Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy

Abstract

Palyno‐anatomical study of monocots taxa using Light and Scanning Electron Microscopy (SEM) was first time conducted with a view to evaluating their taxonomic significance. Studied plants were collected from different eco‐climatic zones of Pakistan ranges from tropical, sub‐tropical, and moist habitats. The aim of this study is to use palyno‐anatomical features for the correct identification, systematic comparison, and investigation to elucidate the taxonomic significance of these features, which are useful to taxonomists for identifying monocot taxa. A signification variation was observed in quantitative and qualitative characters by using the standard protocol of light microscopy (LM) and SEM. Epidermal cell length varied from maximum in Allium griffthianum (480 ± 35.9) μm at the adaxial surface to minimum in Canna indica (33.6 ± 8.53) μm on abaxial surface. Maximum exine thickness was observed in Canna indica (4.46) μm and minimum in Allium grifthianum (0.8) μm. Variation was observed in shape and exine ornamentation of the pollen, shape of the epidermal cell, number, size, and type of stomata, guard cell shape, and anticlinal wall pattern. Based on these palyno‐anatomical features a taxonomic key was developed, which help in the discrimination of studied taxa. In conclusion, LM and SEM pollen and epidermal morphology is explanatory, significant, and can be of special interest for the plant taxonomist in the correct identification of monocots taxa.

Taxonomic significance of cypsela morphology in tribe Cichoreae (Asteraceae) using light microscopy and scanning electron microscopy

“…This observation matches with that previously reported by Abid and Qaiser (). Similarly, micromorphology of plant materials observed under SEM of various plants groups that is, monocots (Bahadur et al, , ) and other dicotyledon families (Ahmad et al, ). Foliar micromorphology (Ashfaq et al, ; Gul et al, ) and morphology of nanoparticle (Saqib et al, ) have been proved considerably in the correct identification of species.…”

Antimicrobial Activity and Phytochemical Screening of Euphorbia helioscopia

“…Further taxonomic study of the is recommended for the correct identification. Micromorphology of plants material have been proven significant in correct identification and discrimination of the species Ashfaq et al, 2018;Ashfaq et al, 2019b;Bahadur et al, 2018b;Bahadur et al, 2019;Gul et al, 2019a;Gul et al, 2019b;Gul et al, 2019c;Naz et al, 2019;Sufyan et al, 2018;Bano et al, 2019;Nazish et al, 2019;Qureshi et al, 2019;Arshad et al, 2019;Rashid et al, 2018). Therefore, they can be utilized in the treatment of infectious diseases caused by resistant microbes.…”

Taxonomic study of one generic and two new species record to the flora of Pakistan using multiple microscopic techniques

“…Previous studies adopted microscopic techniques for addition of new species record, plant systematics, anatomical, micro, and macro morphological studies and finding variations among medicinal plants authenticity (Bahadur et al, ; Bahadur et al, ; David, Ain, Ahmad, Zaman, & Jahan, ; Esfandani‐Bozchaloyi & Zaman, ; Raei Niaki et al, ; Shah, Ahmad, Zafar, & Zaman, ; Shah et al, ; Shah et al, ; Shah et al, ; Shah et al, : Ullah et al, ). To introduce new species in Pakistan flora, the taxonomist and scientists contributed in this regard.…”

“…Previous studies adopted microscopic techniques for addition of new species record, plant systematics, anatomical, micro, and macro morphological studies and finding variations among medicinal plants authenticity Bahadur et al, 2019;David, Ain, Ahmad, Zaman, & Jahan, 2019;Esfandani-Bozchaloyi & Zaman, 2018;Raei Niaki et al, 2019;Shah et al, 2019a;Shah et al, 2019b;Shah et al, 2019 anomocytic type and oval shape. Nonglandular trichomes (NGTs) were three cells long, whereas glandular trichomes were noted as capitate glandular trichomes at the abaxial surface ( Figure 3).…”