Pacinian corpuscle development involves multiple Trk signaling pathways

Šedý, Jiří; Szeder,; Jm, Walro; Zg, Ren; Naňka, Ondřej; Tessarollo, Lino; Sieber‐Blum, Maya; Grim, Miloš; Kučera, Jan

doi:10.1002/dvdy.20156

Cited by 33 publications

(6 citation statements)

References 54 publications

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“…Pacinian corpuscles and Ruffini endings, typically identifiable with hematoxylin and eosin staining alone, were not observed despite close examination along the phalange, metatarsal, and tarsal bones of the hind paw. Previous histological investigations have confirmed the absence of Ruffini-like structures in the forepaw digit of the raccoon (Rice and Rasmusson, 2000) and have identified Pacinian corpuscles in forelimb phalanges, radius, ulna, fibula, tibia, and interosseous membranes of mice (Zelená, 1978;Sedý et al, 2004;Prsa et al, 2019), but not in the hind paw. Though, we cannot discount that our sampling approach [every third slide (88 µm)] may have missed these receptors that are few in number and ∼50 µm in diameter (Prsa et al, 2019), it would make sense functionally that feedback regarding the high-frequency vibrations of phalanges in the forepaw may be critical for feeding and grooming, but not in the hind paw for sitting and gait (Hunt, 1961).…”

Section: Discussionmentioning

confidence: 88%

The Anatomical Distribution of Mechanoreceptors in Mouse Hind Paw Skin and the Influence of Integrin α1β1 on Meissner-Like Corpuscle Density in the Footpads

et al. 2021

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Afferent neurons and their mechanoreceptors provide critical sensory feedback for gait. The anatomical distribution and density of afferents and mechanoreceptors influence sensory feedback, as does mechanoreceptor function. Electrophysiological studies of hind paw skin reveal the different types of afferent responses and their receptive fields, however, the anatomical distribution of mechanoreceptor endings is unknown. Also, the role of integrin α1β1 in mechanoreceptor function is unclear, though it is expressed by keratinocytes in the stratum basale where it is likely involved in a variety of mechanotransduction pathways and ion channel functionalities. For example, it has been shown that integrin α1β1 is necessary for the function of TRPV4 that is highly expressed by afferent units. The purpose of this study, therefore, was to determine and compare the distribution of mechanoreceptors across the hind paw skin and the footfall patterns of itga1-null and wild type mice. The itga1-null mouse is lacking the integrin α1 subunit, which binds exclusively to the β1 subunit, thus rendering integrin α1β1 nonfunctional while leaving the numerous other pairings of the β1 subunit undisturbed. Intact hind paws were processed, serially sectioned, and stained to visualize mechanoreceptors. Footfall patterns were analyzed as a first step in correlating mechanoreceptor distribution and functionality. Merkel cells and Meissner-like corpuscles were present, however, Ruffini endings and Pacinian corpuscles were not observed. Meissner-like corpuscles were located exclusively in the glabrous skin of the footpads and digit tips, however, Merkel cells were found throughout hairy and glabrous skin. The increased density of Merkel cells and Meissner-like corpuscles in footpads 1 and 3 and Meissner-like corpuscles in footpad 4 suggests their role in anteroposterior balance, while Meissner-like corpuscle concentrations in digits 2 and 5 support their role in mediolateral balance. Finally, a larger density of Meissner-like corpuscles in footpads 3 and 4 in male itga1-null mice compared to wild type controls paves the way for future site-specific single fiber in vivo recordings to provide insight into the role of integrin α1β1 in tactile mechanotransduction.

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Section: Discussionmentioning

confidence: 88%

The Anatomical Distribution of Mechanoreceptors in Mouse Hind Paw Skin and the Influence of Integrin α1β1 on Meissner-Like Corpuscle Density in the Footpads

et al. 2021

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“…The DRG markers consist of NF-200 (mechanoreceptors and proprioreceptors with large axon diameters), parvalbumin (proprioceptors), TrkA (small and medium diameter unmyelinated nociceptors and mechanoreceptors), CGRP (peptidergic nociceptors), IB4 (nonpeptidergic nociceptors), and peripherin (nociceptors and mechanoreceptors). TrkA is expressed in and required for the development of both peptidergic nociceptors (Marmigère and Ernfors, 2007) and a variety of mechanoreceptors (Fundin et al, 1997b; Cronk et al, 2002; Sedý et al, 2004). …”

