Paenibacillus vietnamensis sp. nov., isolated from the rhizosphere soil of Arachis hypogaea

Abstract

A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam. The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain were determined. Cells of strain N4T were Gram-negative, aerobic, endospore-forming and rod-shaped. Strain N4T grew at 20–37 °C (optimum, 30 °C), pH 6–10 (optimum, pH 7) and 0–5 % NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively. It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 52.9 mol%. The major isoprenoid quinone was MK-7. Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids. Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus vietnamensis sp. nov. is proposed. The type strain is N4T (=KCTC 33932T= VTCC 12236T).

Paenibacillus thermotolerans sp. nov., isolated from a hot spring in Yunnan Province, south-west China

“…Currently, the genus Paenibacillus comprises 394 species (List of Prokaryotic Names with Standing in Nomenclature; www.bacterio.net/paenibacillus.html). Fifteen species, Paenibacillus rhizolycopersici [2], Paenibacillus endoradicis , Paenibacillus radicibacter , Paenibacillus radicis [3], Paenibacillus agilis , Paenibacillus cremeus , Paenibacillus terricola [4], Paenibacillus hamazuiensis [5], Paenibacillus sabuli [6], Paenibacillus vietnamensis [7], Paenibacillus allorhizoplanae [8], Paenibacillus arenilitoris [9], Paenibacillus alkalitolerans [10], Paenibacillus mellifer [11] and Paenibacillus oleatilyticus [12] were validly published in 2022 and early in 2023. The diamino acid in the peptidoglycan is meso -diaminopimelic acid and MK-7 is the major menaquinone [13].…”