Paclitaxel-loaded ethosomes®: Potential treatment of squamous cell carcinoma, a malignant transformation of actinic keratoses

Paolino, Donatella; Celia, Christian; Trapasso, Elena; Cilurzo, Felisa; Fresta, Massimo

doi:10.1016/j.ejpb.2012.02.008

Cited by 109 publications

(55 citation statements)

References 42 publications

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“…Higher EE with increased amount of ethanol is possibly due to increased solubility of B. aristata extract in ethanol present in the ethosomal core. This is in accordance with the previous finding by Paolino et al, 2012. [21] On the basis of small vesicle size, uniform size distribution, and higher EE batch F 12 was selected for in vitro diffusion studies [ Figure 1a and b] and further incorporated into gel formulation (GELF12) for in vivo studies.…”

Section: Resultssupporting

confidence: 73%

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Fatima

2017

AJP

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Aims: The aim of the present research work is to prepare ethosome of standardized Berberis aristata extract and to evaluate for vesicle shape, size, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency (EE), and in vitro permeation studies. Materials and Methods: Ethosomes were prepared using soyaphosphatidylcholine (1-3%) and ethanol (10-40%) by modified cold method and were characterized. Results: The size of ethosomes were found to be in the range of 110.98-357.34 nm while PDI ranges from 0.114 to 1.56 and ZP was between −20.0 and −39.4 mV. Morphology studies showed unilamellar structure under transmission electron microscope and phase contrast microscope showed smooth surface. The EE of ethosomes was found to be in the range of 48.05% to 92.08%. Ethosomes were further added to carbopol 934P for gel formation, and subsequently, evaluated for their physicochemical properties. Discussion: In vitro diffusion study was conducted for ethosomal dispersion, hydroethanolic solution, ethosomes incorporated in viscous gel, and conventional gel using Franz diffusion cell for the biomarker compound berberine. Conclusions: It can be concluded that B. aristata loaded ethosomal delivery system is an encouraging approach for herbal drugs.

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Section: Resultssupporting

confidence: 73%

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Fatima

2017

AJP

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“…31 Transmission electron microscopy was performed according to a procedure described elsewhere. 32 The amount of pCMV-GFP encapsulated in the nanoparticles was spectrophotometrically determined.…”

Section: Physicochemical Characterization and In Vitro Releasementioning

confidence: 99%

Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Cosco

Federico

Maiuolo

et al. 2014

IJN

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The aim of this study was the evaluation of the effects of two emulsifiers on the physicochemical and technological properties of low molecular weight chitosan/poly (D,L-lactide-co-glycolide) (PLGA) nanoplexes and their transfection efficiency. Nanospheres were prepared using the nanoprecipitation method of the preformed polymer. The mean diameter and surface charge of the nanospheres were investigated by photocorrelation spectroscopy. The degree of binding of the plasmid with the nanoplexes was qualitatively and quantitatively determined. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) testing was performed using HeLa, RPMI8226, and SKMM1 cell lines. Flow cytometry and confocal laser scanning microscopy were used to determine the degree of cellular transfection and internalization of the nanoplexes into cells, respectively. The nanoplexes had a positive zeta potential, and low amounts of PLGA and poloxamer 188 showed a mean colloidal size of ~200 nm with a polydispersity index of ~0.14. The nanoplexes had suitable entrapment efficiency (80%). In vitro experiments showed that the colloidal nanodevices did not induce significant cytotoxicity. The nanoplexes investigated in this study could represent efficient and useful nonviral devices for gene delivery. Use of low amounts of PLGA and poloxamer 188 enabled development of a nanosphere able to transfect cells efficiently. These nanosystems are a helpful platform for delivery of genetic material while preserving therapeutic efficacy.

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“…However, the permeation enhancement from ethosomes was much greater than would be expected from ethanol alone, suggesting some kind of synergistic mechanism between ethanol, vesicles and skin lipids providing flexible characteristics to ethosomes generating enhanced penetration abilities which is attributed to two effects (a) increase in thermodynamic activity due to evaporation of ethanol known as ''push effect'' and (b) pull effect in which penetration of drug molecule is increased due to reduction in barrier property of subcutaneous tissue by ethanol (Kadir et al, 1987;Panchagnula et al, 2001;Dubey et al, 2007b). Paolino et al (2012) showed percutaneous flux of $8.2 ± 0.32 (mg/cm 2 h) for Paclitaxel loaded ethosomes which was $3.2-fold higher than that observed for the Paclitaxel contained in the physical mixture (paclitaxel and empty ethosomes) (2.62 ± 0.18 mg/cm 2 h) and $23.2-fold higher than that observed for the paclitaxel hydroalcoholic suspension (0.37 ± 0.01 mg/cm 2 h; Paolino et al, 2012).…”

Section: Permeation Distinctivenessmentioning

confidence: 99%

Ethosomes: versatile vesicular carriers for efficient transdermal delivery of therapeutic agents

Pandey¹,

Golhani²,

Shukla³

2014

Drug Delivery

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Delivery across skin is attractive due to its easy accessibility. However, drug delivery across skin is still a challenge in biomedical sciences. Over the past few decades, various successful novel devices and techniques have emerged to optimize drug delivery across skin whose obstructing behavior constricts entry of most of the therapeutic agents. Inability of various conventional vesicular formulations, e.g. liposomes to pass through the tapered (430 nm) intercellular channels of stratum corneum, rendered invention of some lipid based vesicular carrier systems such as ethosomes which consist of phospholipid, ethanol and water. Ethosomes are noninvasive delivery carriers that enable drugs to reach the deep skin layers and/or the systemic circulation. In spite of their sophistication in conceptuality, they are exemplified by easiness in their preparation, safety and efficacy -a combination that can highly inflate their application. This review attempts to describe all aspects of ethosomes including roles and upshots of different excipients, various methods of preparation and characterizations, research reports on various drug deliveries, patent reports and future prospects.

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Paclitaxel-loaded ethosomes®: Potential treatment of squamous cell carcinoma, a malignant transformation of actinic keratoses

Cited by 109 publications

References 42 publications

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Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Ethosomes: versatile vesicular carriers for efficient transdermal delivery of therapeutic agents

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