Section: Resultsmentioning

confidence: 99%

Combinatorial Expression of Brn3 Transcription Factors in Somatosensory Neurons: Genetic and Morphologic Analysis

et al. 2012

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The three members of the Brn3 family of POU-domain transcription factors (Brn3a/Pou4f1, Brn3b/Pou4f2, and Brn3c/Pou4f3) are expressed in overlapping subsets of visual, auditory/vestibular, and somatosensory neurons. Using unmarked Brn3 null alleles and Brn3 conditional alleles in which gene loss is coupled to expression of an alkaline phosphatase reporter, together with sparse Cre-mediated recombination, we describe (1) the overlapping patterns of Brn3 gene expression in somatosensory neurons, (2) the manner in which these patterns correlate with molecular markers, peripheral afferent arbor morphologies, and dorsal horn projections, and (3) the consequences for these neurons of deleting individual Brn3 genes in the mouse. We observe broad expression of Brn3a among DRG neurons, but subtype-restricted expression of Brn3b and Brn3c. We also observe a nearly complete loss of hair follicle-associated sensory endings among Brn3a−/− neurons. Together with earlier analyses of Brn3 gene expression patterns in the retina and inner ear, these experiments suggest a deep functional similarity between primary somatosensory neurons, spiral and vestibular ganglion neurons, and retinal ganglion cells. This work also demonstrates the utility of sparse genetically-directed labeling for visualizing individual somatosensory afferent arbors and for defining cell-autonomous mutant phenotypes.

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“…The firing of Pacinians across the step cycle led us to ask how mechanical stimuli move through the body. In the hindlimb, Pacinians are most often found at the base of the fibula 9,32 , suggesting that vibration propagates through the bone and tissue of the ankle to activate Pacinians. Could footfalls from the contralateral foot propagate through the body’s skeletal system to activate Pacinians?…”

Section: Main Textmentioning

confidence: 99%

“…These externally generated stimuli were often How do vibrations propagate through the mouse to activate Pacinians? In the hindlimb, Pacinians are most often found at the base of the fibula 42,43 , suggesting that vibration propagates through the bone and tissue of the ankle to activate Pacinians. Given their sensitivity to both internally generated movement and upper body movements, we asked whether hindlimb Pacinians could also detect vibrations that propagate from either the contralateral hindlimb or the forelimbs.…”

Section: Detection Of Externally Generated Vibrationsmentioning

confidence: 99%

Coding of self and environment by Pacinian neurons in freely moving animals

Turecek,

Ginty

2023

Preprint

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Pacinian corpuscle neurons are specialized low-threshold mechanoreceptors (LTMRs) that are exquisitely tuned to high-frequency vibration (~40-1000 Hz). Although much is known about their tuning properties, it is unclear how Pacinians encode naturalistic behavior. Here, we developed a technique to record from Pacinian neurons and other LTMRs in awake, freely moving mice. We find that Pacinian LTMRs can robustly respond to self-generated vibrations caused by movement alone. Pacinians are highly active during a wide variety of natural behaviors, including grooming, digging, and climbing. Pacinians in the hindlimb are sensitive enough to be activated by forelimb- or upper-body-dominant behaviors. Strikingly, Pacinians can uniquely and reliably encode subtle vibrations of surfaces encountered by the animal, including low-amplitude vibrations initiated over two meters away. Finally, as a population, there is a broad range of Pacinian responses during natural behaviors and environmental vibrations, and an individual Pacinians responsiveness is strongly dependent on its distinct sensitivity and frequency tuning. Thus, Pacinian corpuscle LTMRs, with their varying sensitivities, frequency tuning, and body distributions, collectively encode a wealth of vibro-tactile features of self-initiated movements and the environment.

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Pacinian corpuscle development involves multiple Trk signaling pathways

Cited by 33 publications

References 54 publications

The Anatomical Distribution of Mechanoreceptors in Mouse Hind Paw Skin and the Influence of Integrin α1β1 on Meissner-Like Corpuscle Density in the Footpads

The Anatomical Distribution of Mechanoreceptors in Mouse Hind Paw Skin and the Influence of Integrin α1β1 on Meissner-Like Corpuscle Density in the Footpads

Combinatorial Expression of Brn3 Transcription Factors in Somatosensory Neurons: Genetic and Morphologic Analysis

Coding of self and environment by Pacinian neurons in freely moving animals

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