Package coating with hydrosorbent products and the shelf-life of cheeses

Mathlouthi, Mohamed; Leiris, J. P. de; Seuvre, Anne-Marie

doi:10.1007/978-1-4615-2173-0_6

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…During cooling, the glycosidic band (995 cm –1 ) gradually shifted to higher wavenumbers, experiencing an abrupt drop around −30 °C, which corresponded to the freezing of the sample. The blueshift of glycosidic linkage peak location with decreasing temperature is due to the increase in hydration level for trehalose. , More water–trehalose HBs form at lower temperatures, decreasing the amount of trehalose–trehalose intermolecular HBs (note that trehalose does not form intramolecular HBs), , also increasing the flexibility of trehalose around the glycosidic linkage . On the other hand, the abrupt redshift during freezing was because of the formation of the freeze-concentrated liquid (FCL).…”

Section: Resultsmentioning

confidence: 99%

Physicochemical Mechanisms of Protection Offered by Agarose Encapsulation during Cryopreservation of Mammalian Cells in the Absence of Membrane-Penetrating Cryoprotectants

Wang

Mahajan

Miller

et al. 2023

ACS Appl. Bio Mater.

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During freeze/thaw, cells are exposed to mechanical, thermal, chemical, and osmotic stresses, which cause loss of viability and function. Cryopreservation agents such as dimethyl sulfoxide (DMSO) are deployed to minimize freeze/thaw damage. However, there is a pressing need to eliminate DMSO from cryopreservation solutions due to its adverse effects. This is of the highest priority especially for cryopreservation of infusible/transplantable cell therapy products. In order to address this issue, we introduce reversible encapsulation in agarose hydrogels in the presence of the membraneimpermeable cryoprotectant, trehalose, as a viable, safe, and effective cryopreservation method. Our findings, which are supported by IR spectroscopy and differential scanning calorimetry analyses, demonstrate that encapsulation in 0.75% agarose hydrogels containing 10−20% trehalose inhibits mechanical damage induced by eutectic phase change, devitrification, and recrystallization, resulting in post-thaw viability comparable to the gold standard 10% DMSO.

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