Palmitoylation of Tubulin

Phosphatidylinositol 4,5-Bisphosphate Modifies Tubulin Participation in Phospholipase Cβ1Signaling

“…The recently discovered microtubule depolymerization and translocation of tubulin from the cytosol to the membrane in response to receptor stimulation showed one mechanism for tubulin targeting to the membrane (Popova and Rasenick, 2000;Ciruela and McIlhinney, 2001; this study). The finding that tubulin is posttranslationally palmitoylated (Caron, 1997;Zambito and Wolff, 1997) supports this observation, be- M) was added, the samples were incubated for 30 min at 37°C, and the total inositol phosphate production was measured as described. **Significantly different from control cells ( p Ͻ 0.01); *significantly different from carbachol-treated cells ( p Ͻ 0.05).…”

The Tubulin Code

“…51 In addition, microinjection of antibody GT335 that recognizes mono-and polyglutamylated tubulins caused a transient disappearance of centrioles and spindle defects. 113 Palmitoylation occurs on Cys376 of vertebrate a-tubulin 131,132 and mutation of the corresponding Cys377 residue to serine in yeast affected aspects of mitosis that involve interactions of astral microtubules with the cell cortex such as translocation of spindles to the bud. 133 Thus, palmitoylation of astral microtubules could tether spindle microtubules to the plasma membrane.…”

Low expression of human tubulin tyrosine ligase and suppressed tubulin tyrosination/detyrosination cycle are associated with impaired neuronal differentiation in neuroblastomas with poor prognosis

“…Tubulin is subjected to several types of evolutionarily conserved posttranslational modification that includes tyrosination/detyrosination, acetylation, phosophorylation, palmitoylation, polyglutamylation and polyglycylation. [1][2][3][4] The discovery of tyrosination cycle stems from the serial observations that the addition of radiolabeled tyrosine to a rat brain cytosolic extract leads to tyrosination of the COOH terminus of a single endogenous protein, ␣-tubulin, by a translation-independent mechanism. [5][6][7] Posttranslational incorporation of tyrosine into the tubulin has also been shown to occur in vivo.…”