Pancreatic acinar cells: ionic dependence of the membrane potential and acetylcholine‐induced depolarization

Matthews, E. K.; Petersen, O. H.

doi:10.1113/jphysiol.1973.sp010233

Cited by 88 publications

(59 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We attribute this to improvement in technique. The mean resting membrane potential measured in twenty experiments during superfusion with standard Tris buffered Ringer solution was -43-8 + 0-8 mV, which is similar to that found using KHB and more negative than the value reported by Matthews & Petersen (1973) of -35 mV.…”

Section: Resting Membrane Potentialsmentioning

confidence: 48%

“…3) the studies were complicated PANCREATIC MEMBRANE POTENTIALS A23187-induced depolarization raised the question of whether the Ca2+ dependent depolarization might be due not to an increased divalent cation conductance but to an effect of the ionophore-mediated increased concentration of intracellular free Ca2+ on the permeability of the cell membrane to other ions. It has been concluded that the depolarization of the acinar cell membrane in response to ACh or pancreozymin is due to an increased permeability to Na+ (Matthews & Petersen, 1973;Nishiyama & Petersen, 1974. Consequently, the possibility that a secondary, Ca2+-dependent increase in the permeability to Na+ was involved in the depolarization by A23187 was tested.…”

Section: Ionic Dependence Of A23187-induced Depolarizationmentioning

confidence: 99%

“…Electrophysiological techniques have recently been applied to pancreatic acinar cells as a means of studying the earliest membrane-related steps in stimulus-secretion coupling (Dean & Matthews, 1972;Kanno, 1972;Matthews & Petersen, 1973;Greenwell, 1975). Nishiyama & Petersen (1974 have shown that depolarization induced by cholinergic agonists can be explained as due to an increased membrane permeability to Na+, possibly associated with a smaller increase in the permeability to K+.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Effects of the calcium ionophore A23187 on pancreatic acinar cell membrane potentials and amylase release.

Poulsen¹,

Williams²

1977

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The effects of the Ca2+-ionophore A23187 and the non-metabolizable cholinergic agonist bethanechol on acinar cell membrane potentials and amylase release from the superfused mouse pancreas were studied.2. In the presence of extracellular Ca2+ (2.56 mM), A23187 (10-5M) and bethanechol (3 x 10-, M) caused an equal increase in the release of amylase. Both stimulants depolarized the acinar cells, A23187 by 60 mV and bethanechol by 12-3 mV.3. When Ca2+ and Mg2+ were removed from the superfusate, the ability of A23187 to increase the rate of amylase release was virtually abolished, while the effect of bethanechol remained unaltered. Similarly, in the absence of these divalent cations, A23187 did not cause depolarization of the acinar cells, while depolarization in response to bethanechol was largely normal. Consequently it is unlikely that cholinergic agonists initiate secretion by activating a Ca2+-ionophore-like mechanism in the cell membrane.4. When the concentration of Ca2+ in the medium was raised to 10 mM, as the only divalent cation present, the depolarization in response to A23187 was increased to 11-8 mV. When Mg2+ in a concentration of 10 mM was the only extracellular divalent cation, the depolarization was only 2-1 mV.5. The Ca2+ dependent, A23187-induced depolarization was abolished in the absence of Na+ (Tris substitution). Addition of Na+ to the superfusate caused an immediate depolarization.6. It is concluded that the Ca2+ dependent depolarization of pancreatic acinar cells induced by A23187 is not directly due to an increased divalent

show abstract

Section: Resting Membrane Potentialsmentioning

confidence: 48%

Section: Ionic Dependence Of A23187-induced Depolarizationmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Effects of the calcium ionophore A23187 on pancreatic acinar cell membrane potentials and amylase release.

Poulsen¹,

Williams²

1977

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…This has been shown for acetylcholine (ACh) (Matthews & Petersen, 1973; Nishiyama & Petersen, 1974), cholecystokinin pancreozymin (CCK-PZ) and its relatives caerulein (Iwatsuki, Kato & Nishiyama, 1977) and gastrin-like peptides (Petersen & Ueda, 1975;Iwatsuki et al 1977). The electrical effects of bombesin on acinar cells have also been demonstrated more recently (Jwatsuki & Petersen, 1978).…”

Section: Introductionmentioning

confidence: 90%

Pancreatic acinar cells: effects of microionophoretic polypeptide application on membrane potential and resistance

