Padronização de modelo de carcinogênese mamária induzido quimicamente por DMBA em camundongos

Avanzo, Gabriela Uliana

doi:10.11606/d.10.2009.tde-17042009-155223

Cited by 2 publications

(3 citation statements)

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“…for 7 weeks, (iii) PHY 4 mg/kg (p.o., once per week), and (iv) cyclophosphamide (25 mg/kg, i.p., once per week). DMBA was diluted in vegetable oil and applied at 0.2 mL weekly by gavage . Tumor formation was monitored during the 7 weeks by physical palpation and measurement using a digital caliper to determine the length ( C ) and the lagura ( L ) to calculate the tumor volume by the formula 4/3 × π × ( C /2) × ( L /2) 2.37.…”

Section: Methodsmentioning

confidence: 99%

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

et al. 2018

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Phytol (PHY) (3,7,11,15‐tetramethylhexadec‐2‐en‐1‐ol) exhibits various pharmacological properties including toxicity and cytotoxicity, and exerts antitumor activity. Owing to the urgent need of new pharmaceutical formulations for breast cancer therapy, this study aimed at the evaluation of antitumor activity of PHY in 7,12‐dimethylbenzanthracene‐cancer‐induced animal model. Comet assay was employed to evaluate the cytogenetics, DNA repair, and antigenotoxic activities of PHY in neoplastic (breast) and non‐neoplastic rodent cells (bone marrow, lymphocytes, and liver). Additionally, hematological, biochemical, histopathological, and immunohistochemical analyses were carried out in experimental animals. Thirty nonpregnant female mice (n = 5) underwent 7 weeks treatment with 6 mg/kg pro‐carcinogen, PHY (4 mg/kg), and cyclophosphamide (25 mg/kg). Induction of cancer was confirmed by histopathology and immunohistochemistry for Ki‐67. Results suggest that PHY exhibits low toxicity in comparison with other groups in hematological, biochemical, histopathological, and organ size parameters. Additionally, PHY showed modulatory effects on the pro‐carcinogen, and induced genotoxicity and apoptosis in breast cancer cells. Furthermore, it showed a DNA damage repair capacity in mouse lymphocytes. These data indicate that PHY may have the potential as an anticancer candidate in pharmaceutical consumption. © 2018 IUBMB Life, 71(1):200–212, 2019

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Section: Methodsmentioning

confidence: 99%

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

et al. 2018

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“…BERN, 1977). O menor período em camundongos foi 10 semanas (LANE et al, 1985;CURRIER et al, 2005;AVANZO, 2008).…”

Section: óRgãos Com Neoplasialesõesunclassified

“…Interessantemente, Avanzo (2008) observou o início do desenvolvimento neoplásico a partir da semana 10 nas doses menores, 1 e 3 mg, e na dose de 9 mg a partir apenas da semana 16. Embora tenha demorado mais para o início, mostrou evolução mais rápida.…”

Section: óRgãos Com Neoplasialesõesunclassified

Carcinogênese induzida por 7,12-dimetilbenzantraceno em camundongos selvagens e geneticamente modificados com deleção em um dos alelos do gene da conexina 43

Oliveira¹

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AgradecimentosThe role of the intercellular communication of gap junctions and of the proteins that form these junctions, the connexins, has been the subject of numerous studies in the field of oncology. In order to better understand the molecular mechanisms involved in the carcinogenic process and develop new weapons against cancer, these studies have shown promising, but with many questions still to be answered. Aiming to evaluate the interference connexin 43 in the carcinogenic process the carcinogen DMBA, one aromatic hydrocarbon polycyclic, was administered to genetically modified BALB;c mice heterozygous for the connexin 43 (Cx43 +/-) and wild-type (Cx43 +/+). The development of cancer occurred in 100% of animals receiving DMBA, but in different timing, types and number of tumors. In total, six types of neoplasm were observed, including breast, lymphoma, lung, gastric, skin and ovarian cancers, in that order of prevalence. Regarding breast cancer, abdominal breasts were the most affected and adenoacanthoma was the most common histological type. In the lung, stomach and skin, the most common tumor type in each was, respectively, papillary alveolar adenocarcinoma, squamous cell carcinoma and keratinizing squamous cell carcinoma. There was statistically significant difference in the incidence of ovarian tumors among groups Cx43 + / -and Cx43 + / -, indicating interference of the expression of Cx43 +/-in the carcinogenic process. Only animals of the Cx43 + / -developed this tumor type, which was represented exclusively by granulosa cell tumors. There was no statistically significant difference in the incidence of other tumors, although in absolute numbers, the incidence of almost all, except for skin cancers, was higher in Cx43 + / -. The same was repeated with respect to the development of metastases, wereas observed only in breast and gastric cancers. The use of high doses (9 mg) of DMBA appears to interfere with the carcinogenic response, most notably in the lung. Connexins act in complex and variable ways among different tumors and understanding of the relationship of connexins in cancer depends on understanding the molecular control of expression of connexins. With this work we hope to contribute to the development of studies about role of connexins in the carcinogenic process and thus help in developing ways to prevent and fight cancer.

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Padronização de modelo de carcinogênese mamária induzido quimicamente por DMBA em camundongos

Cited by 2 publications

References 10 publications

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

Carcinogênese induzida por 7,12-dimetilbenzantraceno em camundongos selvagens e geneticamente modificados com deleção em um dos alelos do gene da conexina 43

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