Palatability of transgenic birch and aspen to roe deer and mountain hare

Vihervuori, Liisa; Lyytikäinen‐Saarenmaa, Päivi; Tuomikoski, Eemeli; Luoma, Mikael; Niemelä, Pekka; Pappinen, Ari; Pasonen, Hanna-Leena

doi:10.1080/09583157.2012.716393

Cited by 3 publications

(2 citation statements)

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“…Expression of the BvSP2 (= B. vulgaris SP2 ) gene was very high in infected leaves of sugar beet plants, but BvSP2 protein accumulation was limited to the area immediately surrounding the sites of infection ( Nielsen et al, 1994 ). Sugar beet chitinase IV gene BvSP2 when expressed in transgenic birch ( Betula pendula ) inhibited growth of the insect larvae of Orgyia antiqua L., Lymantriidae ( Vihervuori et al, 2013 ) but had no adverse effects on mammalian herbivores like roe deer ( Capreolus capreolus L.) ( Vihervuori et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Two sugar beet chitinase genes,BvSP2andBvSE2, analysed with SNP Amplifluor-like markers, are highly expressed after Fusarium root rot inoculations and field susceptibility trial

Yerzhebayeva

Abekova

Konysbekov

et al. 2018

PeerJ

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BackgroundThe pathogens from Fusarium species can cause Fusarium root rot (RR) and other diseases in plant species including sugar beet (Beta vulgaris L.), and they have a strong negative impact on sugar beet yield and quality.MethodsA total of 22 sugar beet breeding lines were evaluated for the symptoms of RR after inoculation with Fusarium oxysporum Sch., isolate No. 5, and growth in a field trial. Two candidate genes for RR resistance, BvSP2 and BvSE2, encoding chitinases Class IV and III, respectively, were previously identified in sugar beet, and used for genotyping using modern Amplifluor-like single nucleotide polymorphism (SNP) genotyping approach. The qPCR expression analysis was used to verify responses of the candidate genes for RR infections.ResultsA strong association of two SNP markers for BvSP2 and BvSE2 with resistance to RR in sugar beet was found in our study. Very high BvSP2 expression (100-fold compared to Controls) was observed in three RR resistant accessions (2182, 2236 and KWS2320) 14 days after inoculation which returned to the control level on Day 18. RR sensitive breeding line 2210 showed a delay in mRNA level, reaching maximal expression of BvSP2 18 days after inoculation. The gene BvSE2, showed a strong expression level in leaf samples from the infected field trial only in the breeding line 2236, which showed symptoms of RR, and this may be a response to other strains of F. oxysporum.

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Section: Introductionmentioning

confidence: 99%

Two sugar beet chitinase genes,BvSP2andBvSE2, analysed with SNP Amplifluor-like markers, are highly expressed after Fusarium root rot inoculations and field susceptibility trial

Yerzhebayeva

Abekova

Konysbekov

et al. 2018

PeerJ

View full text Add to dashboard Cite

show abstract

“…Sugar beet chitinase IV gene BvSP2 when expressed in transgenic birch (Betula pendula) inhibited growth of the insect larvae of Orgyia antiqua L., Lymantriidae (Vihervuori et al 2013) but had no adverse effects on mammalian herbivores like roe deer (Capreolus capreolus L.) (Vihervuori et al 2012 Manuscript to be reviewed markers (He et al 2014). Amplifluor-like SNP markers have a simple design, employing a mixture of two universal probes carrying fluorescent labels and a quencher, and non-labelled gene-specific primers (Giancola et al 2006;Shavrukov et al 2016;Jatayev et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Two sugar beet chitinase genes, BvSP2 and BvSE2, analysed with SNP Amplifluor-like markers, are highly expressed after Fusarium root rot inoculations and field susceptibility trial (v0.1)"

2018

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Background. The pathogens from Fusarium species can cause Fusarium root rot (RR) and other diseases in plant species including sugar beet (Beta vulgaris L.), and they have a strong negative impact on sugar beet yield and quality. Methods. Twenty-two sugar beet breeding lines were evaluated for the symptoms of RR after inoculation with Fusarium oxysporum Sch., isolate No. 5, and growth in a field trial. Two candidate genes for RR resistance, BvSP2 and BvSE2, encoding chitinases Class IV and III, respectively, were previously identified in sugar beet, and used for genotyping using modern Amplifluor-like SNP genotyping approach. The qPCR expression analysis was used to verify responses of the candidate genes for root rot infections. Results. A strong association of two SNP markers for BvSP2 and BvSE2 with resistance to RR in sugar beet was found in our study. Very high BvSP2 expression (hundred-fold compared to Controls) was observed in three RR resistant accessions (2182, 2236 and KWS2320) 14 days after inoculation which returned to the control level on Day 18. RR sensitive breeding line 2210 showed a delay in mRNA level, reaching maximal expression of BvSP2 18 days after inoculation. The gene BvSE2, showed a strong expression level in leaf samples from the infected field trial only in the breeding line 2236, which showed symptoms of RR, and this may be a response to other strains of F. oxysporum.

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Lessons from 25 Years of GM Tree Field Trials in Europe and Prospects for the Future

Pilate¹,

Allona²,

Boerjan³

et al. 2016

Biosafety of Forest Transgenic Trees

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Palatability of transgenic birch and aspen to roe deer and mountain hare

Cited by 3 publications

References 46 publications

Two sugar beet chitinase genes,BvSP2andBvSE2, analysed with SNP Amplifluor-like markers, are highly expressed after Fusarium root rot inoculations and field susceptibility trial

Two sugar beet chitinase genes,BvSP2andBvSE2, analysed with SNP Amplifluor-like markers, are highly expressed after Fusarium root rot inoculations and field susceptibility trial

Peer Review #2 of "Two sugar beet chitinase genes, BvSP2 and BvSE2, analysed with SNP Amplifluor-like markers, are highly expressed after Fusarium root rot inoculations and field susceptibility trial (v0.1)"

Lessons from 25 Years of GM Tree Field Trials in Europe and Prospects for the Future

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