Paenibacillus xylaniclasticus sp. nov., a xylanolytic-cellulolytic bacterium isolated from sludge in an anaerobic digester

Tachaapaikoon, Chakrit; Tanasupawat, Somboon; Pason, Patthra; Sornyotha, Somphit; Waeonukul, Rattiya; Kyu, Khin Lay

doi:10.1007/s12275-012-1480-3

Cited by 9 publications

(8 citation statements)

References 38 publications

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“…For instance, the amount of glucose, xylose, and mannose in FPKC by P. curdlanolyticus DSMZ 10248 was 21.75, 20.14, and 27.91 μ mol/mL, respectively. These findings are consistent with Alshelmani et al, Pason et al, Waeonukul et al, and Tachaapaikoon et al [ 7 , 34 – 37 ], who indicated that P. curdlanolyticus was capable of producing multienzyme complexes. It is also reported that genus Paenibacillus was capable of degrading various polysaccharides and was able to produce multifunctional enzymes mainly xylanases [ 38 – 40 ].…”

Section: Resultssupporting

confidence: 92%

Biodegradation of Palm Kernel Cake by Cellulolytic and Hemicellulolytic Bacterial Cultures through Solid State Fermentation

Alshelmani

Loh

Foo

et al. 2014

The Scientific World Journal

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Four cellulolytic and hemicellulolytic bacterial cultures were purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Culture (DSMZ) and the American Type Culture Collection (ATCC). Two experiments were conducted; the objective of the first experiment was to determine the optimum time period required for solid state fermentation (SSF) of palm kernel cake (PKC), whereas the objective of the second experiment was to investigate the effect of combinations of these cellulolytic and hemicellulolytic bacteria on the nutritive quality of the PKC. In the first experiment, the SSF was lasted for 12 days with inoculum size of 10% (v/w) on different PKC to moisture ratios. In the second experiment, fifteen combinations were created among the four microbes with one untreated PKC as a control. The SSF lasted for 9 days, and the samples were autoclaved, dried, and analyzed for proximate analysis. Results showed that bacterial cultures produced high enzymes activities at the 4th day of SSF, whereas their abilities to produce enzymes tended to be decreased to reach zero at the 8th day of SSF. Findings in the second experiment showed that hemicellulose and cellulose was significantly (P < 0.05) decreased, whereas the amount of reducing sugars were significantly (P < 0.05) increased in the fermented PKC (FPKC) compared with untreated PKC.

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Section: Resultssupporting

confidence: 92%

Biodegradation of Palm Kernel Cake by Cellulolytic and Hemicellulolytic Bacterial Cultures through Solid State Fermentation

Alshelmani

Loh

Foo

et al. 2014

The Scientific World Journal

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“…We found that the complex enzyme produced by strain B-6 is critical for improving lignocellulosic biomass degradation; however, the mechanisms of lignocellulose degradation and utilization are still unclear. A similar bacterial example, Paenibacillus xylaniclasticus strain TW1 [10] isolated from sludge in an anaerobic digester, is known to have a xylan degradation system, as in strain B-6. Because of differences in several phenotypic characters, such as growth temperature and acid formation [10] , we have not previously analyzed the taxonomic relationship of strains B-6 and TW1.…”

Section: Data Descriptionmentioning

confidence: 99%

“…A similar bacterial example, Paenibacillus xylaniclasticus strain TW1 [10] isolated from sludge in an anaerobic digester, is known to have a xylan degradation system, as in strain B-6. Because of differences in several phenotypic characters, such as growth temperature and acid formation [10] , we have not previously analyzed the taxonomic relationship of strains B-6 and TW1. An understanding of the genetic relationship of the two strains and differences in their xylan degradation systems was thus needed.…”

Section: Data Descriptionmentioning

confidence: 99%

“…Although analysis of the genome data of strain B-6 demonstrated its high similarity to strain TW1, a previous investigation of enzyme component patterns of both strains clearly indicated they have different xylanase profiles [ 3 , 10 ]. In addition, BLAST searching with the B-6 draft sequence as the query failed to uncover two characteristic xylanases of strain B-6, namely, Xyn10D [12] and Xyn10E [13] , in the P. xylaniclasticus TW1 genome.…”

