p53-induced Gene 3 Mediates Cell Death Induced by Glutathione Peroxidase 3

Abstract

Background: Glutathione peroxidase 3 was identified as a new tumor suppressor. However, the mechanism is not clear. Results: Our analysis indicates that glutathione peroxidase 3 activates p53-induced gene 3 both in vivo and in vitro. Conclusion: p53-induced gene 3 is a major mediator of GPx3-induced cell death. Significance: Glutathione peroxidase 3-p53-induced gene 3 signaling represents a novel signaling pathway for cell death.

Global methylation analysis identifies prognostically important epigenetically inactivated tumor suppressor genes in multiple myeloma

“…36 Underlining its tumor-suppressive role, GPX3 is frequently deleted or silenced by hypermethylation in solid tumors. 37,38 Our data also indicate a prognostic role of impaired retinoic acid (RA) signaling in myeloma.…”

The DNA Replication Licensing Factor Miniature Chromosome Maintenance 7 Is Essential for RNA Splicing of Epidermal Growth Factor Receptor, c-Met, and Platelet-derived Growth Factor Receptor

“…Yeast Two-hybrid Analysis-The yeast-competent cell preparation was previously described (15,21,(27)(28)(29)(30). One hundred microliters of freshly prepared competent AH109 cells were mixed with plasmid DNA (0.25-0.50 g) plus 0.5 g of DNA from prostate yeast two-hybrid cDNA library constructed in pACT2 in 0.5 ml of polyethylene glycol/LiAc, incubated at 30°C for 30 min.…”

Oxidants, antioxidants and the current incurability of metastatic cancers

“…Although looking for genes expressed only during apoptosis, they discovered a set of 13 p53-induced genes ( PIG genes), each of which are likely key players in the cellular synthesis of reactive oxygen species (ROS; H 2 O 2 hydrogen peroxide, the OH − radiation and O 2 − superoxides). PIG3 , for example, codes for a quinone oxidoreductase that is a potent generator of ROS [30,31]. p53 target genes also play major roles in downstream processes through turning on synthesis of some 10 different mitochondrial functioning proteins such as BAX, PUMA and NOXA, as well as death receptors such as DR4 and DR5, that in ways yet to be elucidated help carry out the many successive proteolysis stages in apoptosis [32].…”