PacBio Full-Length Transcriptome Sequencing Reveals the Mechanism of Salt Stress Response in Sonneratia apetala

Abstract

Sonneratia apetala is an essential mangrove wetland restoration tree species. Studying its molecular mechanism for salt tolerance could lay a foundation for further cultivating excellent resistant germplasm. This study used a combination of PacBio isoform sequencing (Iso-seq) and BGISEQ RNA sequencing (RNA-seq) to analyze the molecular mechanism to salt stress response of one-year-old S. apetala leaves. The growth and physiological analysis showed that physiological indexes such as growth rate, net photosynthetic rate and antioxidant enzyme activity all exhibit significant changes under salt stress. From Iso-seq, a total of 295,501 full-length transcripts, with an average length of 1418 bp, were obtained. RNA-seq produced 4712 differentially expressed genes (DEGs) as compared to a control group. Of these, 930 were identified to be co-expressed during the STEM time sequence analysis. Further, 715 and 444 co-expressed DEGs were annotated by GO and KEGG analyses, respectively. Moreover, 318 of the co-expressed DEGs were annotated as essential genes that were implicated in salt stress response of S. apetala, which were involved in transcription factors, signal transduction, hormone response, ROS homeostasis, osmotic balance, cell wall synthesis or modification. These results provide candidate targets for further characterization and offer insights into the salt-tolerant mechanism of S. apetala.

Phenomenon and Mechanisms of Sonneratia apetala Introduction and Spread Promoting Excessive Growth of Derris trifoliata

“…For example, in areas lacking rivers, certain dynamics may not be as apparent [28]. Additionally, owing to the limited duration of this study, we may not have captured changes in long-term ecological processes, such as mudflat elevation [29], soil nutrient accumulation [30][31][32][33], allelopathy [34], light impact [35,36], adaptability to salinity [37][38][39], natural enemy factors [40,41], gene differentiation [42], etc. Hence, further research should encompass a broader geographical scope and longer time scales to obtain a more comprehensive understanding.…”