Paclobutrazol mediated changes in growth and physio-biochemical traits of okra (Abelmoschus esculentus L.) grown under drought stress

Iqbal, Samreen; Parveen, Nusrat; Bahadur, Saraj; Ahmad, Tanveer; Shuaib, Muhammad; Nizamani, Mir Muhammad; Urooj, Zara; Rubab, Saima

doi:10.1016/j.genrep.2020.100908

Cited by 13 publications

(18 citation statements)

References 45 publications

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“…These compounds protect the plants from harmful conditions such as stress, pollution, UV exposure, pathogenic attack and drought. Recent research has shown that the significant intake of phytochemicals protects the human body from different diseases (Iqbal et al, 2020; Kamalarajan, Muthuraman, Ganesh, & Valan, 2019). Different types of phytochemicals have been reported showing antimicrobial activity but still, their therapeutic potential has not been well‐studied (Ashfaq et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Chemo‐taxonomic and biological potential of highly therapeutic plant Pedicularis groenlandica Retz. using multiple microscopic techniques

Hameed

Zafar

Ahmad

et al. 2021

Microscopy Res & Technique

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Pedicularis groenlandica is one of the most important medicinal plant of Deosai Plateau (Gilgit-Baltistan) Pakistan. The present study was aim to evaluate the micromorphological features, phytochemical screening and pharmacological potential of P. groenlandica by using multiple microscopic techniques. Six different solvents were used to prepare P. groenlandica extracts. Phytochemical and antioxidant activities were determined calorimetrically. To investigate antidiabetic, α-amylase inhibition assay was performed. Cytotoxicity was tested using brine shrimp assay.Anti-leishmanial via MTT assay. Disc-diffusion assay was used for protein kinase inhibitory, antibacterial and antifungal activities. Palyno-anatomical study showed significant variation for the authentication and correct identification of this highly therapeutic plant by using light and scanning electron microscopic techniques. All extracts were found rich in phytochemicals, significant amount of phenolic and flavonoid contents were found in methanol extract (PGM) 95.78 mg GAE/g and 66.90 mg QE/g. Highest DPPH scavenging potential with IC 50 88.65 μg/mL, total antioxidant capacity (60.33 mg AAE/g sample) and total reducing power (83.97 mg AAE/g) were found for PGM. Disc-diffusion method showed significant antibacterial and antifungal activities. Noticeable growth inhibition in L. tropica was displayed by n-hexane extract (IC 50 112 μg/mL). Brine shrimp with highest LD 50 (67.65 μg/mL) in ethyl-acetate extract. Ethanol extract gives persuasive protein kinase inhibition (26 mm) against Streptomyces 85-E hyphae. Highest alphaamylase inhibition (74.10%) was found in n-hexane extract. In conclusion, our findings scientifically support the ethno-medicinal and biological potential of P. groenlandica. In future, the plant needs to be explored for further identification and isolation of bioactive compounds to develop new drugs to treat several aliments.

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Section: Resultsmentioning

confidence: 99%

Chemo‐taxonomic and biological potential of highly therapeutic plant Pedicularis groenlandica Retz. using multiple microscopic techniques

Hameed

Zafar

Ahmad

et al. 2021

Microscopy Res & Technique

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“…Many studies published on the role of paclobutrazol in growing tomato seedlings, including applied different doses of PBZ (i.e., 50 and 100 mg L −1 ) through seed treatment or watering seedlings [ 28 ], production of tomato seedlings by applying PBZ (50, 100, and 150 mg L −1 ) using two tomato hybrids [ 29 ], and accelerating growth of tomato seedlings by applying 25, 50, and 100 mg L −1 PBZ [ 30 ]; however, to our knowledge, there are no studies on the crucial role of PBZ against early blight ( Alternaria solani ). On the other hand, many studies reported on the reduction of vegetative growth via applied PBZ on many cultivated crops, such as potato [ 31 ], mango [ 32 ], Leonotis leonurus L. [ 33 ], and olive [ 16 ], as well as its potential under stress, i.e., drought [ 13 , 14 , 34 ] and salinity [ 9 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…This study produced results that corroborate the findings of a great deal of previous work in promoting growth of plants under stress by applying PBZ. PBZ improves plant tolerance against different stresses by increasing proline content and enzymatic antioxidants [ 14 ], increases fruit yield (due to the relatively stouter canopy of PBZ-treated plants), improving rooting system (which may increase the uptake of water and nutrients) [ 36 ], regulating photosynthetic capacity and delaying leaf senescence [ 37 ], improving the resistance against many plant pathogens [ 38 ], and acting as a systemic fungicide against several economically fungal diseases [ 39 ]. The mode of action of paclobutrazol may include the inhibition of gibberellic acid synthesis in plants, which reduces gibberellins level, slows cell division and elongation (without causing toxicity to cells), and increases cytokinin content, as well as the root activity and C: N ratio.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies confirmed that applied PBZ improved various kinds of compatible solutes and osmo-protectants, such as proline, which increases the plant’s tolerance to water deficits [ 9 , 12 ]. Many benefits of PBZ application have been intensively reported, including improving crop productivity, plant stress tolerance, fruit/grain quality, plant water relation, and membrane stability index [ 13 , 14 ]. In addition, PBZ prevents sucker re-growth in bananas [ 15 ], promoting fruit sets in many crops (such as olives) [ 16 ], as well as inhibiting the biosynthesis of gibberellin, early fruit set, and reduced stem growth [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Paclobutrazol Improves the Quality of Tomato Seedlings to Be Resistant to Alternaria solani Blight Disease: Biochemical and Histological Perspectives

