Pairing and aggregation of <i>Amblosoma suwaense</i> (Trematoda: Brachylaimidae) metacercariae <i>in vitro</i> and partial characterization of lipids involved in chemo-attraction

Fried, Bernard; Robinson, G. A.

doi:10.1017/s003118200005695x

Cited by 17 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pairing of Echinostoma revolutum adults in vitro was observed by Fried, Tancer & Fleming (1980) and the chemoattractant again identified as a free sterol. Similar pairing was noted by adults of Amblosoma suwaense but the chemoattractant was found to be a sterol ester rather than a free sterol (Fried & Robinson, 1981). Imperia, Fried & Eveland (1980) observed significant attraction of female S. mansoni to 1-male sources in vitro but not to a 3-male source.…”

Section: Mating Behaviour Of Schistosomessupporting

confidence: 67%

Patterns of sexual reproduction among parasitic platyhelminths

Nollen

1983

Parasitology

View full text Add to dashboard Cite

Previous studies on the spermatogenesis and oogenesis of digeneans, monogeneans and cestodes are reviewed, including those in which isotopic labelling techniques have been utilized in order to determine the temporal duration of spermatogenic development stages. Similar labelling experiments have also provided information on the development and movement of oocytes in the female reproductive tract as well as indicating patterns of cell division and development in vitelline glands. The literature relating to the inseminative behaviour of parasitic platyhelminths is considered from a number of viewpoints including the patterns of cross- and self-insemination that occur in hermaphroditic digeneans, the mating behaviour of dioecious schistosomes, spermatophore production and insemination among monogeneans and the inseminative behaviour of polyzoic cestodes. The implications of selfing among parasitic platyhelminths are considered with respect to possible genetically deleterious consequences. In addition, consideration is given to the mechanisms underlying the fact that some species require cross-insemination or at least the presence of another worm in order to undergo normal growth and sexual development. Finally, experimental studies are reviewed which shed light on the strength of the barriers against hybridization that exist between well-defined species of parasitic platyhelminths.

show abstract

Section: Mating Behaviour Of Schistosomessupporting

confidence: 67%

Patterns of sexual reproduction among parasitic platyhelminths

Nollen

1983

Parasitology

View full text Add to dashboard Cite

show abstract

“…Our E-S analysis of H. medioplexus is in agreement with previous studies (Fried &Gioscia, 1976;Fried & Shapiro, 1975;Fried etal. 1980;Fried & Robinson, 1981), which indicated that sterol is a major lipid fraction released by flukes into a non-nutrient medium. The role of sterols in interspecific trematode pairing needs further study.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have demonstrated intraspecific pairing in vitro of Leucochloridiomorpha constantiae (Fried & Roberts, 1972), Echinostoma revolutum (Fried, Tancer & Fleming, 1980) and of Amblosoma suwaense (Fried & Robinson, 1981). Interspecific pairing in vitro has been reported by Fried & Jacobs (1980) matching E. revolutum against either Zygocotyle lunata or L. constantiae.…”

Section: Introductionmentioning

confidence: 89%

“…Several thin-layer chromatographic (t.l.c.) studies have indicated that sterols released by trematodes are involved in chemical attraction (L. constantiae by Fried & Gioscia (1976); E. revolutum by and A. suwaense by Fried & Robinson (1981)). Cain & French (1975) showed that cholesterol is the major lipid of//, medioplexus, accounting for almost 40% of total worm lipid.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Intraspecific and interspecific pairing of Haematoloechus medioplexus (Trematoda) and Echinostoma revolutum (Trematoda) adults in vitro, and observations on H. medioplexus lipophilic excretory–secretory products

Fried

Leiby

1982

Parasitology

Self Cite

View full text Add to dashboard Cite

SUMMARYIntraspecific pairing of Haematoloechus medioplexus and Echinostoma revolutum adults and interspecific pairing of H. medioplexus and E. revoltum adults were studied in vitro at 21 ± 1 °C and 30 ± 1 °C in Petri-dish cultures containing an agar substrate and a Locke's 1:1 solution overlay. Salinity and temperature tolerance tests showed that both species survived for 24 h in a Locke's 1:1 solution at 21 ± 1 °C and 30 ±1 °C. For behaviour studies, worms were placed 2 cm apart and then observed at various intervals from 5 min to 4 h. H. medioplexus and E. revolutum showed significant intraspecific pairing at 30±1 °C, whereas only H. medioplexus paired significantly at 21±1°C. Significant interspecific pairing of H. medioplexus and E. revolutum occurred at 21 ±1 °C and 30 ±1 °C. Thin-layer chromatographic analysis showed that H. medioplexus adults released mainly free sterol, along with traces of triacylglycerols and free fatty acids into a non-nutrient medium.

show abstract

Further studies on in vitro pairing ofEchinostoma revolutum (Trematoda) adults

Fried

Pallone

1984

Z. Parasitenkd.

View full text Add to dashboard Cite

Various factors that influence in vitro pairing of Echinostoma revolutum adults were studied. More worms paired in Locke's, Ringer's, and 0.85% NaCl solutions than in the defined medium NCTC 135. Also, more pairing occurred at 39 or 43 degrees C than at 35 degrees C. Echinostomes placed 1, 3, or 5 cm apart in 5.5 cm diameter Petri dishes as well as those placed 2, 4, or 6 cm apart in 8.5 cm diameter Petri dishes paired; however, worms placed 8 cm apart in the latter dishes did not. Individuals maintained in Locke's solution at 20 or 39 degrees C for 1 h after having been removed from the chicks paired less frequently than those used for experiments immediately after their removal from chicks. Worms showed more pairing in fresh Locke's solution than in this solution preconditioned by excretory-secretory products of E. revolutum. Scanning electron microscopy revealed no differences between the tegument of fresh worms and of those maintained under experimental conditions for 2 h at 39 degrees C.

show abstract

Pairing and aggregation of Amblosoma suwaense (Trematoda: Brachylaimidae) metacercariae in vitro and partial characterization of lipids involved in chemo-attraction

Cited by 17 publications

References 15 publications

Patterns of sexual reproduction among parasitic platyhelminths

Patterns of sexual reproduction among parasitic platyhelminths

Intraspecific and interspecific pairing of Haematoloechus medioplexus (Trematoda) and Echinostoma revolutum (Trematoda) adults in vitro, and observations on H. medioplexus lipophilic excretory–secretory products

Further studies on in vitro pairing ofEchinostoma revolutum (Trematoda) adults

Contact Info

Product

Resources

About