Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes

Abstract

An emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds’ extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from a buckwheat honey sample presented a wide spectrum of antimicrobial activity against both Gram-positive and Gram-negative pathogens. Recent investigation has confirmed that P. alvei MP1 (deposited at DDBJ/ENA/GenBank under the accession WSQB00000000) produces a proteinaceous, heat-stable compound(s) with the maximum antimicrobial production obtained after 18 h of P. alvei MP1 growth in LB medium at 37 °C with continuous shaking at 200 RPM. The highest activity was found in the 40% ammonium sulfate precipitate, with high activity also remaining in the 50% and 60% ammonium sulfate precipitates. Moderate to high antimicrobial activity that is insensitive to proteases or heat treatment, was confirmed against pathogenic bacteria that included L. monocytogenes FSL – X1-0001 (strain 10403S), S. aureus L1 – 0030 and E. coli O157: H7. Further studies, including de novo sequencing of peptides by mass spectrometry, are in progress.

Honey as an Ecological Reservoir of Antibacterial Compounds Produced by Antagonistic Microbial Interactions in Plant Nectars, Honey and Honey Bee

“…However, recent evaluation of phenotypic changes in bacterial cells treated with honey might suggest target-specific effects, for which the non-specific action of H 2 O 2 or MGO could not be accounted for [ 20 , 21 , 22 , 23 , 24 ]. The observed effects resembled the action of β-lactams [ 23 ], antimicrobial peptides [ 25 ] or inhibitors of proton motive force and chemiosmosis [ 26 ]. These data indicate that additional sources of antimicrobial compounds of honey might exist to explain the observed changes in bacterial phenotypes.…”

“…So far, three Paenibacillus strains were found in honey, P . alvei , P. polymyxa and P. larvae [ 25 , 61 , 144 , 145 ].…”

Influence of soil microbes on Escherichia coli O157:H7 survival in soil rinse and artificial soil

“…Paenibacillus spp. are known to produce colistin A and B, and other secondary metabolites that have shown antibacterial activity (Grady et al 2016;Pajor et al 2020a). Characterizing the secondary by-products of the P. alvei strain recovered in the current study would help determine potential mechanism(s) of E. coli O157:H7 reduction due to these compounds.…”

“…Characterizing the secondary by-products of the P. alvei strain recovered in the current study would help determine potential mechanism(s) of E. coli O157:H7 reduction due to these compounds. For example, Pajor et al (2020a) observed antimicrobial activity of P. alvei MP1 against E. coli O157:H7, and the heat-stable proteinaceous compounds identified in their study continue to be investigated (Pajor et al 2020b). Minimal reduction in E. coli O157:H7 was observed in filtered soil rinse subjected to P. polymyxa ATCC 21830, which has previously been documented as antagonistic through antibiotic production mechanisms (Naghmouchi et al 2012).…”

Biogenic synthesis of silver nanoparticles using Paenibacillus sp. in-vitro and their antibacterial, anticancer activity assessment against human colon tumour cell line

“…The antimicrobial activities of some Paenibacillus sp. have been reported and the results have proved that the species of genus Paenibacillus possess good antimicrobial activities against many pathogenic Gram-positive and Gram-negative bacteria, including some fungi (Aw et al, 2016;Huang et al, 2017;Pajor et al, 2020;Zhang et al, 2020). Keeping in view the in-vitro antimicrobial studies of different species of Paenibacillus, the present investigation aimed to study the antimicrobial activity and potential anticancer activity of synthesized AgNPs from the isolated bacterium Paenibacillus sp.…”