Paenibacillus residui sp. nov., isolated from urban waste compost

Abstract

Two bacterial strains, MC-246T and MC-247, were isolated from municipal urban waste compost and characterized by a polyphasic approach. Both isolates were Gram-stain-variable, endospore-forming rods that were catalase-, oxidase- and β-galactosidase-positive, and able to grow at 25–50 °C and pH 7.0–9.0, with optimum growth at 37 °C and pH 7. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0; the major respiratory quinone was menaquinone MK-7; the cell wall peptidoglycan was of type A1γ; and the DNA G+C content was 49 mol%. These characteristics, as well as data from 16S RNA gene sequence analysis, showed that these strains were affiliated with the genus Paenibacillus; the type strains of Paenibacillus ginsengarvi and Paenibacillus hodogayensis were among their closest neighbours (<94.2 % sequence similarity). Nevertheless, the hypothesis that strains MC-246T and MC-247 could represent a novel species was supported by the low 16S rRNA gene sequence similarity values shared with other members of the genus Paenibacillus and by the observation of distinct biochemical and physiological traits. Strains MC-246T and MC-247 shared 99.6 % 16S rRNA gene sequence similarity and showed almost identical MALDI-TOF mass spectra, but could be distinguished at the phenotypic and genotypic level. However, DNA–DNA hybridization between strains MC-246T and MC-247 resulted in values above 70 % indicating that both organisms represent a single species, for which the name Paenibacillus residui sp. nov. is proposed; the type strain is MC-246T (=DSM 22072T =CCUG 57263T).

Identification and Characterization of Psychrotolerant Sporeformers Associated with Fluid Milk Production and Processing

“…Interestingly, P. odorifer and P. graminis were originally isolated from plant roots as well as from pasteurized pureed vegetables (13), suggesting that that these organisms are also a potential spoilage concern in nondairy foods. In general, Paenibacillus species have been isolated from a number of environments, such as soil (37,60,67,99), rhizospheres (63,96), aquatic environments (9,10,66,86), and compost (94). Paenibacillus has only recently been recognized as a genus separate from Bacillus (8), and as many new species of Paenibacillus continue to be identified (9, 10, 12, 45, 46, 48-52, 64, 66, 86, 90, 92, 94, 97), it is becoming evident that members of this genus occupy diverse environmental niches.…”

Isolation and Characterization of Cellulose-decomposing Bacteria Inhabiting Sawdust and Coffee Residue Composts

“…Paenibacillus spp. have also been isolated from municipal urban waste compost (54), poultry litter compost (53), cow feces (55), and spent mushroom compost (57). We also found that two CRC isolates showed similarity (approx.…”

Non-contiguous finished genome sequence and description of Paenibacillus senegalensis sp. nov.

“…residui [20], the phylogenetically-closest validated Paenibacillus species (Figure 1). Although sequence similarity of the 16S operon is not uniform across taxa, this value was lower than the 98.7% 16S rRNA gene sequence threshold recommended by Stackebrandt and Ebers to delineate a new species without carrying out DNA-DNA hybridization [3].…”