P60
Permeability of gloves to plant allergens

Abstract

To understand the mechanisms involved in immunological tolerance to skin-associated proteins, we have developed trangenic (Tg) mice that express a model self antigen, membrane-bound ABSTRACTS 125 FS01.3 Disperse (yes), orange (yes), 3 (no): what do we test in textile dye dermatitis?Para-phenylenediamine (PPD), an arylamine dye, is a strong allergen causing allergic contact dermatitis. Cytokines such as TNF-a and IL-1beta are key mediators in the initiation of this reaction. Both cytokines are predominantly produced by stimulated monocytes and macroghages. We investigated the responses of PPD and Bandrowski's base (BB), an autoxidation product of PPD in human monocytes. We isolated monocytes from healthy volunteers and incubated them with the allergens. TNF-a and IL-1beta mRNA expression and protein levels were estimated after 45 min, 2 h, 4 h and 24 h after allergen contact. IL-1beta and TNF-alpha were measured in cell culture supernatants by ELISA (n ¼ 7) and mRNA expression was determined by real-time RT-PCR. We found that PPD reduced TNF-a protein secretion by 20-69.9% (n ¼ 6). Further, IL-1beta levels were decreased by 44-98%. The same tendency was found studying IL-1beta and TNF-a mRNA steady state levels (n ¼ 3; 1 h incubation). These effects were substance-specific and not found for PPD derivatives nor for the autoxidation product BB. These findings suggest that PPD may specifically modify immune responses by directly infering with the cellular proinflammatory cytokine network.

Natural rubber gloves might not protect against skin penetration of methylisothiazolinone

“…In vivo studies using patch testing with a 48‐hour exposure have been used for some photographic chemicals , acrylate resin systems , and plant allergens, such as daily disulfide, Allium sativa , and α‐methylene‐γ‐butyrolactone. It was concluded that, although the 48‐hour exposure time is higher than that in real life, the results provided interesting data on glove permeability .…”

Parthenium the terminator: An update

“…[ 70 ] Gloves may not offer protection since the SQL permeates vinyl, polyethylene and latex gloves. [ 71 ] …”

Plants and Plant Products

“…A recent study shows that vinyl, polyethylene and latex gloves are likely permeable to plant allergens such as α-methylene-γ-butyrolactone, primin and diallyldisulfide. Nitrile gloves could protect from primin [383].…”