Padrão de distribuição de células mononucleares de medula óssea em tecido cardíaco sadio por diferentes vias de infusão

Samoto, Vívian Yochiko; Branco, Érika; Ferreira, Guilherme José Bolzanio de Campos; Cabral, Richard Haltí; Gregores, Guilheme Buzon; Sousa, A; Dohman, Hans; Silva, Suzana Alves; Takiya, Christina Maeda; Rossi, Maria Isabel D.; Borojević, Radovan; Miglino, María Angélica

doi:10.1590/s2179-83972009000200015

Cited by 3 publications

(6 citation statements)

References 25 publications

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“…An important characteristic of the studied routes was the large presence of labeled cells in other organs such as the lungs, probably due to the first-pass effect in organs related with hematopoiesis and lymphopoiesis, such as the liver and the spleen. Findings were also observed in pre-clinical studies of our and other groups 11,14 . The percentage of cells that were retained early in the IAC route …”

Section: Discussionsupporting

confidence: 86%

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Injeção intracoronariana de células tronco após infarto do miocárdio: subestudo da microcirculação

Moreira¹,

Haddad²,

Silva³

et al. 2011

Arq. Bras. Cardiol.

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Section: Discussionsupporting

confidence: 86%

“…One milliliter of labeled cells (see below) was diluted in a solution containing 100 million mononuclear cells. During the first insufflation to stop the anterograde flow, 11 ml of the final cell solution were infused through the central lumen of the venous catheter balloon for one minute 11 .…”

Section: Bmmc Delivery Techniquesmentioning

confidence: 99%

Injeção intracoronariana de células tronco após infarto do miocárdio: subestudo da microcirculação

Moreira¹,

Haddad²,

Silva³

et al. 2011

Arq. Bras. Cardiol.

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“…As described by Samoto et al (2009) in the separation of bone marrow of dogs, the lipid accumulation was not detected, a fact recorded in swine's by Branco et al (2012); however, despite the liquid consistency of the material, we could notice the formation of small cell aggregates, independent of the use of anticoagulant at the time of collection, this fact was also observed by Alves et al (2009) when they performed extraction of bone marrow from horses sternum. The authors also reported the presence of spicules in the samples, as well as gelatinous consistency of all the material, which was not observed in our study.…”

Section: Discussionsupporting

confidence: 72%

“…More evident than in dogs (Samoto et al 2009), the halo formation of bone marrow mononuclear cells from C. aethiops, after cellular separation by gradient density is quite visible, making it easy to collect, in the same way as described by Branco et al (2012) in swine, allowing the formation of pellet.…”

Section: Discussionmentioning

confidence: 85%

Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

Branco

Silva²,

Assis³

et al. 2016

Pesq. Vet. Bras.

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RESUMO.-[Protocolo para colheita e isolamento de cé-lulas mononucleares de medula óssea em Chlorocebus aethiops.] Chlorocebus aethiops é uma espécie de primata não humano frequentemente utilizados em pesquisa biomédica. Algumas pesquisas envolve esta espécie como modelo experimental para várias doenças e possível tratamento com células-tronco. A medula óssea é uma das principais fontes destas células e proporciona fácil acesso. O objetivo deste estudo foi o de padronizar o protocolo de coleta e separação de medula óssea em C. aethiops. Dez animais foram submetidos a punção de medula óssea com acesso à crista ilíaca e separação de células por gradiente de densidade. Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy. INDEX TERMS: Bone marrow, stem cell, mononuclear cells, Chlorocebus aethiops. Protocol1 Received on January 1, 2015.Accepted for publication on December 10, 2015. A medula óssea de C. aethiops tinha uma média de 97% de viabilidade. A partir dos resultados obtidos, podemos concluir que C. aethiops é um excelente modelo para obter e isolar células mononucleares da medula óssea, promovendo vários estudos no campo da terapia celular.

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“…It was observed that all samples had large amount of fat droplets, unlike what was described by Samoto et al (2009) in the separation of bone marrow of dogs. However, although the liquid consistency of the material, we could still see the formation of small cellular aggregates, regardless the use of anticoagulants for sample collection.…”

Section: Resultsmentioning

confidence: 50%

Bone marrow cells of swine: Collection and separation

Branco

Cabral

Gomès

et al. 2012

Microscopy Res & Technique

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Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.

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Padrão de distribuição de células mononucleares de medula óssea em tecido cardíaco sadio por diferentes vias de infusão

Cited by 3 publications

References 25 publications

Injeção intracoronariana de células tronco após infarto do miocárdio: subestudo da microcirculação

Injeção intracoronariana de células tronco após infarto do miocárdio: subestudo da microcirculação

Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

Bone marrow cells of swine: Collection and separation

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