Pancreatic cancer: from bench to bedside

Ma, Yaokai; Wu, Qing; Li, Xin; Gu, Xing; Xu, Jiahua; Yang, Jian

doi:10.21037/atm.2016.11.57

Cited by 13 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Study subjects. A total of 42 patients (25 male, 17 female; aged 31-77 years) who were pathologically diagnosed with PC and underwent exairesis (28) between November, 2013 and November, 2016 at the First Affiliated Hospital of Dalian Medical University (Dalian, China) were included in this study. The inclusion criteria were as follows: i) Patients who were pathologically confirmed to suffer from PC with complete clinical data; ii) patients who had no history of pancreatitis, radiochemotherapy or pancreatic surgery prior to the exairesis; iii) patients who successfully underwent surgery with no complications.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-494 acts as a tumor suppressor in pancreatic cancer, inhibiting epithelial-mesenchymal transition, migration and invasion by binding to SDC1

Yang

Xiong

et al. 2018

Int J Oncol

View full text Add to dashboard Cite

Pancreatic cancer (PC) is the fourth most common cause of cancer‑related mortality in the industrialized world. Emerging evidence indicates that a variety of microRNAs (miRNAs or miRs) are involved in the development of PC. The aim of the present study was to elucidate the mechanisms through which miR‑494 affects the epithelial‑mesenchymal transition (EMT) and invasion of PC cells by binding to syndecan 1 (SDC1). PC tissues and pancreatitis tissues were collected, and the regulatory effects of miR‑494 on SDC1 were validated using bioinformatics analysis and a dual‑luciferase report gene assay. The cell line with the highest SDC1 expression was selected for use in the following experiments. The role of miR‑494 in EMT was assessed by measuring the expression of SDC1, E‑cadherin and vimentin. Cell proliferation was assessed using a cell counting kit (CCK)‑8 assay, migration was measured using a scratch test, invasion was assessed with a Transwell assay and apoptosis was detected by flow cytometry. Finally, a xenograft tumor model was constructed in nude mice to observe tumor growth in vivo. We found that SDC1 protein expression was significantly higher in the PC tissues. SDC1 was verified as a target gene of miR‑494. The SW1990 cell line was selected for use in further experiments as it had the lowest miR‑494 expression and the highest SDC1 expression. Our results also demonstrated that miR‑494 overexpression and SDC1 silencing significantly decreased the mRNA and protein expression of SDC1 and vimentin in SW1990 cells, while it increased E‑cadherin expression and apoptosis, and inhibited cell growth, migration, invasion and tumor growth. On the whole, the findings of this study demonstrated that miR‑494 is able to downregulate SDC1 expression, thereby inhibiting the progression of PC. These findings reveal a novel mechanism through which miR‑494 affects the development of PC and may thus provide a basis for the application of miR‑494 in pancreatic oncology.

show abstract

Section: Methodsmentioning

confidence: 99%

MicroRNA-494 acts as a tumor suppressor in pancreatic cancer, inhibiting epithelial-mesenchymal transition, migration and invasion by binding to SDC1

Yang

Xiong

et al. 2018

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…Only 5% to 10% of patients are candidates for potentially curative resection at the time of diagnosis. [6] From this perspective, a throughout understanding of this lethal disease targeting early detection is required. Intra-tumor hemodynamics, or tumor perfusion, provides useful information in understanding pathological background of cancers.…”

Section: Introductionmentioning

confidence: 99%

The application of high-field magnetic resonance perfusion imaging in the diagnosis of pancreatic cancer

Yang

Zhang²,

Ren³

2017

Medicine

View full text Add to dashboard Cite

Pancreatic cancer is the fourth leading cause of cancer death in the world. It is a disease of insidious progression and high lethality. The present study was to investigate the diagnostic value of high-filed magnetic resonance (MR) perfusion imaging in pancreatic cancer. Thirty-three patients with suspected pancreatic cancer were recruited in our study and underwent routine MR imaging. When compared with para-tumoral and normal tissue, the pancreatic lesions showed significant lower slope, peak enhancement (PE), and signal enhancement ratio (SER) as well as higher time to peak (TTP). Para-tumoral tissue was found to have significantly lower slope and PE, slightly higher TTP than normal tissue. MR perfusion imaging displays hemodynamic alterations in both pancreatic cancer and surrounding pancreatic tissue, and provides indirect assessment of tumor vascularity. In conclusion, high field MR perfusion imaging has important clinical significance in early diagnosis of pancreatic cancer.

show abstract

“…In the case of pancreatic cancer, early stage disease detection and the development of new treatment strategies have previously been identified as priorities eight years ago. 19 Progress within these domains has since been made, however they may remain high priorities due to difficulties in translating basic research to bedside 20 and persistent complexities within surgical research. 21 A national quality improvement programme for iatrogenic common bile duct injury was sufficiently prioritised for consideration in the final group of questions (Supplementary Table 2), however nearly a quarter of respondents (24%) ranked this question as low priority (grade 1 or 2).…”

Section: Discussionmentioning

confidence: 99%

Use of a modified Delphi approach to develop research priorities in HPB surgery across the United Kingdom

Knight

Pathak²,

Christie

et al. 2019

HPB

View full text Add to dashboard Cite

Background: Research prioritisation can help identify clinically relevant questions and encourage highquality, patient-centred research. Delphi methodology aims to develop consensus opinion within a group of experts, with recent Delphi projects helping to define the research agenda and funding within several medical and surgical specialties. Methods: All members of the Association of Upper Gastrointestinal Surgeons (AUGIS) were asked to submit clinical research questions using an online survey (Phase 1). Two consecutive rounds of Delphi prioritisation by multidisciplinary HPB healthcare professionals (Phase 2) were undertaken to establish a final list of the most highly prioritised research questions. A multidisciplinary steering committee analysed the results of each phase. Results: Ninety-three HPB-focussed questions were identified in Phase 1, with thirty-seven questions of sufficient priority to enter a further prioritisation round. A final group of 11 questions considered highest priority were identified. The most highly ranked research questions related to treatment pathways, operative strategies and the impact of HPB procedures on quality of life, particularly for malignant disease. Conclusion: Expert consensus has identified research priorities within the UK HPB surgical community over the coming years. Funding applications, to establish well-designed, high quality collaborative research are now required to address these questions.

show abstract

Pancreatic cancer: from bench to bedside

Cited by 13 publications

References 31 publications

MicroRNA-494 acts as a tumor suppressor in pancreatic cancer, inhibiting epithelial-mesenchymal transition, migration and invasion by binding to SDC1

MicroRNA-494 acts as a tumor suppressor in pancreatic cancer, inhibiting epithelial-mesenchymal transition, migration and invasion by binding to SDC1

The application of high-field magnetic resonance perfusion imaging in the diagnosis of pancreatic cancer

Use of a modified Delphi approach to develop research priorities in HPB surgery across the United Kingdom

Contact Info

Product

Resources

About