Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3

Abstract

Bladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects on BCa. The results showed that Pae inhibited proliferation of human BCa cell lines in a concentration- and time-dependent manner. Pae and cisplatin (Cis) synergistically inhibited the growth of tumours in RT4-bearing mice. Pae treatment neutralized the body loss induced by Cis. Moreover, Pae induced apoptosis in RT4 cells and increased the activities of caspase3, caspase8 and caspase9. Western blotting and immunohistochemical analysis revealed that the phosphorylated signal transducer and activator of transcription-3 (p-STAT3) level were decreased in Pae-treated RT4 cells and Pae-treated tumour-bearing mice. Furthermore, STAT3 transcriptional target B-cell lymphoma-2 was decreased in Pae-treated RT4 cells. Interestingly, Pae prevented translocation of STAT3 to the nucleus in RT4 cells. Collectively, Pae inhibits the growth of BCa, at least in part, via a STAT3 pathway.

Paeoniflorin: a monoterpene glycoside from plants of Paeoniaceae family with diverse anticancer activities

“…By inhibiting pathway STAT3, the growth of bladder cancer cells is inhibited . In 2018, Yang et al . carried out both in‐vivo and in‐vitro studies and found that Paeoniflorin inhibits the growth of bladder cancer cells by inhibiting pathway STAT3.…”

“…By inhibiting pathway STAT3, the growth of bladder cancer cells is inhibited. [59] In 2018, Yang et al [73] carried out both in-vivo and in-vitro studies and found that Paeoniflorin inhibits the growth of bladder cancer cells by inhibiting pathway STAT3. In addition, another study on red peony root extract (Paeoniflorin), based on in-vivo and in-vitro excitements of Paeoniflorin on mouse bladder cancer cells, shows that the extract maintains the cells in G 1 phase and has no obvious cytotoxicity to other cells, [74] but the specific mechanism is not very clear.…”

“…Inhibit S-phase kinase-associated protein (Skp) 2, down-regulate P21 and expression of Phosphorylated (p-) AKT, thus prevent tumour progression [63] Have inhibitory effect on human BCa cell through inhibiting pathway STAT3 [73] 0, 0.5, 1, 2, 3, 3.5 mg/ml; 2.5 mg/kg per qod [75] 80, 160, 240, 320, 400, 480, 560, 640 mg/ml…”

Paeoniflorin inhibits MRGPRX2‐mediated pseudo‐allergic reaction via calcium signaling pathway

“…Paeoniflorin (PF), a monoterpene glycoside isolated from the aqueous extract of the Chinese herb Radix Paeoniae alba , is a biologically active compound (Liu, Han, Zhou, & Li, ). Currently, studies on PF mainly focus on its antitumor and neuroprotective effects (Cong et al, ; Yang et al, ; Yu et al, ), having been shown that it can protect neurons in an MPTP mouse model of Parkinson's disease (Zheng et al, ). An antiinflammatory effect of PF has been reported, being involved in preventing endoplasmic reticulum stress‐associated inflammation in lipopolysaccharide‐stimulated human umbilical vein endothelial cells (Li, Jiao, & Xie, ).…”

The Anticancer Effects of Paeoniflorin and Its Underlying Mechanisms

“…38 A RT4 cell xenograft nude mice model was established to determine if PF could inhibit bladder tumor growth in vivo, the results showed that intraperitoneal administration of PF (100 mg kg −1 day −1 ) reduced the weight and volumes of the tumor tissue. 31 Additionally, by oral gavage feeding of PF at 1 g kg −1 day −1 , PF reduced the tumor volume by 30.8% and had a stronger growth inhibitory effect and lower blood toxicity than that of docetaxel. 57 In summary, these studies indicated that PF has a variety of antitumor activities.…”