Palm oil tocotrienol fractions restore endothelium dependent relaxation in aortic rings of streptozotocin-induced diabetic and spontaneously hypertensive rats

Muharis, Syed Putra; Top, Abdul Gapor Md; Murugan, Dharmani Devi; Mustafa, Mohd Rais

doi:10.1016/j.nutres.2010.03.005

Cited by 26 publications

(25 citation statements)

References 30 publications

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“…On the other hand, total content polyphenol of P. betle variety Kauri was 0.96 mg galic acid/mg. Furthermore, TRF consists of 85.6% inhibition in DPPH antioxidant assay compared to quercetin [59]. Relatively, C. vulgaris contained less antioxidants compared to P. betle and TRF.…”

Section: Discussionmentioning

confidence: 99%

Comparative effect of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction on antioxidant enzymes activity in cellular ageing of human diploid fibroblasts

Makpol

Yeoh

Ruslam

et al. 2013

BMC Complement Altern Med

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BackgroundHuman diploid fibroblasts (HDFs) undergo a limited number of cellular divisions in culture and progressively reach a state of irreversible growth arrest, a process termed cellular ageing. Even though beneficial effects of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction (TRF) have been reported, ongoing studies in relation to ageing is of interest to determine possible protective effects that may reverse the effect of ageing. The aim of this study was to evaluate the effect of P. betle, C. vulgaris and TRF in preventing cellular ageing of HDFs by determining the activity of antioxidant enzymes viz.; catalase, superoxide dismutase (SOD) and glutathione peroxidase.MethodsDifferent passages of HDFs were treated with P. betle, C. vulgaris and TRF for 24 h prior to enzymes activity determination. Senescence-associated beta-galactosidase (SA β-gal) expression was assayed to validate cellular ageing.ResultsIn cellular ageing of HDFs, catalase and glutathione peroxidase activities were reduced, but SOD activity was heightened during pre-senescence. P. betle exhibited the strongest antioxidant activity by reducing SA β-gal expression, catalase activities in all age groups, and SOD activity. TRF exhibited a strong antioxidant activity by reducing SA β-gal expression, and SOD activity in senescent HDFs. C. vulgaris extract managed to reduce SOD activity in senescent HDFs.ConclusionP. betle, C. vulgaris, and TRF have the potential as anti-ageing entities which compensated the role of antioxidant enzymes in cellular ageing of HDFs.

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Section: Discussionmentioning

confidence: 99%

Comparative effect of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction on antioxidant enzymes activity in cellular ageing of human diploid fibroblasts

Makpol

Yeoh

Ruslam

et al. 2013

BMC Complement Altern Med

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“…Medeiros et al [31] also demonstrated that the long-term intake of palm oil had beneficial effect in reducing blood pressure in spontaneously hypertensive rats. Free radical scavenging antioxidants in palm oil serve to protect endothelial cells against oxidative injury and thus improve endothelial functions [32]. Furthermore, the blood pressure lowering mechanism of palm oil may also involve the beneficial alteration in endothelium-derived factors [33].…”

Section: Discussionmentioning

confidence: 99%

Involvement of Inflammation and Adverse Vascular Remodelling in the Blood Pressure Raising Effect of Repeatedly Heated Palm Oil in Rats

Kamisah

Othman

et al. 2012

International Journal of Vascular Medicine

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Oil thermoxidation during deep frying generates harmful oxidative free radicals that induce inflammation and increase the risk of hypertension. This study aimed to investigate the effect of repeatedly heated palm oil on blood pressure, aortic morphometry, and vascular cell adhesion molecule-1 (VCAM-1) expression in rats. Male Sprague-Dawley rats were divided into five groups: control, fresh palm oil (FPO), one-time-heated palm oil (1HPO), five-time-heated palm oil (5HPO), or ten-time-heated palm oil (10HPO). Feeding duration was six months. Blood pressure was measured at baseline and monthly using tail-cuff method. After six months, the rats were sacrificed and the aortic arches were dissected for morphometric and immunohistochemical analyses. FPO group showed significantly lower blood pressure than all other groups. Blood pressure was increased significantly in 5HPO and 10HPO groups. The aortae of 5HPO and 10HPO groups showed significantly increased thickness and area of intima-media, circumferential wall tension, and VCAM-1 than other groups. Elastic lamellae were disorganised and fragmented in 5HPO- and 10HPO-treated rats. VCAM-1 expression showed a significant positive correlation with blood pressure. In conclusion, prolonged consumption of repeatedly heated palm oil causes blood pressure elevation, adverse remodelling, and increased VCAM-1, which suggests a possible involvement of inflammation.

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“…To the best of our knowledge, the present report represents the first study of an experimental model of simultaneous type 2 diabetes and renal hypertension. Previous studies on simultaneous models of hypertension and diabetes focused mainly on simultaneous type 1 diabetes and hypertension in spontaneous hypertensive rats (Hendriks et al 1993;Beenen et al 1996;Muharis et al 2010) and rats with renal hypertension (Mall et al 1987;Fischer et al 1992), and type 2 diabetes in spontanous hypertensive rats (Sato et al 1995;Ohtomo et al 2008) and rats with deoxycorticosteone acetate (DOCA)-saltinduced hypertension (Jadhav & Upasani 2011).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous renal hypertension and type 2 diabetes exacerbate vascular endothelial dysfunction in rats

Khalili

Nekooeian

Khosravi

et al. 2012

Int J Experimental Path

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Despite the high rate of occurrence of both diabetes and hypertension in humans, the cardiovascular effects of the two conditions have not been investigated when they occur simultaneously. Thus this study examined the vascular effects of simultaneous type 2 diabetes and renal hypertension on endothelial function. Serum malondialdehyde and systolic blood pressure (SBP) were measured, glucose tolerance test (GTT) was performed, and concentration-response to phenylephrine (PE) in the absence and presence of nitro-l-arginine methyl ester (l-NAME), acetylcholine and sodium nitroprusside were conducted on aortic rings from diabetic control, type 2 diabetes, sham-operated, renal hypertensive, and simultaneous type 2 diabetes plus hypertension rats respectively. Hypertension, diabetes, and simultaneous diabetes and hypertension were associated with either increased or decreased maximal responses (E(max)) of PE dependent on in the presence or absence of l-NAME. There was also increased serum malondialdehyde and decreased E(max) of acetylcholine. Thus simultaneous hypertension and diabetes caused a greater decrease in E(max) of acetylcholine compared to that seen with either diabetes or hypertension alone higher than that seen in hypertension. The blood glucose during GTT was lower than that seen in diabetes groups. Thus simultaneous type 2 diabetes and the SBP was renal hypertension is associated with improved glucose tolerance, but with further deterioration of endothelial dysfunction compared with either condition alone.

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Palm oil tocotrienol fractions restore endothelium dependent relaxation in aortic rings of streptozotocin-induced diabetic and spontaneously hypertensive rats

Cited by 26 publications

References 30 publications

Comparative effect of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction on antioxidant enzymes activity in cellular ageing of human diploid fibroblasts

Comparative effect of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction on antioxidant enzymes activity in cellular ageing of human diploid fibroblasts

Involvement of Inflammation and Adverse Vascular Remodelling in the Blood Pressure Raising Effect of Repeatedly Heated Palm Oil in Rats

Simultaneous renal hypertension and type 2 diabetes exacerbate vascular endothelial dysfunction in rats

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