p53 Protein Expression in Odontogenic Cysts

Abstract

p53 protein seems to be related to the suppression of cell proliferation. p53-positive tissues seem to have a higher proliferative activity than p53-negative ones. Odontogenic keratocyst (OKC) has a different behavior from other types of cysts because it is more aggressive, with a tendency to recurrence. Twenty-two dentigerous cysts, 24 radicular cysts, and 20 OKCs were used in the present study. Two dentigerous cysts (9.1%), 2 radicular cysts (8.3%), and 9 OKCs (45%) expressed the p53 protein. The differences between the three groups were statistically significant (p = 0.003). In 10 cases of OKCs epithelial dysplasia was found. One of the 10 OKCs without dysplasia and 8 of the 10 OKCs with dysplasia were p53-positive: the difference between the two groups was statistically significant (p = 0.007). The overexpression of p53 protein was not on the other hand correlated with the occurrence of multiple, bilateral, and recurrent OKCs. Moreover the distribution of p53-positive cells was parabasal in contrast with other types of cysts. These qualitative and quantitative differences in proliferative activity in OKCs seem to point to an alteration in cell cycle control.

Immunohistochemical comparative study of the odontogenic keratocysts and other odontogenic lesions

“…In this study, p53 immunoexpression was positive in 50% of OKCs, 40% of dentigerous cysts and radicular cysts, and 30% of ameloblastomas, but without statistically significant differences among them. Conversely, Piatelli et al (19), also found a higher p53 expression in odontogenic keratocysts than in dentigerous and radicular cysts, and differences between the three groups were stastistically significant. Ogden et al (17) using CM-1 antibody reported that 5 of 12 odontogenic keratocysts expressed P53, while Lombardi et al (20) with the same clone CM-1 found p-53 in 50% of their OKCs, including non-recurrent, recurrent and associated with the basalcell nevus syndrome.…”

“…Ogden et al (17) using CM-1 antibody reported that 5 of 12 odontogenic keratocysts expressed P53, while Lombardi et al (20) with the same clone CM-1 found p-53 in 50% of their OKCs, including non-recurrent, recurrent and associated with the basalcell nevus syndrome. Piatelli et al (19), employing the clone DO7 found p-53 expression in 45% of 20 odontogenic keratocysts. We have found the same percentage of positively stainded cells in OKCs as these authors on the contrary we have found higher positivity rates in dentigerous cysts and radicular cysts.…”

Etude immunohistochimique des facteurs de la prolifération (Ki67, PCNA) et de l’apoptose (P53) sur 40 kératokystes odontogénes

“…En conséquence, certains auteurs se sont orientés vers l'étude de l'expression de la P53 en cherchant à établir l'existence d'une corrélation entre l'expression de cette protéine et la survenue récidive des KKO [4][5][6]. Dans cette étude, 10 cas étaient positifs pour la P53 ; comme cela a déjà été précisé [4,[6][7][8][9][10][11], cette positivité a été observée dans les couches suprabasales de l'épithélium.…”

“…La comparaison de l'expression de la P53 dans les diffé-rentes formes cliniques n'a pas montré de différence statistiquement significative (chi-2 = 0.191). Nos résultats concordent avec ce qui a déjà été publié [3,8,9,11]. Dans les KKO, il existe une l'hyperexpression de la P53 par rapport aux autres kystes odontogénes.…”

Odontogenic keratocysts/keratocystic odontogenic tumours: biological characteristics, clinical manifestation and treatment

“…Relatively frequent was focus on the significance of suppressor gene p53 expression 6 . The results showed that immunohistochemically detected overexpression of p53 was caused more by overproduction or increased stabilization of the protein than gene amplification.…”