Pakistolides A and B, Novel Enzyme Inhibitory and Antioxidant Dimeric 4‐(Glucosyloxy)Benzoates from Berchemia pakistanica

Abstract

Pakistolides A and B, novel dimeric b-(glucosyloxy)benzoates were isolated from Berchemia pakistanica and assigned structures 1 and 2 on the basis of extensive NMR studies. In addition, the known compounds 7,5'-dimethoxy-3,5,2'-trihydroxyflavone ( 3,5-dihydroxy-2-(2-hydroxy-5-methoxyphenyl)-7-methoxy-4H-1-benzopyran-4-one), 4',5-dihydroxy-3,6,7-trimethoxyflavone ( 5-hydroxy-2-(4-hydroxyphenyl)-3,6,7-trimethoxy-4H-1-benzopyran-4-one), 5,6-dihydroxy-4,7-dimethoxy-2-methylanthracene-9,10-dione, and 1,3,4-trihydroxy-6,7,8-trimethoxy-2-methylanthracene-9,10-dione were reported for the first time from the genus Berchemia. Both 1 and 2 showed significant a-glucosidase and lipoxygenase inhibitory activities, while 2 also showed antioxidant potential.

Isolation of Onosmins A and B, Lipoxygenase Inhibitors from Onosma hispida

“…The assay conditions and protocol were the same as described in our previous article. 23) All kinetic study was performed in 96-well microtitre plates using SpectraMax 384plus (Molecular Devices, CA, U.S.A.). The IC 50 values were then calculated using the EZ-Fit Enzyme kinetics program (Perrella Scientific Inc., Amherst, MA, U.S.A.).…”

Astonishing diversity of natural surfactants: 2. Polyether glycosidic ionophores and macrocyclic glycosides

“…10 species scattered throughout the tropics of Asia, Africa, and America (133). The roots and stems of Berchemia species are traditionally used for the treatment of gallstones, liver diseases, neuralgia, and stomach cramps in Japan, and also are used in traditional Chinese medicine as an antipyretic, a diuretic, and for the treatment of rheumatism and lumbago (134).…”

Isolation and Lipoxygenase-Inhibition Studies of Phenolic Constituents fromEhretia obtusifolia

“…The assay conditions and protocol were those described in a previous article [24]. All kinetics studies were performed in 96-well micro-titre plates using SpectraMax 384plus (Molecular Devices, USA).…”