Paeoniflorin ameliorates rheumatoid arthritis in rat models through oxidative stress, inflammation and cyclooxygenase 2

Abstract

Paeoniflorin has anti-inflammatory, anti-allergy, immune regulatory and pain-relieving effects, amongst other roles. However, the mechanisms underlying the protective effects of paeoniflorin on rheumatoid arthritis (RA) remain under investigation; the objective of the current study was to evaluate these protective effects in the context of an RA model. Rats were randomly divided into 5 groups, as follows: The control group, the RA rat model group, and the paeoniflorin groups, in which paeoniflorin was administered at concentrations of 5, 10 and 20 mg/kg for 3 weeks. The pain thresholds and arthritic symptoms of the RA rats were measured. Oxidative stress and inflammatory cytokines were also analyzed and western blot analysis was used to evaluate cyclooxygenase-2 (COX-2) protein expression levels. Paeoniflorin significantly increased the pain threshold and decreased the arthritic symptoms in the RA rat model. Notably, paeoniflorin reduced the malondialdehyde concentration and increased the activity of superoxide dismutase, catalase and glutathione peroxidase. Furthermore, paeoniflorin attenuated the activity of nuclear factor-κB p65 unit, tumor necrosis factor-α, interleukin (IL)-1β and IL-6, and reduced the COX-2 protein expression level. The present study indicates that paeoniflorin ameliorates disease in rat models of RA through oxidative stress, inflammation and alterations to COX-2 expression.

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

“…In this research, we discovered that the wogonin therapy group had considerably reduced lipid peroxides, greater GSH, and enhanced antioxidant enzyme activity such as SOD and CAT that could decrease or stop ROS from forming. Therefore, our findings stated that the suppression of the pathological state of arthritis by antioxidant could be one mechanism of Wogonin in CFA rats …”

Mitigation of TGF-β/Smad signaling pathway-associated liver fibrosis by paeoniflorin

“…In the current study, TGF-β1 was downregulated in a dose-dependent way after PF administration. The findings in this study could be attributed to the inhibition of both the macrophages and the autocrine of HSCs, as well as the anti-inflammatory effect of PF, which have been widely reported [3,15].…”

“…Paeoniflorin (PF), isolated from Corte moutan, is a major bioactive compound with antiinflammatory and immune-regulatory effects [3,4]. The anti-fibrotic effect of PF has been studied on both pulmonary [5,6] and renal interstitial fibrosis [7].…”

Preventive Effects of the Intestine Function Recovery Decoction, a Traditional Chinese Medicine, on Postoperative Intra‐Abdominal Adhesion Formation in a Rat Model

“…In the IFRD, some constituents including Codonopsis Radix (Dangshen) [28], Atractylodes Rhizoma (Baizhu) [29], and Paeoniae Rubra Radix (Chishao) [30] were reported to have anti-inflammatory and antioxidative effects. Jia and He [31] indicated that paeoniflorin, a chemical constituent of Paeoniae Rubra Radix, ameliorated disease in rat models of rheumatoid arthritis by suppressing oxidative stress and inflammation and reducing COX-2 protein expression. Wei et al [32] and other researchers [20, 33] have provided evidence that hypoxia-induced COX-2 expression in peritoneal fibroblasts is involved in the formation of intra-abdominal adhesions.…”