P53‐regulated lncRNA uc061hsf.1 inhibits cell proliferation and metastasis in human esophageal squamous cell cancer

Yao, Juan; Zhang, Hao; Li, Hua; Qian, Rong-Yu; Liu, Ping; Huang, Junxing

doi:10.1002/iub.2196

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…p53 is frequently inactivated or lowly expressed in human cancer, and it is able to directly bind to gene promoters to promote or inhibit gene expression (Vousden and Prives, 2009). Currently, numerous lncRNAs are identified as the targets of p53, such as uc061hsf.1 (Yao et al, 2020), PiHL (Deng et al, 2020), and TRMP (Yang et al, 2018). Herein, two p53 binding motifs on ST8SIA6-AS1 promoter were predicted using in silico analysis, and we performed ChIP and luciferase reporter assays and found that p53 could bind to the motif away from the transcription initiation site, inhibiting ST8SIA6-AS1 expression.…”

Section: Discussionmentioning

confidence: 99%

Long Non-coding RNA ST8SIA6-AS1 Promotes Lung Adenocarcinoma Progression Through Sponging miR-125a-3p

Cao

Yang²,

et al. 2020

Front. Genet.

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Emerging evidence suggests that long non-coding RNA (lncRNA) plays a critical role in human disease progression. Recently, a novel lncRNA ST8SIA6-AS1 was shown as an important driver in various cancer types. Nevertheless, its contribution to lung adenocarcinoma (LUAD) remains undocumented. Herein, we found that ST8SIA6-AS1 was frequently overexpressed in LUAD cell lines, tissues, and plasma. Depletion of ST8SIA6-AS1 significantly inhibited LUAD cell proliferation and invasion in vitro and tumor growth in vivo. In term of mechanism, ST8SIA6-AS1 was transcriptionally repressed by tumor suppressor p53, and ST8SIA6-AS1 was mainly located in the cytoplasm and could abundantly sponge miR-125a-3p to increase nicotinamide N-methyltransferase (NNMT) expression, thereby facilitating LUAD malignant progression. Clinically, high ST8SIA6-AS1 was positively correlated with larger tumor size, lymph node metastasis, and later TNM stage. Moreover, ST8SIA6-AS1 was identified as an excellent indicator for MM diagnosis and prognosis. Collectively, our data demonstrate that ST8SIA6-AS1 is a carcinogenic lncRNA in LUAD, and targeting the axis of ST8SIA6-AS1/miR-125a-3p/NNMT may be a promising treatment for LUAD patients.

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Section: Discussionmentioning

confidence: 99%

Long Non-coding RNA ST8SIA6-AS1 Promotes Lung Adenocarcinoma Progression Through Sponging miR-125a-3p

Cao

Yang²,

et al. 2020

Front. Genet.

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“…More importantly, we identified that PKM2, a new target gene of miR-1294, played key roles in miR-1294mediated actions in osteosarcoma cells. Moreover, the tumor-suppressing role of miR-1294 has been reported in other of tumors, such as gastric cancer, 8 esophageal squamous cell carcinoma, 19 epithelial ovarian cancer, 7 oral squamous cell carcinoma, 9 and glioma. 11 Uncontrolled growth, invasion, and metastasis are the main characteristics of tumor cells.…”

Section: Discussionmentioning

confidence: 96%

“…Cell proliferation was determined by CCK-8 assay. 19 In brief, cells were seeded into 96-well plates at 4×10 3 cells per well. After culture for 0, 24, 48, 72, or 96 h at 37°C in 5% CO 2 , the medium was discarded and replaced with 100 μL complete medium and 10 μL CCK-8 (Sigma, St. Louis, MO, USA).…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

<p>Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells</p>

Yuan¹,

Yu²,

Chen³

et al. 2020

OTT

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Background: MicroRNAs (miRNAs) can act as negative regulators of gene expression, and play a crucial role in cancer progression. The aim of this study was to investigate the role of miR-1294/pyruvate kinase M2 (PKM2) axis in osteosarcoma cells in vitro and in vivo. Methods: The function of miR-1294 and its association with PKM2 in osteosarcoma cells were studied by real-time PCR, CCK-8, Western blot, scratch test, transwell assay, flow cytometry, and dual-luciferase reporter assays. The effect of miR-1294 on tumor growth in vivo was evaluated in a subcutaneous xenograft model of osteosarcoma. Results: miR-1294 was downregulated in osteosarcoma cells. Forced overexpression of miR-1294 inhibited cell proliferation, migration, and invasion, and induced G0/G1 arrest and apoptosis. Consistently, protein expression levels of proliferating cell nuclear antigen, c-Myc, cyclin D1, active matrix metalloproteinase 2, and active matrix metalloproteinase 9 were decreased, and cleaved caspase 3 and cleaved PARP were increased following miR-1294 overexpression. Moreover, we demonstrated that PKM2 was a target of miR-1294 in osteosarcoma cells, and the effects caused by miR-1294 mimic were reversed by the overexpression of PKM2. Furthermore, we found that upregulation of miR-1294 inhibited tumorigenesis of osteosarcoma cells in vivo, which was accompanied by downregulation of PKM2. Conclusion: Our results revealed that miR-1294/PKM2 signaling cascade exerts important roles in the regulation of tumor progression, implying that this pathway may serve as a potential therapeutic target in osteosarcoma.

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“…uc061hsf.1 is a direct transcription target of p53. uc061hsf.1 regulates the expression of the downstream transcription factor FoxA1 and inhibits the proliferation and migration of ESCC cells, indicating that uc061hsf.1 is a tumor suppressor LncRNA regulated by P53 ( 61 ).…”

Section: Long-chain Non-coding Rnas Inhibit the Occurrence And Develo...mentioning

confidence: 99%

Long Non-Coding RNA in Esophageal Cancer: A Review of Research Progress

Yang¹,

Chen²

2022

Pathol. Oncol. Res.

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In recent years, there has been significant progress in the diagnosis and treatment of esophageal cancer. However, owing to the lack of early diagnosis strategies and treatment targets, the prognosis of patients with esophageal cancer remains unsatisfactory. There is an urgent need to identify novel biomarkers and treatment targets for esophageal cancer. With the development of genomics, long-chain non-coding RNAs (LncRNAs), which were once considered transcriptional “noise,” are being identified and characterized rapidly in large numbers. Recent research shows that LncRNAs are closely related to a series of steps in tumor development and play an important regulatory role in DNA replication, transcription, and post-transcriptional regulation. The abnormal expression of LncRNAs leads to tumor cell proliferation, migration, invasion, and treatment resistance. This review focuses on the latest progress in research on the abnormal expression and functional mechanisms of LncRNAs in esophageal cancer. Further, it discusses the potential applications of these findings towards achieving an early diagnosis, improving treatment efficacy, and evaluating the prognosis of esophageal cancer.

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P53‐regulated lncRNA uc061hsf.1 inhibits cell proliferation and metastasis in human esophageal squamous cell cancer

Cited by 11 publications

References 33 publications

Long Non-coding RNA ST8SIA6-AS1 Promotes Lung Adenocarcinoma Progression Through Sponging miR-125a-3p

Long Non-coding RNA ST8SIA6-AS1 Promotes Lung Adenocarcinoma Progression Through Sponging miR-125a-3p

<p>Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells</p>

Long Non-Coding RNA in Esophageal Cancer: A Review of Research Progress

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