“…The emitted light of longer wavelength re-radiates spherically in all directions, regardless of the excitation light source direction. If the specimen fluoresces, the emitted light gathered by the objective lens passes back through the dichromatic mirror and is subsequently filtered by a barrier (emission) filter, which blocks the unwanted excitation wavelength [9,10]. After leaving the microscope, the light enters a detector, which in the present case is a spectrometer.…”