Paeoniflorin on Rat Myocardial Ischemia Reperfusion Injury of Protection and Mechanism Research

Abstract

Objective: To study the myocardial benefit effect and mechanism of paeoniflorin on myocardial ischemia reperfusion injury (MIRI) in rats. Methods: Hundred SD rats were randomly divided into 5 groups: sham group, model group, Paeoniflorin (15 mg/kg) group, Paeoniflorin (30 mg/kg) group, and Paeoniflorin (60 mg/kg) group. Myocardial ischemia reperfusion model was established in each group except the sham group. The myocardial infarction and morphological changes were measured by the TTC staining and HE staining respectively. Myocardial caspase-3 was detected by immunohistochemistry. In addition, the protein levels of Bcl-2 and Bax and the expression ratio of p-erk, p-jnk, and p-p38 were detected by Western blot. Myocardial superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were measured by the assay kit. Results: Paeoniflorin (30 mg/kg) and Paeoniflorin (60 mg/kg) can obviously alleviate myocardial infarction caused by MIRI (p < 0.05). HE staining showed that the myocardial morphology in the treatment group was obviously better than that in the model group. WB and immunohistochemistry showed that Paeoniflorin (30 mg/kg) andPaeoniflorin (60 mg/kg) can significantly increase the reduced protein level of bcl-2 (p < 0.05) and reduce the increased protein level of caspase3, bax p-erk, p-jnk, and p-p38 caused by MIRI (p < 0.05). The activity of SOD was increased and the level of MDA was decreased after Paeoniflorin treatment. Conclusion: Paeoniflorin preconditioning has a protective effect on MIRI in rats. Its mechanism is related to reducing oxidative stress and apoptosis by inhibiting the expression of apoptosis-related signaling pathway.

Asiatic Acid Alleviates Myocardial Ischemia-Reperfusion Injury by Inhibiting the ROS-Mediated Mitochondria-Dependent Apoptosis Pathway

“…In the current study, we first examined whether administration of AA affects MIRI-induced activation of the MAPK signalling pathway. As shown in Figures 7(a) and 7(b) , consistent with the results of other studies [ 41 , 42 ], the proteins derived from I-R-treated hearts or NRVMs showed elevated levels of phosphorylated p38-MAPK and JNK-MAPK. In contrast, pretreatment with AA effectively prevented MIRI-induced p38-MAPK and JNK-MAPK phosphorylation.…”

Lycium barbarum polysaccharides inhibit ischemia/reperfusion-induced myocardial injury via the Nrf2 antioxidant pathway

“…The I/R injury model was constructed as previously described [ 24 ]. Briefly, rats were anesthetized using 1.5–2 % isoflurane.…”

Oxypaeoniflorin improves myocardial ischemia/reperfusion injury by activating the Sirt1/Foxo1 signaling pathway

“…Based on the abundant evidence showing the protective effect of paeoniflorin (a structural analog of OPA) against MI/R injury (Chen et al, 2018;Han et al, 2016a;Wu et al, 2019), and the reported inhibitory effects of OPA on oxidative damage and inflammation in mesangial cells and LPS-stimulated RAW264.7 cells (Yoo et al, 2018a), we speculate that OPA may protect against the MI/R injury. Our experiments successfully verified the above hypothesis and elucidated the mechanism of action by which OPA protects against MI/R injury, namely, by regulating the Sirt1/Foxo1-mediated apoptosis signals.…”