Paclitaxel production is enhanced in suspension‐cultured hazel (Corylus avellana L.) cells by using a combination of sugar, precursor, and elicitor

Abstract

Hazel (Corylus avellana L.) has recently been drawing attention as an alternative source of taxol. In the present study, the effects of sugar type, and different concentrations of phenylalanine (Phe) and vanadyl sulfate (V) on the production of taxol in C. avellana were investigated. A factorial experiment was used to optimize the concentrations of the precursor and elicitor. The cells were treated with Phe and V on the fourth day of culture and were harvested every 2 days until the 10th day. By increasing the Phe and V supply, taxol production increased during the culture period and the maximum level of 4.2 μg/g (dry weight) was obtained at day 10 by combining 3 μM of Phe and 0.05 and 0.1 mM of V in media supplemented with fructose (3%). The time course study on taxol production suggested that the appropriate time for using Phe is day 4 of culture, and day 8 for V. Overall, taxol production in C. avellana cell suspension culture was improved by the use of the combined strategy.

Fungal Cell Wall and Methyl-β–Cyclodextrin Synergistically Enhance Paclitaxel Biosynthesis and Secretion in Corylus avellana Cell Suspension Culture

“…Also, the addition of benzoic acid to C. avellana CSC resulted in a 4-fold in paclitaxel biosynthesis 56 . In another research, Rahpeyma et al 15 displayed that the joint effects of phenylalanine (3 μM) and vanadyl sulfate (0.05 and 0.1 mM) in culture medium completed with fructose (3% (v/v)) led to a 2.3-fold increment in paclitaxel biosynthesis.…”

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

“…Calli cultures are often used successfully to produce secondary metabolites of medical significance, many of which may be used in treating human diseases [50][51][52][53]. The synthesis of the desired compound can be increased by designing the genetic constructs and the way they are introduced.…”

Plant cell culture as emerging technology for production of active cosmetic ingredients

“…Because plant cells are considered totipotent, they have the potential to express the full genetic machinery coded in the nucleus, and thus, they are able to produce the full spectrum of characteristic secondary metabolites, found in mother plants. This is very important advantage especially for the production of rare bioactive compounds, as resveratrol, paclitaxel or terpenoids, which are usually found in low concentrations in plants and their isolation and purification requires the processing of large amounts plant biomass [21][22][23][24][25]. Moreover, in vitro cultured plant cells are characterized with fast growth, and the ability to accumulate large amount of uniform biomass for a short period of time [19,20].…”

“…Moreover, in vitro cultured plant cells are characterized with fast growth, and the ability to accumulate large amount of uniform biomass for a short period of time [19,20]. This is very important advantage especially for the production of rare bioactive compounds, as resveratrol, paclitaxel or terpenoids, which are usually found in low concentrations in plants and their isolation and purification requires the processing of large amounts plant biomass [21][22][23][24][25]. Additionally, plant cell culture technology offers a reliable and powerful production platform for continuous supply of contamination-free, phytochemically uniform biomass from herbal, aromatic, medicinal, and even from rare and threatened plant species [26].…”