Paleic acid, a fatty acid from Paenibacillus sp.: taxonomy, fermentation, isolation, structure determination, and anti-Mannheimia and -Pasteurella activity

Kurata, Ikuko; Umekita, Maya; Sawa, Tsutomu; Hattori, Shigehiko; Hayashi, Chikara; Kinoshita, Naoko; Homma, Yoshiko; Igarashi, Masayuki; Hamada, Masa; Watanabe, Takumi; Sawa, Ryûichi; Naganawa, Hiroshi; Takahashi, Yoshikazu; Akamatsu, Yuzuru

doi:10.1038/ja.2010.90

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Therefore, other minor compounds, which could possibly be highly ionized, appeared as major compounds besides the true major compounds. Different FAs and their derivatives, including monohydroxy FAs [41], polyhydroxy FAs [42], and keto acids [43], have been isolated from Streptomyces.…”

Section: Discussionmentioning

confidence: 99%

Isolation and optimized production of putative antimicrobial compounds from Egyptian soil isolate Streptomyces sp. MS. 10

Sebak

Saafan

AbdelGhani

et al. 2021

Beni-Suef Univ J Basic Appl Sci

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Background The rapid spread of antibiotic resistance has increased research interest in the discovery of natural products, mainly from actinomycetes, which have been the primary source of antimicrobial compounds. This study aimed to isolate, characterize, and optimize the production of some of the bioactive compounds from bioactive soil actinomycetes. Results One promising soil actinomycete, which was molecularly identified as Streptomyces sp. and designated as Streptomyces sp. MS. 10, showed broad-spectrum antimicrobial activity, including activity against methicillin-resistant Staphylococcus aureus. Thus, it was selected for isolation of its major bioactive compounds. Polymerase chain reaction amplification of the genes responsible for antibiotic biosynthesis showed the presence of genes encoding type I and type II polyketide synthase. Liquid chromatography-mass spectrometry analysis found that the major antimicrobial compounds produced by Streptomyces sp. MS. 10 were weakly ionized bioactive secondary metabolites. A large-scale fermentation experiment of Streptomyces sp. MS. 10 using pre-optimized culture conditions followed by bioassay-guided chromatographic separation of its secondary metabolites resulted in the isolation of putative bioactive compounds that were identified as fatty acids using proton nuclear magnetic resonance spectroscopy. Conclusions Egyptian soil is still a good source for exploring bioactive actinomycetes. Additionally, this study highlighted the importance of combining both physicochemical and genotypic characterization with spectroscopic analysis of the major natural products when isolating bioactive metabolites.

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Section: Discussionmentioning

confidence: 99%

Isolation and optimized production of putative antimicrobial compounds from Egyptian soil isolate Streptomyces sp. MS. 10

Sebak

Saafan

AbdelGhani

et al. 2021

Beni-Suef Univ J Basic Appl Sci

View full text Add to dashboard Cite

show abstract

“…C16 and C18 unsaturated fatty acids are more common in the plant kingdom. In addition, hydroxyl fatty acids with C16 and C18 chains are observed often, and have been reported to possess several biological activities such as anti-bacterial (Kurata et al 2010), anti-inflammatory (Masayuki et al 1998), and anti-melanogenesis effects (Fujita et al 2010).…”

Section: Resultsmentioning

confidence: 99%