Petersen

Philpott

1979

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

SUMMARY1. Acinar cell membrane potential and resistance were measured from superfused segments of mouse pancreas, in vitro, using intracellular glass micro-electrodes. One or two extracellular micropipettes containing caerulein, bombesin nonapeptide (Bn) or acetylcholine (ACh) were placed near to the surface of the impaled acinus. The secretagogues were ejected rapidly from the micropipettes by ionophoresis.2. Each secretagogue evoked a similar electrical response from the impaled acinar cell: membrane depolarization and a simultaneous reduction in input resistance. The duration of cell activation from caerulein ionophoresis was longer than that observed for ACh and Bn. The cell response to the peptide hormone applications could be repeated in the presence of atropine.3. The minimum interval before the onset of cell depolarization after caerulein ionophoresis was determined. Values ranged between 500 and 1000 msec. The minimum latencies after Bn ionophoresis were 500-1400 msec.4. With two electrodes inserted into electrically coupled acinar cells, direct measurements of the caerulein and Bn null potentials were made. At high negative membrane potentials an enhanced depolarization was evoked by caerulein ionophoresis. At low negative membrane potentials the caerulein stimulation produced a diminished depolarization, and at membrane potentials less than -10 mV acinar cell hyperpolarizations were observed. A similar series of responses was obtained in experiments where Bn ionophoresis was used. The caerulein and the Bn null potentials were always contained within -10 to -15 mV.5. The results describe the almost identical electrical response of acinar cells to stimulation by ACh, caerulein and bombesin. All three secretagogues have similar null potentials and latencies of activation on acinar cells. The bombesin latency responses appear as short as those measured for caerulein and provide electrophysiological evidence that Bn acts directly on acinar cells. The findings support the hypothesis that ACh, caerulein and Bn, though acting on different receptors, evoke the observed changes in electrical properties of acinar cell membranes, through a common pathway.

show abstract

“…Similar results were obtained in Cl-free isethionate solution where EAch was shifted to -1.4+0.7 mV (Fig.9). If we assume that the values for intracellular Na and K ion activities found (Nishiyama and Petersen 1974) (slope of 37 mV decade) than to the result in the mouse pancreas where the slope was found to be only 28 mV decade (Matthews and Petersen 1973).…”

Section: Resting Membrane Potential and Input Resistancementioning

confidence: 99%

Fluid secretion and electrical properties of pancreatic acini of Syrian Golden hamster.

Suzuki

1986

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

SuzuKI, Y. Fluid Secretion and Electrical Properties of Pancreatic Acini of Syrian Golden Hamster. Tohoku J. exp. Med., 1986, 150 (2), [189][190][191][192][193][194][195][196][197][198][199][200][201][202][203][204][205][206][207][208] Mechanisms of electrolyte and fluid secretion in the hamster pancreatic acini were investigated by measurements of fluid and amylase secretion in vivo and in vitro, and membrane potential and resistance in vitro. Unlike mouse and rat pancreas the hamster pancreas secreted only a small amount of juice in response to acinar stimulants such as acetylcholine (ACh) and caerulein. The mean resting potential was -62 mV and the mean resting input resistance was 29 M2. Ionophoretic application of ACh evoked membrane depolarization accompanied by a reduction in input resistance. The reversal potential for this ACh-evoked depolarization (EAch) was -11 mV. The EAch was shifted by changing extracellular K, Na and Cl concentration in a similar way to that of mouse pancreatic acinar cells. The relative permeability and current for K, Na and Cl at the EAch calculated by using the Goldman-Hodgkin-Katz equations were PK/PNa/Pc, =1/0.9/0.43 and IK/INa/ Ic, =1/1.4/0.4 respectively (In the mouse Pk/PNa/Pc, =1/1.2/5 and IK/INa/Ic, =1/ 2.3/1.3, Petersen et al. 1981). The results indicate that mechanisms of electrolyte and fluid secretion in hamster pancreatic acini are qualitatively similar to those in the mouse and rat pancreas. The less permeability of the acinar cell membrane to Cl ions in the hamster than in the mouse and rat appears to be associated with the fact that the acinar stimulant evokes a far smaller amount of fluid secretion in this species than in the mouse and rat.hamster ; pancreatic acinar cells ; fluid secretion ; electrical properties ; membrane permeabilityIn response to secretagogues such as acetylcholine (ACh) and pancreozymin pancreatic acinar cells secrete not only enzymes but also fluid with various secretory rate in different species. Species can be classified into two groups with respect to the degree of fluid secretion ; the first group includes the rat (Petersen and Ueda 1977) and mouse (Mangos et al. 1973) in which the pancreas secretes enzymes together with a large amount of pancreatic juice, and the second group includes the cat (Case et al. 1969) and man (Rinderknecht et al. 1978) in which the pancrease secretes enzyme with little juice.Electrical properties of pancreatic acinar cells can be also classified into two groups with respect to the response to secretagogues. In the mouse and rat

show abstract

Pancreatic acinar cells: ionic dependence of the membrane potential and acetylcholine‐induced depolarization

Cited by 88 publications

References 25 publications

Effects of the calcium ionophore A23187 on pancreatic acinar cell membrane potentials and amylase release.

Effects of the calcium ionophore A23187 on pancreatic acinar cell membrane potentials and amylase release.

Pancreatic acinar cells: effects of microionophoretic polypeptide application on membrane potential and resistance

Fluid secretion and electrical properties of pancreatic acini of Syrian Golden hamster.

Contact Info

Product

Resources

About