1994
DOI: 10.1093/oxfordjournals.pcp.a078569
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Paclobutrazol Protects Wheat Seedlings from Heat and Paraquat Injury. Is Detoxification of Active Oxygen Involved?

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Cited by 50 publications
(12 citation statements)
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“…The reaction mixture contained 2.4 mL of 0.1 M potassium phosphate buffer (pH 7.4), 0.3 mL 2% H 2 O 2 , 100 μL of the enzyme extract, and 1 mL of 50 mM guaiacol. Catalase (CAT) activity was measured according to the consumption of H 2 O 2 at 240 nm (Trevor et al, 1994). One unit of CAT activity was defined as the amount of enzyme catalyzing the decomposition of 1 nmol of H 2 O 2 per min.…”
Section: Antioxidant Enzymes Activities Measurementmentioning
confidence: 99%
“…The reaction mixture contained 2.4 mL of 0.1 M potassium phosphate buffer (pH 7.4), 0.3 mL 2% H 2 O 2 , 100 μL of the enzyme extract, and 1 mL of 50 mM guaiacol. Catalase (CAT) activity was measured according to the consumption of H 2 O 2 at 240 nm (Trevor et al, 1994). One unit of CAT activity was defined as the amount of enzyme catalyzing the decomposition of 1 nmol of H 2 O 2 per min.…”
Section: Antioxidant Enzymes Activities Measurementmentioning
confidence: 99%
“…However, in the case of R. sativus (Figure 4d), the increasing concentration of the tested compounds in soil caused a constant increase in the amount of carotenoids in green parts of the plant [45]. Carotenoids are necessary to protect chlorophyll against exposed biotic and abiotic factors, but the dependence of carotenoid level on the stress conditions is ambiguous [46,47,48,49]. One of the most important environmental factors affecting the level and metabolism of carotenoids is oxidative stress, which occurs when the absorbed energy exceeds the capacity of the photosynthetic plant device.…”
Section: Resultsmentioning
confidence: 99%
“…The PPO activity was determined using 0.05 M catechol as substrate, as described by Zauberman et al (1991). The POD activity was measured using the guaiacol method as described by Kraus and Fletcher (1994). All these measurements were biologically repeated for six times.…”
Section: Methodsmentioning
confidence: 99%