Paecilomyces fumosoroseus blastospore production using liquid culture in a bioreactor

Abstract

There are many advantages to using liquid cultures for the production of blastospores. These include mainly the processes of scale up which are relatively easy, as well as the control of parameters such as temperature, aeration and pH. In this work, we evaluated the production of Paecilomyces fumosoroseus blastospores using a low-cost liquid culture medium in a fermenter in comparison to a medium commonly used for this purpose, with regard to yield and viability of blastospores. The two media contained the same concentration of glucose but differed in N source (M1 containing casamino acids and M2 provided with collagen peptone and yeast extract). Starting with an inoculum of 1x10 6 blastospores/ml, M2 medium produced 2x10 10 blastospores/ml after incubation for 72 h at 520 rev/min agitation and 1 v/v/m (volume air/volume liquid.min) aeration, while only 2.4 x 10 8 /ml were produced with M1. In addition, the microorganisms in medium M1 grew more slowly during log phase and reached an earlier plateau at 36 h fermentation. The medium containing collagen peptone and yeast extract is an excellent alternative for the production of P. fumosoroseus blastospores, providing lower cost, higher yield and shorter propagation time, but formulation does need to be improved.

Effectiveness of <i>Cordyceps fumosorosea</i> Wettable Powder Formulation against <i>Metisa plana</i> (Walker) and Its Side Effects on <i>Elaeidobius kamerunicus</i> in Oil palm Plantation

“…Mass production of these propagules is easier and can be achieved within a shorter period of time by shaking the inoculated liquid culture medium. 31) The C. fumosorosea concentration produced in liquid media was approximately 1.9×10 8 CFU mL −1 . This result is still within the range produced with Jackson, Paris, and Catroux media, which was 1.4-5.5×10 8 propagules mL −1 after 3×10 6 propagules mL −1 .…”

Diaphorina citri(Hemiptera: Psyllidae) Infection and Dissemination of the Entomopathogenic FungusIsaria fumosorosea(Hypocreales: Cordycipitaceae) Under Laboratory Conditions

“…Blastospores of different entomopathogenic fungi, including Ifr have been used extensively in pest management programs on a worldwide scale (Avery 2002). Ifr blastospores easily can be mass produced in a shake-flask liquid culture medium (Jackson et al 1997(Jackson et al , 2003Lozano-Contreras et al 2007) and only require 6-8 h to germinate (Vega et al 1999). Considering that southern and central Florida experiences high humidity, it seems that the environmental conditions are conducive for the use of this fungal biopesticide as part of an IPM program in managing all stages of the psyllid population.…”

Comparative Growth and Efficacy of Trinidadian Stra Comparative Growth and Efficacy of Trinidadian Strains Trinidadian Strains of Isaria Fumosorosea Blastospores Blastospores for Controlling for Controlling for Controlling Trialeurodes Vaporariorum Trialeurodes Vaporariorum on Bean Plants Bean Plants

“…Inch et al (1986) first reported that blastospores of I. farinosa and I. fumosorosea isolates could be produced in high numbers (1.19 x 10 8 blastospores ml -1 ) in 89 hours. Following this report, production of blastospores and submerged conidia of I. fumosorosea has been accomplished by 02 various workers (Shimizu and Aizawa, 1988;de la Torre and Cardenas-Cota, 1996;Jackson et al, 1997;Lozano-Contreras et al, 2007;Asaff et al, 2009;Kim et al, 2013). Blastospores, typically larger than aerial conidia and with thin cell walls are not amenable to simple drying techniques and tend to perish more rapidly during storage than conidia (Inch et al, 1986;Bidochka et al, 1987;Lane et al, 1991;Hegedus et al, 1992).…”