Paclobutrazol on &amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; Growth and Development of &amp;lt;i&amp;gt;Zygopetalum crinitum&amp;lt;/i&amp;gt; Orchid, and on Seedling Acclimatization

Gimenes, Rossano; Pivetta, Káthia Fernandes Lopes; Mazzini-Guedes, Renata Bachin; Ferraz, Marcos Vieira; Pereira, Suzana Targanski Sajovic; Santos, Águila Silva; Faria, Ricardo Tadeu de; Almeida, Lívia Caroline Praseres de

doi:10.4236/ajps.2018.95079

Cited by 10 publications

(9 citation statements)

References 13 publications

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“…Among others, it is used to induce flowering, fruiting and prevent fungal infections ( Wang, Chang-Yi Wu & Lonameo, 2019 ). PBZ has many uses in in vitro culture for different plants, such as shoot multiplication medium MS + 5 mgL −1 in Phoenix dactylifera ( Awadh, Abdulhussein & Almusawi, 2019 ), rooting medium 1/2 B5 + 0.1 mgL −1 in N. cadamba ( Mok & Ho, 2019 ) and rooting medium MS + 1.5 mgL −1 in Zygopetalum crinitum ( Gimenes et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Eng

Ling³

2021

PeerJ

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Polyploidization has played a crucial role in plant breeding and crop improvement. However, studies on the polyploidization of tropical tree species are still very scarce in this region. This paper described the in vitro induction and identification of polyploid plants of Neolamarckia cadamba by colchicine treatment. N. cadamba belongs to the Rubiaceae family is a natural tetraploid plant with 44 chromosomes (2n = 4x = 44). Nodal segments were treated with colchicine (0.1%, 0.3% and 0.5%) for 24 h and 48 h before transferring to shoot regeneration medium. Flow cytometry (FCM) and chromosome count were employed to determine the ploidy level and chromosome number of the regenerants, respectively. Of 180 colchicine-treated nodal segments, 39, 14 and 22 were tetraploids, mixoploids and octoploids, respectively. The highest percentage of polyploidization (20% octoploids; 6.7% mixoploids) was observed after treated with 0.3% colchicine for 48 h. The DNA content of tetraploid (4C) and octoploid (8C) was 2.59 ± 0.09 pg and 5.35 ± 0.24 pg, respectively. Mixoploid plants are made up of mixed tetraploid and octoploid cells. Chromosome count confirmed that tetraploid cell has 44 chromosomes and colchicine-induced octoploid cell has 88 chromosomes. Both octoploids and mixoploids grew slower than tetraploids under in vitro conditions. Morphological characterizations showed that mixoploid and octoploid leaves had thicker leaf blades, thicker midrib, bigger stomata size, lower stomata density, higher SPAD value and smaller pith layer than tetraploids. This indicates that polyploidization has changed and resulted in traits that are predicted to increase photosynthetic capacity of N. cadamba. These novel polyploid plants could be valuable resources for advanced N. cadamba breeding programs to produce improved clones for planted forest development.

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Section: Discussionmentioning

confidence: 99%

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Eng

Ling³

2021

PeerJ

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“…Similar to tuberization in potatoes, CCC showed a prompting effect on in vitro PLB regeneration in Phalaenopsis orchid [ 26 , 46 ]. In vitro treatment of growth retardants, e.g., paclobutrazol, has an additional advantage in increasing in vitro to ex vitro transfer efficiency [ 47 , 48 ]. The positive regulation of growth retardants in micropropagation cannot be ignored.…”

Section: In Vitro Cultivation Factorsmentioning

confidence: 99%

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Mumivand

Akter

et al. 2022

Plants

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Ornamentals come in a variety of shapes, sizes, and colors to suit a wide range of climates, landscapes, and gardening needs. Compared to demand, a shortage of plant materials and diversity force the search for solutions for their constant acquisition and improvement to increase their commercial value, respectively. In vitro cultures are a suitable solution to meet expectations using callus culture, somatic embryogenesis, protoplast culture, and the organogenesis of protocorm-like bodies; many of these techniques are commercially practiced. Factors such as culture media, explants, carbohydrates, plant growth regulators, and light are associated with the success of in vitro propagation. Techniques, especially embryo rescue and somatic hybridization, are widely used to improve ornamentals. The development of synthetic seed allows season-independent seed production and preservation in the long term. Despite the advantages of propagation and the improvement of ornamentals, many barriers still need to be resolved. In contrast to propagation and crop developmental studies, there is also a high scope for molecular studies, especially epigenetic changes caused by plant tissue culture of ornamentals. In this review, we have accumulated and discussed an overall update on cultivation factors, propagation techniques in ornamental plant tissue culture, in vitro plant improvement techniques, and future perspectives.

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“…Among others, it is used to induce flowering, fruiting and prevent fungal infections (Wang et al, 2019). PBZ has many uses in in vitro culture for different plants, such as shoot multiplication medium MS + 5 mgL -1 in Phoenix dactylifera (Awadh et al, 2019), rooting medium 1/2 B5 + 0.1 mgL -1 in N. cadamba (Mok & Ho, 2019) and rooting medium MS + 1.5 mgL -1 in Zygopetalum crinitum (Gimenes et al, 2018).…”

Section: In Vitro Propagation and Acclimatizationmentioning

confidence: 99%

Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

Liston¹

2021

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Paclobutrazol on <i>In Vitro</i> Growth and Development of <i>Zygopetalum crinitum</i> Orchid, and on Seedling Acclimatization

Cited by 10 publications

References 13 publications

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

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