Palmitoleate enhances quality of rooster semen during chilled storage

Rad, Hamed; Eslami, Mohsen; Ghanie, Abolfazl

doi:10.1016/j.anireprosci.2015.12.003

Cited by 23 publications

(12 citation statements)

References 49 publications

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“…Both diluent and semen were at 37°C when mixed. Then, diluted semen was supplemented with 0 (control), 0.125 (P 0.125), 0.25 (P 0.25), 0.5 (P 0.5) and 1 (P 1) mM bovine serum albumin (BSA)‐conjugated palmitoleic acid (Rad et al., ). Following enrichment, the semen was stored for 72 hr at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Profitable effects of palmitoleic acid on AOA and signal transduction alteration have been documented in different cells (Dimopoulos, Watson, Sakamoto, & Hundal, ; Erbay, Babaev, & Mayers, ; Maedler, Oberholzer, Bucher, Spinas, & Donath, ; Sauma et al., ). Moreover, it has been demonstrated that palmitoleic acid would prevent the rooster spermatozoa from LPO during chilled storage (Rad, Eslami, & Ghanie, ). The present research was designated to investigate the effect of palmitoleic acid on motility (evaluated by CASA), viability and plasma membrane integrity of spermatozoa during chilled storage of ram semen.…”

Section: Introductionmentioning

confidence: 99%

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Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Eslami

Ghasemiyan

Hashem

2016

Reprod Domestic Animals

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The purpose of the present experiment was to investigate the protective effects of palmitoleate on the quality of ram semen during low temperature liquid storage. Ejaculates were collected using the artificial vagina from four Qezel rams twice a week. Ejaculates were pooled, diluted with Tris-egg yolk extender without palmitoleate (control) or supplemented with 0.125 (P 0.125), 0.25 (P 0.25), 0.5 (P 0.5) and 1 (P 1) mM palmitoleate at a final concentration of 500 × 10 spermatozoa/ml. Total motility and forward progressive motility (FPM) as well as other spermatozoa kinematics were evaluated by computer-assisted sperm analysis. Moreover, viability and membrane functionality were determined in the spermatozoa. Additionally, amounts of malondialdehyde (MDA), total antioxidant activity (AOA), nitric oxide (NO) and superoxide dismutase (SOD) activities were evaluated in the medium and spermatozoa at 0, 24, 48 and 72 hr of storage. The palmitoleate supplementation resulted in a significant (p < .05) increase in total motility and FPM with the highest increase at 0.5 mM concentration for 72 hr. P 0.5 group also resulted in the highest percentage of membrane-intact spermatozoa (76.60 ± 1.95%) and viability (75.81 ± 1.34%) at 72 hr (p < .05). The amounts of MDA and NO were lower in P 0.125, P 0.25 and P 0.5 groups compared to control at 48 hr and 72 hr (p < .05). Higher amounts of AOA were obtained in palmitoleate-treated groups in medium and spermatozoa during storage time (p < .05). Furthermore, palmitoleate supplementation increased the SOD activities in spermatozoa compared to the control (p < .05). The results of the present experiment reveal that supplementation with 0.5 mM palmitoleate improves ram spermatozoa motion characteristics, AOA levels and SOD activities during liquid storage. Then, palmitoleate could be used as an antioxidant source during liquid storage of ram semen.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Eslami

Ghasemiyan

Hashem

2016

Reprod Domestic Animals

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“…It seems that antioxidative action of additives would be affected by type and the doses of antioxidant compounds, duration of treatment, the animal species, method of administration (Lima et al, 2001;Mirzaei Rad et al, 2016;Sikka, 2004;Taepongsorat, Tangpraprutgul, Kitana, & Malaivijitnond, 2008) and the longevity of produced ROS (Sies, 1993).…”

