P66Shc mediated ferritin degradation—A novel mechanism of ROS formation

Borkowska, Andzelika; Sielicka-Dudzin, Alicja; Herman-Antosiewicz, Anna; Halon, Malgorzata; Woźniak, Michał; Antosiewicz, Jędrzej

doi:10.1016/j.freeradbiomed.2011.04.045

Cited by 33 publications

(34 citation statements)

References 30 publications

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“…In addition to that, we observed that in prostate cancer cells ferritin degradation was mediated by the adaptor protein p66Shc phosphorylated at serine 36. In this study, we proved that DATSinduced ROS formation, ferritin degradation, and increased LIP depended on p66Shc phosphorylation (7).…”

Section: Introductionsupporting

confidence: 52%

“…We have recently demonstrated that p66Shc mediates DATS-induced ferritin degradation. Ferritin degradation was almost completely abrogated in cells expressing plasmid encoding the inactive form of p66Shc (p66Shc S36A) (7). These data indicate that p66Shc phosphorylated at serine 36 in PC-3 cells leads to ferritin degradation (7).…”

Section: Discussionmentioning

confidence: 81%

“…We demonstrated that DATS treatment led to JNK-dependent phosphorylation and activation of p66Shc, and ligase Itch. The activation of this signaling pathway leads to ferritin degradation, an increase in LIP and iron-dependent ROS formation (6,7). Therefore one of the goals of this study was to evaluate if such signaling pathways would be activated in noncancerous prostate cell line after DATS treatment.…”

Section: Discussionmentioning

confidence: 99%

“…3B). In PC-3 cell line, we demonstrated that p66Shc phosphorylation induced by DATS was responsible for ferritin degradation (7). Therefore, the next goal of our study was to evaluate if DATS caused changes in ferritin level in PNT1A cells.…”

Section: Dats-induced P66shc Phosphorylation and Ferritin Degradationmentioning

confidence: 95%

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Diallyl Trisulfide Is More Cytotoxic to Prostate Cancer Cells PC-3 than to Noncancerous Epithelial Cell Line PNT1A: A Possible Role of p66Shc signaling Axis

Borkowska

Knap

Antosiewicz

2013

Nutrition and Cancer

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Diallyl trisulfide (DATS) is an organosulfur compound isolated from garlic, and has been shown to have anticancer activity both in vitro and in vivo. The aim of this study was to compare cytotoxic effects of DATS on prostate cancer cells PC-3 and noncancerous human prostate epithelial cells PNT1A. PC-3 prostate cancer and noncancerous human prostate epithelial cells PNT1A were used in the study. We observed that PNT1A cells had higher resistance to DATS-induced cell death than PC-3 cells. Investigating signaling pathways involved in the cell death we observed that p66Shc phosphorylation at serine 36 and extracellular signal-regulated kinase 1/2 activation induced by DATS, were significantly attenuated in PNT1A cells as compared to PC-3 cells. Moreover, DATS-induced Akt inactivation was also significantly reduced in PNT1A cells. In addition to that, DATS-induced reactive oxygen species generation was nearly completely abolished in PNT1A cells. Interestingly, DATS induced only slight decrease in the level of ferritin H, whereas ferritin L was elevated. These data suggest that cytotoxicity of DATS toward PNT1A cells is strongly reduced as opposed to PC-3 cancer cells, which corresponds to the lower activation of prodeath signaling pathway mediated by the adaptor protein p66Shc in the noncancerous PNT1A cells.

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Section: Introductionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Dats-induced P66shc Phosphorylation and Ferritin Degradationmentioning

confidence: 95%

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Diallyl Trisulfide Is More Cytotoxic to Prostate Cancer Cells PC-3 than to Noncancerous Epithelial Cell Line PNT1A: A Possible Role of p66Shc signaling Axis

Borkowska

Knap

Antosiewicz

2013

Nutrition and Cancer

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“…1A). In different cells, extracellular iron catalyzes the production of ROS through the Fenton reaction (Borkowska et al, 2011); therefore, we evaluated the effect of sodium nitroprusside on ROS production in spermatozoa using fluorescent dye assays. Significant increases in ROS production were observed in spermatozoa treated with all concentrations of sodium nitroprusside compared with the control (p = 0.001) (Fig.…”

Section: The Effect Of Sodium Nitroprusside On the Intracellular Ironmentioning

confidence: 99%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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SUMMARYSodium nitroprusside is a nitric oxide donor involved in the regulation of the motility, hyperactivation, capacitation, and acrosome reaction (AR) of spermatozoa. However, the molecular mechanism underlying this regulation has not yet been elucidated. Therefore, this study was designed to evaluate the molecular basis for the effects of sodium nitroprusside on different processes in spermatozoa and its consequences on subsequent oocyte fertilization and embryo development. In this in vitro study, mouse spermatozoa were incubated with various concentrations of sodium nitroprusside (1, 10, and 100 lM) for 90 min. Our results showed that sodium nitroprusside inhibited sperm motility and motion kinematics in a dose-dependent manner by significantly enhancing intracellular iron and reactive oxygen species (ROS), and decreasing Ca 2+ , and adenosine triphosphate levels in spermatozoa. Moreover, short-term exposure of spermatozoa to sodium nitroprusside increased the tyrosine phosphorylation of sperm proteins involved in PKA-dependent regulation of intracellular calcium levels, which induced a robust AR. Finally, sodium nitroprusside significantly decreased the rates of fertilization and blastocyst formation during embryo development. Based on these results, we propose that sodium nitroprusside increases ROS production and precocious AR may alter overall sperm physiology, leading to poor fertilization and compromised embryonic development.

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NOX1, Reactive Oxygen Species, JNK, and Necrotic Cell Death

Morgan

Kim

2014

Necrotic Cell Death

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P66Shc mediated ferritin degradation—A novel mechanism of ROS formation

Cited by 33 publications

References 30 publications

Diallyl Trisulfide Is More Cytotoxic to Prostate Cancer Cells PC-3 than to Noncancerous Epithelial Cell Line PNT1A: A Possible Role of p66Shc signaling Axis

Diallyl Trisulfide Is More Cytotoxic to Prostate Cancer Cells PC-3 than to Noncancerous Epithelial Cell Line PNT1A: A Possible Role of p66Shc signaling Axis

Sodium nitroprusside suppresses male fertility in vitro

NOX1, Reactive Oxygen Species, JNK, and Necrotic Cell Death

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