Palmitic acid stimulates interleukin‐8 via the TLR4/NF‐κB/ROS pathway and induces mitochondrial dysfunction in bovine oviduct epithelial cells

Ohtsu, Ayaka; Tanaka, Hazuki; Seno, Kotomi; Iwata, Hisataka; Kuwayama, Takehito; Shirasuna, Koumei

doi:10.1111/aji.12642

Cited by 22 publications

(13 citation statements)

References 41 publications

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“…It may influence physiological functions of OECs, resulting in reduced OECs-sperm binding [9]. It was also hypothesized that raised fatty acid levels can lead to dysfunction and inflammation of OECs, causing infertility in bovine specimen [10].…”

Section: Introductionmentioning

confidence: 99%

Fatty Acids Related Genes Expression Undergo Substantial Changes in Porcine Oviductal Epithelial Cells During Long-Term Primary Culture

Budna

Celichowski

Knap

et al. 2018

Medical Journal of Cell Biology

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The process of reproduction requires several factors, leading to successful fertilization of an oocyte by a single spermatozoon. One of them is the complete maturity of an oocyte, which is acquired during long stages of folliculogenesis and oogenesis. Additionally, the oviduct, composed of oviductal epithelial cells (OECs), has a prominent influence on this event through sperm modification and supporting oocyte's movement towards uterus. OECs were isolated from porcine oviducts. Cells were kept in primary in vitro culture for 30 days. After 24h and on days 7, 15 and 30 cells were harvested, and RNA was isolated. Transcript changes were analyzed using microarrays. Fatty acids biosynthetic process and fatty acids transport ontology groups were selected for analysis and described. Results of this study indicated that majority of genes in both ontology groups were up-regulated on day 7, 15 and 30 of primary in vitro culture. We analyzed genes involved in fatty acids biosynthetic process, including: GGT1, PTGES, INSIG1, SCD, ACSL3, FADS2, FADS1, ACSS2, ALOX5AP, ACADL, SYK, ACACA, HSD17B8, FADS3, OXSM, and transport, including: ABCC2, ACSL4, FABP3, PLA2G3, PPARA, SYK, PPARD, ACACA and P2RX7. Elevated levels of fatty acids in bovine and human oviducts are known to reduce proliferation capacity of OECs and promote inflammatory responses in their microenvironment. Most of measured genes could not be connected to reproductive events. However, the alterations in cellular proliferation, differentiation and genes expression during in vitro long-term culture were significant. Thus, we can treat them as putative markers of changes in OECs physiology. Running title: Fatty acids changes in porcine OECs during in vitro culture

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Section: Introductionmentioning

confidence: 99%

Fatty Acids Related Genes Expression Undergo Substantial Changes in Porcine Oviductal Epithelial Cells During Long-Term Primary Culture

Budna

Celichowski

Knap

et al. 2018

Medical Journal of Cell Biology

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“…For example, elemol (Yang et al, 2015b) and thujopsene (Kim et al, 2013) decreased mast cell infiltration and IL-4 production. Palmitic acid has been shown to induce dendritic cell secretion of IL-1b (Nicholas et al, 2017) and activated production of IL-6, TNF-a (Zhou et al, 2013), and IL-8 (Ohtsu et al, 2017) by epithelial cells; 9-octadecenamide was suggested to have anti-inflammatory activities (Chen et al, 2011). It was also reported that terpenoid compounds may cause contact dermatitis (Mobacken and Fregert, 1975;Picman and Picman, 1990).…”

Section: Discussionmentioning

confidence: 99%

Skin Wound Healing Is Accelerated by a Lipid Mixture Representing Major Lipid Components of Chamaecyparis obtusa Plant Extract

