17.8 in low and standard pressure groups, respectively. None of the secondary outcomes showed differences between the 2 groups. Conclusions: Peritoneal inflammation after laparoscopic cholecystectomy is higher when surgery is performed under standard pressure. Adhesion formation seems to be less in this group. The majority of patients undergoing surgery under low pressure were operated under optimal workspace conditions, regardless of the surgeon’s expertise.

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p53 expression and apoptosis in liver and spleen during CO2 pneumoperitoneum

Abstract: CO(2) pressure level and application time may affect on cells living in liver and spleen. High pressure and/or long application time may cause releasing of cytokines and superoxide radicals from these organs' cells, and transient or serious organ dysfunctions may occur.

Cited by 6 publications

References 14 publications

Protective effects of doxycycline in ischemia/reperfusion injury on kidney

Protective effects of doxycycline in ischemia/reperfusion injury on kidney

P53 related apoptosis in kidneys in CO2 pneumoperitoneum rat model: an immunohistochemical study

Impact of Standard Versus Low Pneumoperitoneum Pressure on Peritoneal Environment in Laparoscopic Cholecystectomy. Randomized Clinical Trial

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