On the Fueguian Atlantic coast and in the southern Andean fold and thrust belt, the Paleogene is partially represented by numerous turbiditic systems. Between Río Bueno and Cabo Leticia on Península Mitre, the Paleocene-Eocene is represented by the Río Claro Group (Cabo Leticia, La Barca, Punta Noguera and Cerro Ruperto formations) and the Río Bueno Formation; which together constitute a ~1050 m thickness succession. Here, calcareous nannofossil assemblages recovered from 96 samples were analyzed to determine their relative ages. The Cabo Leticia Formation was barren in nannofossils. The upper LB2 Member of La Barca Formation, contained Rhomboaster cuspis and Fasciculithus richardii, frequent in the Paleocene/Eocene boundary (Subzones NP9a/NP9b). Nannofossils recovered from Punta Noguera Formation indicate an early Eocene age (biozones NP9b-NP10), with Fascilulithus tympaniformis, Rhomboaster cuspis and abundant Toweius spp. The Cerro Ruperto Formation provided one productive sample, with specimens of Reticulofenestra spp. and Toweius spp., which indicates an early Eocene age for the formation (biozones NP12-NP13). The Río Bueno Formation yield calcareous nannofossil assemblages characterized by Chiasmolithus eograndis, Chiasmolithus expansus, Toweius spp. and Reticulofenestra spp. which indicates a late early Eocene to middle Eocene age (biozones NP14-NP16). Although the calcareous nannofossil record is discontinuous due to sedimentary facies of the Río Claro Group, our data allow a better age constraint for the investigated formations and to correlate these units with others outcropping and subsurface units in the Austral Basin.