Section: Data Descriptionmentioning

confidence: 99%

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Draft genome sequence data of Paenbacillus curdlanolyticus B-6 possessing a unique xylanolytic-cellulolytic multienzyme system

et al. 2020

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Paenibacillus curdlanolyticus B-6 is a facultative anaerobic bacterium that efficiently produces a lignocellulolytic multienzyme complex. The whole genome of P. curdlanolyticus B-6 was sequenced on an Ion GeneStudio S5 system, which yielded 74 contigs with a total size of 4,875,097 bp, 4,473 protein-coding sequences, and a G+C content of 49.7%. The genome data have been deposited in DDBJ/ENA/GenBank under accession numbers BLWM01000001–BLWM01000074. Analyses of average nucleotide identities and phylogenetic relationships of 16S rRNA sequences of Paenibacillus species revealed that strain B-6 is most closely related to Paenibacillus xylaniclasticus TW1. P. curdlanolyticus B-6 should thus be reclassified as a strain of P. xylaniclasticus .

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“…2)3) 4) Paenibacillus xylaniclasticus strain TW1, a gram-positive facultative anaerobic bacterium, was isolated as a xylanolytic bacterium from sludge in an anaerobic digester fed with pineapple wastes in Thailand. 5) The partial genome sequencing of the TW1 strain was performed to show that this strain would possess many xylanolytic enzymes. The TW1 strain has been shown to produce many xylanolytic enzymes and extracellular multienzyme complexes when incubated with 0.5 % of birch wood xylan (BWX) and secretes cellulosebinding proteins when incubated with 0.5 % of corn hull.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a GH Family 43 β-Xylosidase Having a Novel Carbohydrate-binding Module from <i>Paenibacillus xylaniclasticus</i> Strain TW1

et al. 2022

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Paenibacillus xylaniclasticus strain TW1, a gram-positive facultative anaerobic bacterium, was isolated as a xylanolytic microorganism from the wastes of a pineapple processing factory. A gene encoding one of its xylanolytic enzymes, a β-xylosidase, was cloned and sequenced. Sequence analysis revealed that this β-xylosidase, named PxXyl43A, was composed of a glycoside hydrolase (GH) family 43 subfamily 12 catalytic module and an unknown function module (UM). The full-length PxXyl43A (PxXyl43A) was heterologously expressed in Escherichia coli and purified. Recombinant PxXyl43A exhibited hydrolysis activity against both p-nitrophenyl-β-D-xylopyranoside (pNPX) and p-nitrophenyl-α-L-arabinofuranoside at specific activities of 250 and 310 mU/mg, respectively. The optimal reaction pH and temperature for pNPX hydrolysis were 7.1 and 54 ˚C, respectively. At pH 7.0 and 54 ˚C, the Km and kcat for pNPX were 1.2 mM and 2.8 0.15 s -1 , respectively. It was also discovered that the recombinant unknown function module of PxXyl43A (PxXyl43A-UM) could bind to insoluble xylans like birchwood xylan and oat spelt xylan, whereas it did not bind to cellulosic substrates such as ball-milled cellulose, carboxymethyl cellulose or lichenan. The PxXyl43A-UMʼs binding constant value Ka for oat spelt xylan was 2.0 10 -5 M -1 . These results suggest that PxXyl43A possesses a novel carbohydrate-binding module, named as CBM91, specific for xylan-containing polysaccharides.

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Paenibacillus xylaniclasticus sp. nov., a xylanolytic-cellulolytic bacterium isolated from sludge in an anaerobic digester

Cited by 9 publications

References 38 publications

Biodegradation of Palm Kernel Cake by Cellulolytic and Hemicellulolytic Bacterial Cultures through Solid State Fermentation

Biodegradation of Palm Kernel Cake by Cellulolytic and Hemicellulolytic Bacterial Cultures through Solid State Fermentation

Draft genome sequence data of Paenbacillus curdlanolyticus B-6 possessing a unique xylanolytic-cellulolytic multienzyme system

Characterization of a GH Family 43 β-Xylosidase Having a Novel Carbohydrate-binding Module from <i>Paenibacillus xylaniclasticus</i> Strain TW1

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