Shalaby

Taha

Taher

et al. 2022

Plants

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The production and quality of tomato seedlings needs many growth factors and production requirements besides controlling the phytopathogens. Paclobutrazol (PBZ) has benefit applications in improving crop productivity under biotic stress (Alternaria solani, the causal agent of early blight disease in tomatoes). In the current study, the foliar application of PBZ, at rates of 25, 50, and 100 mg L−1, was evaluated against early blight disease in tomatoes under greenhouse conditions. The roles of PBZ to extend tomato seedling lives and handling in nurseries were also investigated by measuring different the biochemical (leaf enzymes, including catalase and peroxidase) and histological attributes of tomato seedlings. Disease assessment confirmed that PBZ enhanced the quality of tomato seedlings and induced resistance to early blight disease post inoculation, at 7, 14, and 21 days. Higher values in chlorophyll content, enzyme activities, and anatomical features of stem (cuticle thickness) and stomata (numbers and thickness) were recorded, due to applied PBZ. This may support the delay of the transplanting of tomato seedlings without damage. The reason for this extending tomato seedling life may be due to the role of PBZ treatment in producing seedlings to be greener, more compact, and have a better root system. The most obvious finding to emerge from this study is that PBZ has a distinguished impact in ameliorating biotic stress, especially of the early blight disease under greenhouse conditions. Further studies, which consider molecular variables, will be conducted to explore the role of PBZ in more detail.

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“…Almeida et al (2015) reported that the formation of phenols, lignin, starch and gels in plant root and stem cells serve as physical and chemical barriers which constitute the activation of multiple defense mechanisms against M. phaseolina . Moreover this plant should be further screened out for its phytochemical, medicinal and pharmacological potential by following the previously published protocol (Bahadur et al, 2020; Ijaz, Durrani, Rubab, & Bahadur, 2021; Rubab et al, 2020, 2021), ecological (Sajad et al, 2020; Shuaib, Bahadur, & Hussain, 2020) antimicrobial and phytotoxic potential (Azam et al, 2020; Shuaib et al, 2021) nanoparticles (Liaqat et al, 2021) and physiological (Iqbal et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Effect of necrotrophic fungus and PGPR on the comparative histochemistry of Vigna radiata by using multiple microscopic techniques

Javed

Javaid

Hanif

et al. 2021

Microscopy Res & Technique

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Rapid advances in the field of pathogen detection have opened new opportunities and better understanding for their management approaches. Aim of this study was to elucidate histopathological observations of different tissues affected by Macrophomina phaseolina and to observe the defense responses of plant growth promoting rhizobacteria (PGPR) in mungbean plants. Sections of the stem and root were prepared and stained with ferric chloride, Lugol's iodine and Wiesner's reagent and were then observed under multiple microscopic techniques. Results revealed that both pathogen and PGPR produce responses on the plant that include colonization of xylem vessels by hyphae and sclerotia, hypertrophy and hyperplasia of the cells, destruction of xylem fibers and amyloplasts in parenchymatous cells; and production of gels by the plant were observed. There was a significant increase in lignin and phenolic compounds deposition in stem and root sections of PGPR treated and non‐treated mungbean plants. Whereas the soil amended with PGPR showed very less to no starch production. Moreover, production of gels and gums were also observed in both stem and root sections. Compared to light microscopy, scanning electron microscope provided greater depth of focus and resolution of the pathogen attack on plant tissues, associated bacteria. As a whole, the data demonstrated that inoculation of PGPR can be an effective strategy to stimulate plant growth and they could significantly activate disease resistance against M. phaseolina.

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Paclobutrazol mediated changes in growth and physio-biochemical traits of okra (Abelmoschus esculentus L.) grown under drought stress

Cited by 13 publications

References 45 publications

Chemo‐taxonomic and biological potential of highly therapeutic plant Pedicularis groenlandica Retz. using multiple microscopic techniques

Chemo‐taxonomic and biological potential of highly therapeutic plant Pedicularis groenlandica Retz. using multiple microscopic techniques

Paclobutrazol Improves the Quality of Tomato Seedlings to Be Resistant to Alternaria solani Blight Disease: Biochemical and Histological Perspectives

Effect of necrotrophic fungus and PGPR on the comparative histochemistry of Vigna radiata by using multiple microscopic techniques

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