Pak3 regulates apical-basal polarity in migrating border cells during <i>Drosophila</i> oogenesis

Felix, Martina; Chayengia, Mrinal; Ghosh, Ritabrata; Sharma, Aditi; Prasad, Mohit

doi:10.1242/jcs.182980

Cited by 3 publications

(4 citation statements)

References 1 publication

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“…Genes encoding proteins that regulate the actin cytoskeleton were also newly accessible YAP5SA targets (Morikawa et al, 2015). Pak3 encodes a protein that regulates actin cytoskeleton by modulating small GTPase activity (Felix et al, 2015;Hiramoto-Yamaki et al, 2010), whereas Cobl and Fmn1 encode actin nucleators (Hou et al, 2015;Li et al, 2015b) (Figures 5F and S5; Table S1).…”

Section: Adherens Junction Assembly and Cytoskeletal Genesmentioning

confidence: 99%

YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo

Monroe

Hill

Morikawa³

et al. 2019

Developmental Cell

201

177

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Section: Adherens Junction Assembly and Cytoskeletal Genesmentioning

confidence: 99%

YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo

Monroe

Hill

Morikawa³

et al. 2019

Developmental Cell

201

177

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“…While a few SJ proteins have previously been reported to be expressed in the Drosophila ovary ( Wei et al 2004 ; Schneider et al 2006 ; Hall et al 2014 ; Maimon et al 2014 ; Felix et al 2015 ; Ben-Zvi and Volk 2019 ), a thorough analysis of their tissue distribution and subcellular localization throughout oogenesis is lacking. We therefore examined the spatial and temporal expression of four SJ proteins: Mcr, Cont, Nrx-IV, and Cora ( Fehon et al 1994 ; Baumgartner et al 1996 ; Faivre-Sarrailh et al 2004 ; Bätz et al 2014 ; Hall et al 2014 ).…”

Section: Resultsmentioning

confidence: 99%

“…The BC cluster, along with the migratory centripetal cells, collaborate to form the micropyle, a hole through which the sperm enters the egg ( Montell 2003 ; Horne-Badovinac 2020 ). Previous studies showed that Cora and Nrg are expressed in the PCs during their migration ( Wei et al 2004 ; Felix et al 2015 ). To determine if other SJ proteins are also expressed during BC migration, we stained stages 9 and 10 wild-type egg chambers with antibodies against Mcr, Cont, Nrx-IV, and Cora.…”

Section: Resultsmentioning

confidence: 99%

Septate junction proteins are required for egg elongation and border cell migration during oogenesis in Drosophila

Alhadyian

Shoaib

Ward

2021

G3 Genes|Genomes|Genetics

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Protein components of the invertebrate occluding junction—known as the septate junction (SJ) - are required for morphogenetic developmental events during embryogenesis in Drosophila melanogaster. In order to determine whether SJ proteins are similarly required for morphogenesis during other developmental stages, we investigated the localization and requirement of four representative SJ proteins during oogenesis: Contactin, Macroglobulin complement-related, Neurexin IV, and Coracle. A number of morphogenetic processes occur during oogenesis, including egg elongation, formation of dorsal appendages, and border cell migration. We found that all four SJ proteins are expressed in egg chambers throughout oogenesis, with the highest and most sustained levels in the follicular epithelium (FE). In the FE, SJ proteins localize along the lateral membrane during early and mid-oogenesis, but become enriched in an apical-lateral domain (the presumptive SJ) by stage 10B. SJ protein relocalization requires the expression of other SJ proteins, as well as Rab5 and Rab11 in a manner similar to SJ biogenesis in the embryo. Knocking down the expression of these SJ proteins in follicle cells throughout oogenesis results in egg elongation defects and abnormal dorsal appendages. Similarly, reducing the expression of SJ genes in the border cell cluster results in border cell migration defects. Together, these results demonstrate an essential requirement for SJ genes in morphogenesis during oogenesis, and suggests that SJ proteins may have conserved functions in epithelial morphogenesis across developmental stages. Article Summary: Septate junction (SJ) proteins are essential for forming an occluding junction in epithelial tissues in Drosophila melanogaster, and also for morphogenetic events that occur prior to the formation of the junction during embryogenesis. Here we show that SJ proteins are expressed in the follicular epithelium of egg chambers during oogenesis and are required for morphogenetic events including egg elongation, dorsal appendages formation, and border cell migration. Additionally, the formation of SJs during oogenesis is similar to that in embryonic epithelia.

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“…The most likely explanation for this dramatic beneficial effect is that basolateral Cdep feeds back into the cell polarity circuit, either by participating as a scaffolding protein for Dlg or Lgl, or through Rac activation. For example, one study has implicated Rac and the RacGTP-binding protein Pak in apicobasal polarity in border cells (Felix et al, 2015), and there is ample evidence for interdependence between the core polarity modules and Rho family GTPases as mentioned above (Mack and Georgiou, 2014). Given the dozens of Scrib module binding proteins and the complexity and redundancies amongst Rho family GEFs, it is truly remarkable that membrane tethering of Cdep alone is sufficient to substantially rescue epithelial polarity.…”

Section: Discussionmentioning

confidence: 99%

A Scribble/Cdep/Rac pathway regulates follower cell crawling and cluster cohesion during collective border cell migration

Campanale¹,

Mondo²,

Montell³

2022

Preprint

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Apicobasal polarity is a defining characteristic of epithelial cells and its disruption is a cancer hallmark. Distinct apical and basolateral protein modules antagonize each other to establish separate membrane domains. These modules interact with dozens of potential effector proteins. Here we describe polarity protein localization and function within a migrating epithelial cell cluster and identify a functionally significant effector protein. In Drosophila egg chambers, border cells delaminate from the follicular epithelium and migrate collectively. We report that the basolateral protein Scribble is required for border cell cluster cohesion and migration. The basolateral module localizes the Rac guanine nucleotide exchange factor Cdep to membranes, and Cdep knockdown phenocopies Scribble cluster cohesion defects. Remarkably, membrane targeting of Cdep is sufficient to partially suppress multiple Scribble phenotypes. We describe specialized basolateral protrusions that promote cluster cohesion. Scribble restricts these protrusions from encroaching onto the apical domain. Thus, a major function of the basolateral module is to localize Cdep, promoting specialized protrusions, cluster cohesion, and collective migration.

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Pak3 regulates apical-basal polarity in migrating border cells during Drosophila oogenesis

Cited by 3 publications

References 1 publication

YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo

YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo

Septate junction proteins are required for egg elongation and border cell migration during oogenesis in Drosophila

A Scribble/Cdep/Rac pathway regulates follower cell crawling and cluster cohesion during collective border cell migration

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