Paenibacillus terrae NK3-4: A potential biocontrol agent that produces β-1,3-glucanase

Yu, Wen; Zheng, Gui Ping; Qiu, De Wen; Yan, Feng Chao; Liu, Wen Zhi; Liu, Wan Xue

doi:10.1016/j.biocontrol.2018.09.019

Cited by 27 publications

(10 citation statements)

References 72 publications

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“…SCI was remarkably effective in the control of root rot disease. However, SCII did not alleviate root rot disease in Astragalus even though it contains Bacillus velezensis and Paenibacillus terrae , species that can inhibit the growth of pathogenic fungi by producing bioactive substances [ 62 , 63 ]. Although SCI could decrease the incidence of Astragalus root rot disease, we found that the synthetic community containing four species had the same effect on root rot disease control.…”

Section: Discussionmentioning

confidence: 99%

A simplified synthetic community rescues Astragalus mongholicus from root rot disease by activating plant-induced systemic resistance

et al. 2021

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Background Plant health and growth are negatively affected by pathogen invasion; however, plants can dynamically modulate their rhizosphere microbiome and adapt to such biotic stresses. Although plant-recruited protective microbes can be assembled into synthetic communities for application in the control of plant disease, rhizosphere microbial communities commonly contain some taxa at low abundance. The roles of low-abundance microbes in synthetic communities remain unclear; it is also unclear whether all the microbes enriched by plants can enhance host adaptation to the environment. Here, we assembled a synthetic community with a disease resistance function based on differential analysis of root-associated bacterial community composition. We further simplified the synthetic community and investigated the roles of low-abundance bacteria in the control of Astragalus mongholicus root rot disease by a simple synthetic community. Results Fusarium oxysporum infection reduced bacterial Shannon diversity and significantly affected the bacterial community composition in the rhizosphere and roots of Astragalus mongholicus. Under fungal pathogen challenge, Astragalus mongholicus recruited some beneficial bacteria such as Stenotrophomonas, Achromobacter, Pseudomonas, and Flavobacterium to the rhizosphere and roots. We constructed a disease-resistant bacterial community containing 10 high- and three low-abundance bacteria enriched in diseased roots. After the joint selection of plants and pathogens, the complex synthetic community was further simplified into a four-species community composed of three high-abundance bacteria (Stenotrophomonas sp., Rhizobium sp., Ochrobactrum sp.) and one low-abundance bacterium (Advenella sp.). Notably, a simple community containing these four strains and a thirteen-species community had similar effects on the control root rot disease. Furthermore, the simple community protected plants via a synergistic effect of highly abundant bacteria inhibiting fungal pathogen growth and less abundant bacteria activating plant-induced systemic resistance. Conclusions Our findings suggest that bacteria with low abundance play an important role in synthetic communities and that only a few bacterial taxa enriched in diseased roots are associated with disease resistance. Therefore, the construction and simplification of synthetic communities found in the present study could be a strategy employed by plants to adapt to environmental stress.

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Section: Discussionmentioning

confidence: 99%

A simplified synthetic community rescues Astragalus mongholicus from root rot disease by activating plant-induced systemic resistance

et al. 2021

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“…The authors also found that partially purified protease and chitinase applied either individually or in combination showed an egg damaging ratio of 25 to 36%, which is higher than those obtained with glucanase or water (8.4 to 12.6%). Yu et al (2019) associated the antifungal activities of Paenibacillus terrae strain against Magnaporthe oryzae in rice in a greenhouse and under field conditions to β-1,3-glucanase. Lee & Kim (2015) partially purified a chitinase produced by a Pseudomonas fluorescens strain and evaluated the inhibition of nematode eggs hatching.…”

Section: Discussionmentioning

confidence: 99%

“…It has been widely reported that extracellular enzymes play important roles during microbial infection against fungi and nematodes, breaking the physical and physiological integrity of cell walls and cuticles, enabling microbial penetration and colonization (Yang et al, 2013;Wang et al, 2010;Abd-Elgawad & Askary, 2018). Recent studies successfully applied enzymes as active ingredients, rather than living cells, in the control of different nematode species (Yue et al, 2011;Castaneda-Alvarez et al, 2016;Lee & Kim, 2015;Yu et al, 2019). However, the number of studies focusing in the use of enzymes as an active ingredient is yet very low in comparison with traditional biological control approaches with living cells.…”

Section: -8mentioning

confidence: 99%

Cell-wall degrading enzymes for the control of Meloidogyne javanica

Aita¹,

Heinz²,

Alves³

et al. 2020

Biotechnology Research and Innovation

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The potential of cell-wall degrading enzymes (chitinase, β-1,3 glucanase, endo-cellulase and exocellulase) in the control of M. javanica in vitro and in vivo was investigated in this study. The enzymes were produced by solid-state fermentation using Beauveria bassiana, Trichoderma harzianum and Metarhizium anisopliae, microorganisms that are used in the biological control of agricultural pests. The bioproducts were efficient in the treatments in vitro, presenting high indexes of egg hatching inhibition (66.7-87.0%) and mortality of juveniles (40.0-90.0%). The bioproduct containing enzymes from B. bassiana presented the most promising results. In vivo treatments with all bioproducts on lettuce and tomato cultures during initial stages of plant development demonstrated excellent nematicidal effects in relation to the water-treated control. On lettuce, final egg counts and juveniles in the control treatment were 141 and 452% higher than in treatments containing bioproducts, respectively. The index of penetration of M. javanica on tomato roots was 348% higher in the control treatment than in the treatments using bioproducts. The bioproducts evaluated in this study were efficient in the control of nematodes both in treatments in vitro and in vivo. The results obtained open a new perspective for the use of bioproducts containing cell-wall degrading enzymes for the control of nematodes in agriculture.

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“…These strains along with Pseudomonas sp. strain EKM23D encode cellulases ( 28 , 29 ) and subtilisin serine protease ( 30 ). These findings implicate a diversity of genetic mechanisms underlying the biocontrol activities of seed biogels, a novel microbiome ecological niche.…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequences of Pantoea agglomerans, Paenibacillus polymyxa, and Pseudomonas sp. Strains, Seed Biogel-Associated Endophytes of Cucumis sativus L. (Cucumber) and Cucumis melo L. (Cantaloupe)

Khalaf

Raizada

2020

Microbiol Resour Announc

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Paenibacillus terrae NK3-4: A potential biocontrol agent that produces β-1,3-glucanase

Cited by 27 publications

References 72 publications

A simplified synthetic community rescues Astragalus mongholicus from root rot disease by activating plant-induced systemic resistance

A simplified synthetic community rescues Astragalus mongholicus from root rot disease by activating plant-induced systemic resistance

Cell-wall degrading enzymes for the control of Meloidogyne javanica

Draft Genome Sequences of Pantoea agglomerans, Paenibacillus polymyxa, and Pseudomonas sp. Strains, Seed Biogel-Associated Endophytes of Cucumis sativus L. (Cucumber) and Cucumis melo L. (Cantaloupe)

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