Goleva

Hall

Park

et al. 2018

Journal of Investigative Dermatology

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In chronic nonhealing wounds, the healing process is disrupted and wounds are often infected with bacteria. About 85% of lower extremity amputations in diabetes are attributed to deep infection of foot ulcers. Therefore, infection control is critical for wound care. In this study, we analyzed lipid composition of Chamaecyparis obtusa extract, and we describe the wound-healing properties of its combination of 10 major lipid components. A 10-lipid mixture up-regulated HBD-3 and LL-37 through the olfactory receptor 2AT4 and induced phosphorylation of extracellular signal-regulated kinases and p38 mitogen-activated protein kinases in primary human keratinocytes. In addition, the 10-lipid mixture had direct bactericidal effects against Staphylococcus aureus and Streptococcus pyogenes and protected against staphylococcal α-toxin-induced keratinocyte cell death. In an animal model, the 10-lipid mixture accelerated skin wound healing and was also effective in healing wounds superinfected with S. aureus. We suggest that the 10-lipid mixture, because of its wound-healing and antimicrobial properties, can be beneficial for wound treatment.

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“…When the increased intracellular PA level exceeds its βoxidation in mitochondria, it is converted to harmful complex lipids such as diacylglycerin (DAG) and ceramide (5,27). These harmful FA-derived intermediates and excessive PA provoke increased reactive oxygen species (ROS) production, damage the function of mitochondria-related ER membrane (MAM), and induce mitochondrial fragmentation, resulting in mitochondrial dysfunction and loss of ATP production (28)(29)(30)(31). Recently, Rambold et al reported that fragmented mitochondria failed to efficiently take up FA, causing a reduction in β-oxidation rates and further exacerbating the accumulation of FA in Mitofusin1 KO cells (15).…”

Section: Discussionmentioning

confidence: 99%

Anethole dithiolethione improves liver fatty acid metabolism in hamster fed high-fat diets

Zhao

et al. 2020

Preprint

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Abstract“Lipotoxicity” induced by excessive accumulation of free fatty acids (FFAs) in the liver, especially saturated FAs and their toxic metabolites, is closely related to metabolic diseases such as nonalcoholic fatty liver disease (NAFLD). Hydrogen sulfide (H2S), a novel gaseous signaling molecule, has been reported to have lipid-lowering effects, but its effect on FAs metabolism remains unclear. The purpose of this study was to investigate the effect and mechanisms of anethole dithiolethione (ADT, a sustained-release H2S donor) on hepatic FAs metabolism. ADT was administered daily for 4 weeks in male Syrian golden hamsters fed a high fat diet (HFD), and FAs profiles of liver tissues were analyzed using GC-MS. The results showed that in HFD-fed hamsters, ADT treatment significantly reduced the accumulation of saturated and monounsaturated fatty acids (C16:0, C18:0, C16:1, and C18:1n9), while increased the content of n-6 and n-3 series polyunsaturated fatty acids (C20:3n6, C20:4n6, and C22:6n3). Mechanistically, ADT obviously inhibited the overexpression of ACC1, FAS and SCD1, and up-regulated the levels of FATPs, L-FABP, CPT1α, FADS1 and FADS2. Notably, ADT evidently induced Mitofusin1 to facilitate mitochondrial fusion and optimize β-oxidation. These findings suggest that ADT plays a beneficial role by regulating the synthesis, desaturation, β-oxidation, uptake, binding/isolation, and transport of FAs. In conclusion, ADT is effective in improving liver FAs metabolic disorders and liver injuries caused by HFD.

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Palmitic acid stimulates interleukin‐8 via the TLR4/NF‐κB/ROS pathway and induces mitochondrial dysfunction in bovine oviduct epithelial cells

Abstract: We suggest that bovine OECs recognize an excessive increase in endogenous and sterile danger signals, such as PA, which may contribute to chronic oviductal inflammation, resulting in infertility associated with oviductal dysfunction.

Cited by 22 publications

References 41 publications

Fatty Acids Related Genes Expression Undergo Substantial Changes in Porcine Oviductal Epithelial Cells During Long-Term Primary Culture

Fatty Acids Related Genes Expression Undergo Substantial Changes in Porcine Oviductal Epithelial Cells During Long-Term Primary Culture

Skin Wound Healing Is Accelerated by a Lipid Mixture Representing Major Lipid Components of Chamaecyparis obtusa Plant Extract

Anethole dithiolethione improves liver fatty acid metabolism in hamster fed high-fat diets

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