Paediatric reference intervals are heterogeneous and differ considerably in the classification of healthy paediatric blood samples

Alnor, Anne; Vinholt, Pernille Just

doi:10.1007/s00431-019-03377-w

Cited by 14 publications

(17 citation statements)

References 25 publications

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“…[10][11][12][13][14][15][16] In 2015, Adeli and colleagues established robust reference intervals for 16 hematology parameters in healthy Canadians aged 3 to 79 years (n = 11999) on the Beckman Coulter HmX benchtop instrument. 10 Pediatric hematology reference intervals have also been established on larger analytical platforms, including the Siemens Advia, 11,12 Abbott Cell-Dyn, 13,16 and Beckman Coulter GenST. 14,15 However, most direct reference interval studies do not include neonates, infants, and/or young children and/or do not report findings for all CBC components (ie, erythrocytes, leukocytes, and platelets).…”

Section: Introductionmentioning

confidence: 99%

“…14,15 However, most direct reference interval studies do not include neonates, infants, and/or young children and/or do not report findings for all CBC components (ie, erythrocytes, leukocytes, and platelets). 12,[14][15][16] A few studies have established continuous pediatric hematology reference intervals on the widely used Sysmex analytical platform using only patient data extracted from tertiary laboratory information systems and complex statistical procedures. [17][18][19] These data have contributed positively to the literature, but further studies with more exclusion criteria are needed to compare robustness of each approach.…”

Section: Introductionmentioning

confidence: 99%

“…These initiatives, however, largely focus on chemistry and immunoassays and have not expanded the testing to hematology parameters until recently. A few groups have published direct reference value data for hematology parameters in healthy children and adolescents 10–16 . In 2015, Adeli and colleagues established robust reference intervals for 16 hematology parameters in healthy Canadians aged 3 to 79 years (n = 11999) on the Beckman Coulter HmX benchtop instrument 10 .…”

Section: Introductionmentioning

confidence: 99%

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Complex biological patterns of hematology parameters in childhood necessitating age‐ and sex‐specific reference intervals for evidence‐based clinical interpretation

Bohn

Higgins

Tahmasebi

et al. 2020

Int J Lab Hematology

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Introduction: Hematology laboratory parameters are among the most routinely ordered tests in support of adult and pediatric care. However, appropriate interpretation of test results has been a challenge in pediatrics since accurate and up-to-date reference intervals that reflect the dynamic physiological changes associated with growth and development have not been available. Critical gaps continue to exist in pediatric hematology reference intervals for modern laboratory platforms. To address this gap, this study establishes age-and sex-specific reference intervals for 25 hematology parameters in the CALIPER cohort of healthy children and adolescents using a common platform, the Sysmex XN-3000 analytical system. Methods: Fresh whole blood samples collected from a total of 641 healthy children and adolescents (birth to <21 years) with informed consent were analyzed for 25 hematological parameters on the Sysmex XN-3000 Hematology Analyzer. Age-and sex-specific reference standards were calculated based on Clinical and Laboratory Standards Institute guidelines. Results: Of the 25 analytes assessed, 19 required age-partitioning and seven required sex-partitioning (ie, red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, red blood cell distribution width-SD, red blood cell distribution width-CV, and monocyte percentage). Age-and sex-specific differences mostly coincided with the onset of puberty. Conclusion: This study establishes a comprehensive database of pediatric reference intervals for hematology parameters in the CALIPER cohort using the widely used Sysmex XN-3000 analytical platform. These data highlight the dynamic hematological profile observed in healthy children and adolescents and the need for reference interval stratification by age and sex.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Complex biological patterns of hematology parameters in childhood necessitating age‐ and sex‐specific reference intervals for evidence‐based clinical interpretation

Bohn

Higgins

Tahmasebi

et al. 2020

Int J Lab Hematology

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“…Frequently, the reference interval takes the central 95% of a reference population, but exceptions from this rule are observed (e.g., cTn-cardiac troponins [72] and glucose levels [73] with <5% deviation from reference intervals) [74, 75]. The reference ranges could be different among laboratories [76, 77], genders and/or ages [78], populations [79] (with variations inclusive within the same population [80, 81]), and to physiological conditions (e.g., pregnancy [82], time of sample collection, or posture). Within-subject biological variation is smaller than the between-subject variation, so reference change values could better reflect the changes in measurements for an individual as compared to reference ranges [83].…”

Section: Anatomy Of a Diagnostic Testmentioning

confidence: 99%

Medical Diagnostic Tests: A Review of Test Anatomy, Phases, and Statistical Treatment of Data

Bolboacă

2019

Computational and Mathematical Methods in Medicine

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Diagnostic tests are approaches used in clinical practice to identify with high accuracy the disease of a particular patient and thus to provide early and proper treatment. Reporting high-quality results of diagnostic tests, for both basic and advanced methods, is solely the responsibility of the authors. Despite the existence of recommendation and standards regarding the content or format of statistical aspects, the quality of what and how the statistic is reported when a diagnostic test is assessed varied from excellent to very poor. This article briefly reviews the steps in the evaluation of a diagnostic test from the anatomy, to the role in clinical practice, and to the statistical methods used to show their performances. The statistical approaches are linked with the phase, clinical question, and objective and are accompanied by examples. More details are provided for phase I and II studies while the statistical treatment of phase III and IV is just briefly presented. Several free online resources useful in the calculation of some statistics are also given.

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“…Reference intervals (RIs) play a critical role in clinical practice. Appropriate RIs for platelet parameters ensure clinical laboratories provide reliable information and enable clinicians to correctly interpret results and further determine whether transfusions are needed for neonates [1,2]. However, few normative data are available in RIs for platelet parameters in full-term neonates [3] Neonates are in a crucial period of rapid development, and platelet parameters are significantly affected by these physiological changes [4].…”

Section: Introductionmentioning

confidence: 99%

Capillary blood reference intervals for platelet parameters in healthy full-term neonates in China

et al. 2020

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Background No consensus has been reached on capillary blood reference intervals for platelet parameters in full-term neonates. We aimed to establish neonatal capillary blood reference intervals for platelet parameters and evaluate influences of sex, gestational age and postnatal age on platelet parameters. Methods This study was a prospective investigation and implemented in 594 healthy full-term neonates from 12 to 84 h of age, using SYSMEX XN-9000 haematology automatic analyser by means of capillary blood. Reference intervals for platelet parameters were defined by an interval of 2.5th − 97.5th percentiles. Results Capillary reference interval for platelet count was (152–464) × 109/L. No significance was found between sex-divided reference intervals for platelet parameters. The values of platelet count changed minimally across gestational age (37–41 weeks) and postnatal age (12–84 h). Reference intervals for other platelet parameters were affected by these factors to a different extent. Conclusions We established capillary blood reference intervals for platelet parameters in the first days after birth of full-term neonates in China.

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Paediatric reference intervals are heterogeneous and differ considerably in the classification of healthy paediatric blood samples

Cited by 14 publications

References 25 publications

Complex biological patterns of hematology parameters in childhood necessitating age‐ and sex‐specific reference intervals for evidence‐based clinical interpretation

Complex biological patterns of hematology parameters in childhood necessitating age‐ and sex‐specific reference intervals for evidence‐based clinical interpretation

Medical Diagnostic Tests: A Review of Test Anatomy, Phases, and Statistical Treatment of Data

Capillary blood reference intervals for platelet parameters in healthy full-term neonates in China

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