Pancreas-protective effects of chlorella in STZ-induced diabetic animal model: insights into the mechanism

Amin, Amr; Lotfy, Mohamed; Mahmoud‐Ghoneim, Doaa; Adeghate, Ernest; Al-Akhras, Mohamed A.; Al-Saadi, Maryam; Al-Rahmoun, Salam; Hameed, Rasheed

doi:10.4236/jdm.2011.13006

Cited by 54 publications

(16 citation statements)

References 30 publications

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“…The substantial drop in HOMA-B supports the deterioration of β cell function in animal models as lower HOMA-B index reflect the failure of pancreatic β-cell function [ 37 ]. This is in agreement with studies demonstrating that a single dose of 60 mg/kg STZ is capable to induce pancreatic β-cell destruction in rats and subsequent reduction of insulin secretion [ 38 , 39 ]. One particular interesting finding was that DFD and DV rats had increased the size and density of dispersed islet tissue (Fig.…”

Section: Discussionsupporting

confidence: 92%

Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin

Samsulrizal

Goh

Ahmad

et al. 2017

BMC Complement Altern Med

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BackgroundThe potential application of Ficus deltoidea and vitexin for the management of symptomatologies associated with diabetes mellitus (DM) has gained much attention. However, less firm evidence comes from data to augment our understanding of the role of F. deltoidea and vitexin in protecting pancreatic β-cells. The aim of this study was to assess histological and oxidative stress changes in the pancreas of streptozotocin (STZ)-induced diabetic rats following F. deltoidea extract and vitexin treatment.Methods F. deltoidea and vitexin was administrated orally to six-weeks STZ-induced diabetic rats over 8 weeks period. The glucose and insulin tolerances were assessed by intraperitoneal glucose (2 g/kg) tolerance test (IPGTT) and intraperitoneal insulin (0.65 U/kg) tolerance test (IPITT), respectively. Subsequently, insulin resistance was assessed by homeostasis assessment model of insulin resistance (HOMA-IR), quantitative insulin sensitivity check index (QUICKI) and the insulin/triglyceride-derived McAuley index. The histological changes in the pancreas were then observed by hematoxylin-eosin (H&E) staining. Further, the pattern of fatty acid composition and infrared (IR) spectra of the serum and pancreas were monitored by gas chromatography (GC) method and Fourier Transform Infrared (FT-IR) spectroscopy.Results F. deltoidea and vitexin increased pancreatic antioxidant enzymes and promoted islet regeneration. However, a significant increase in insulin secretion was observed only in rats treated with F. deltoidea. More importantly, reduction of fasting blood glucose is consistent with reduced FT-IR peaks at 1200-1000 cm−1.ConclusionsThese results accentuate that F. deltoidea and vitexin could be a potential agent to attenuate pancreatic oxidative damage and advocate their therapeutic potential for treating DM.

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Section: Discussionsupporting

confidence: 92%

Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin

Samsulrizal

Goh

Ahmad

et al. 2017

BMC Complement Altern Med

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“…16 , 17 The natural products continue to play an important role in drug discovery programs for different diseases. Using different technical approaches, 18 , 19 studies showed the effectiveness of these components for various disorders such as carcinomas, 20 , 21 , 22 , 23 , 24 , 25 , 26 diabetes, 27 various inflammatory and fibrotic conditions. 28 , 29 , 30 , 31 , 32 One of these natural products was isolated from Crocus sativus, a flowering plant in the Iridaceae family and is commonly known as saffron.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of active components of saffron in post-surgical adhesion band formation

Arjmand

Hashemzehi

Soleimani

et al. 2021

Journal of Traditional and Complementary Medicine

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Background Abdominal adhesions are common and often develop after abdominal surgery. There are currently no useful targeted pharmacotherapies for adhesive disease. Saffron and its active constituents, Crocin and Crocetin, are wildly used in traditional medicine for alleviating the severity of inflammatory or malignant disease. Purpose The aim of this study was to investigate the therapeutic potential of the pharmacological active component of saffron in attenuating the formation of post-operative adhesion bands using different administration methods in a murine model. Material method saffron extract (100 mg/kg), Crocin (100 mg/kg), and Crocetin (100 mg/kg) were administered intraperitoneally and by gavage in various groups of male Wistar rat post-surgery. Also three groups were first treated intra-peritoneally by saffron extract, Crocin, and Crocetin (100 mg/kg) for 10 days and then had surgery. At the end of the experiments, animals sacrificed for biological assessment. Result A hydro-alcoholic extract of saffron and crocin but not crocetin potently reduced the adhesion band frequency in treatment and pre-treatment groups in the mice given intra-peritoneal (i.p) injections. Following the saffron or crocin administration, histological evaluation and quantitative analysis represented less inflammatory cell infiltration and less collagen composition, compared to control group. Moreover, the oxidative stress was significantly reduced in treatment groups. Conclusion These findings suggest that a hydro-alcoholic extract of saffron or its active compound, crocin, is a potentially novel therapeutic strategy for the prevention of adhesions formation and might be used as beneficial anti-inflammatory or anti-fibrosis agents in clinical trials. Taxonomy Abdominal surgeries/post-surgical adhesions.

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“…ROS, superoxide radicals and hydrogen peroxide are all produced during pro-inflammatory b-cell attack and glycolipid-toxicity-mediated b-cell dysfunction (Li et al 2008). In combination with nitric oxide accumulation, these processes lead to the generation of more ROS resulting in pancreatic b-cell death (Amin et al 2011).…”

Section: Effect Of Exenatide On Endogenous Antioxidantsmentioning

confidence: 99%

Mechanism of the beneficial and protective effects of exenatide in diabetic rats

Lotfy

Singh

Rashed

et al. 2013

Journal of Endocrinology

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Glucagon-like peptide 1 (GLP1) agonists are promising therapeutic agents in the treatment of diabetes mellitus. This study examines the mechanism of the protective effects of exenatide in experimental diabetes, employing four groups of ten rats each, in which two groups were streptozotocin-induced diabetic and two were control groups. One control and one diabetic group were treated with exenatide (1 mg/kg body weight (BW)) for 10 weeks. Blood plasma was taken for biochemical analyses while pancreatic tissue was taken for immunofluorescence and immunoelectron microscopy studies and real-time PCR to examine the expression of genes. The results show that exenatide improved BW gain and reduced blood glucose in diabetic rats compared with controls. Similarly, exenatide enhanced insulin release from the pancreatic fragments and improved liver and kidney functions and lipid profile in diabetic rats compared with controls. Exenatide not only induced significant increases in serum insulin level but also elevated the number of insulin-, GLP1-and exenatide-positive cells compared with untreated controls. Exenatide also elevated the number of catalase-and glutathione reductase-positive cells in diabetic rat pancreas compared with controls. Exenatide caused significant elevation in the expressions of pancreatic duodenal homeobox-1, heat shock protein-70, glutathione peroxidase, insulin receptor and GLP1 receptor genes in the pancreas of both control and diabetic rats compared with untreated animals. The results have demonstrated that exenatide can exert its beneficial and protective effects by elevating the levels of endogenous antioxidants and genes responsible for the survival, regeneration and proliferation of pancreatic b-cell.

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Pancreas-protective effects of chlorella in STZ-induced diabetic animal model: insights into the mechanism

Cited by 54 publications

References 30 publications

Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin

Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin

Therapeutic potential of active components of saffron in post-surgical adhesion band formation

Mechanism of the beneficial and protective effects of exenatide in diabetic rats

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