Section: Discussionmentioning

confidence: 99%

Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C

Ghaniei

Eslami

Hashem

et al. 2019

Animal Physiology Nutrition

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Current study was carried out to examine the protective effects of quercetin against toxicity induced by hydrogen peroxide in rooster semen in vitro. Semen samples were collected from ten roosters (Ross 308 broiler breeder males, 32 weeks old) twice a week by abdominal massage method. Samples with ≥70% progressive motility were selected, pooled, diluted and used for the study. Experimental groups consisted of negative control, control that received solvent of quercetin, H2O2 (40 μM) and combination groups which incubated with constant dose of H2O2 (40 μM) plus various levels of quercetin (20, 40 and 80 μM). Measurement of total hydroperoxide (HPO), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC) and superoxide dismutase activity as well as routine sperm tests were done at 0, 24 and 48 hr of storage at 4°C. Results revealed that exposure to hydrogen peroxide significantly increased HPO (138.43 ± 7.32 vs. 66.08 ± 3.97 μmol/g protein), MDA (7.21 ± 0.08 vs. 5.71 ± 2.16 μmol/g protein) and NO (0.367 ± 0.013 vs. 0.215 ± 0.011 μmol/g protein) levels and decreased sperm progressive motility (27.28 ± 1.21 vs. 47.49 ± 1.29%), and amounts of TAC (11.49 ± 0.39 vs. 15.70 ± 0.79 mmol/g protein) compared to control at 24 hr (p < 0.05). Changes at mentioned variables were repeated at 48 hr of storage. Also, co‐administration of quercetin (especially at 40 and 80 μM) with hydrogen peroxide restored the toxic effects of hydrogen peroxide on rooster semen parameters such as primary and secondary lipid peroxidative indicators and other evaluated variables. The study concluded that rooster semen enrichment with quercetin would protect lipid peroxidative and nitrosative hydrogen peroxide‐mediated damage during cold liquid storage of rooster semen.

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“…Although we have not yet had the opportunity to test this hypothesis directly in crocodile spermatozoa, we did secure several lines of correlative evidence that these cells use a similar metabolic strategy. Thus, capacitated crocodile spermatozoa experienced a selective depletion of palmitoleic acid [a monounsaturated fatty acid substrate known to enhance the motility profile of spermatozoa from species as diverse as sheep and fowls (35,36)], as well as a significant reduction in motility following the uncoupling of oxidative phosphorylation. Moreover, we identified CPT1A as a substrate for differential phosphorylation in noncapacitated versus capacitated crocodile spermatozoa.…”

Section: Molecular and Cellular Proteomics 1813 S73mentioning

confidence: 99%

Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals*

Nixon¹,

Johnston²,

Skerrett‐Byrne³

et al. 2019

Molecular & Cellular Proteomics

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Competition to achieve paternity has contributed to the development of a multitude of elaborate male reproductive strategies. In one of the most well-studied examples, the spermatozoa of all mammalian species must undergo a series of physiological changes, termed capacitation, in the female reproductive tract prior to realizing their potential to fertilize an ovum. However, the evolutionary origin and adaptive advantage afforded by capacitation remains obscure. Here, we report the use of comparative and quantitative proteomics to explore the biological significance of capacitation in an ancient reptilian species, the Australian saltwater crocodile (Crocodylus porosus). Our data reveal that exposure of crocodile spermatozoa to capacitation stimuli elicits a cascade of physiological responses that are analogous to those implicated in the functional activation of their mammalian counterparts. Indeed, among a total of 1119 proteins identified in this study, we detected 126 that were differentially phosphorylated (± ≥1.2 fold-change) in capacitated versus non-capacitated crocodile spermatozoa. Notably, this subset of phosphorylated proteins shared substantial evolutionary overlap with those documented in mammalian spermatozoa, and included key elements of signal transduction, metabolic and cellular remodeling pathways. Unlike mammalian sperm, however, we noted a distinct bias for differential phosphorylation of serine (as opposed to tyrosine) residues, with this amino acid featuring as the target for ~80% of all changes detected in capacitated spermatozoa. Overall, these results indicate that the phenomenon of sperm capacitation is unlikely to be restricted to mammals and provide a framework for understanding the molecular changes in sperm physiology necessary for fertilization.

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Palmitoleate enhances quality of rooster semen during chilled storage

Cited by 23 publications

References 49 publications

Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C

Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals*

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Palmitoleate enhances quality of rooster semen during chilled storage

Cited by 23 publications

References 49 publications

Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Semen supplementation with palmitoleic acid promotes kinematics, microscopic and antioxidative parameters of ram spermatozoa during liquid storage

Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4°C

Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals*

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Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C