Pancreatic enzymes in the epithelium of intrahepatic large bile ducts and in hepatic bile in patients with extrahepatic bile duct obstruction.

Terada, Tadashi; Morita, T; Hoso, Masahiro; Nakanuma, Yasuni

doi:10.1136/jcp.47.10.924

Cited by 19 publications

(19 citation statements)

References 6 publications

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“…Western blot analysis showed specific signals of the pancreatic digestive enzymes in normal pancreas, thus confirming the specificity of the antibodies. The antibody against pancreatic lipase recognizes a 52-kDa protein [33], suggesting that the antibody reacts with pancreatic lipase but not with other lipases of different molecular weight. The antibody to pancreatic α-amylase does not react with salivary α-amylase [32].…”

Section: Discussionmentioning

confidence: 96%

“…Activity of pancreatic α-amylase, lipase and chymotrypsin was assayed according to the method of our previous study [33]. Concentration of trypsinogen was measured by radioimmunoassay according to the method of our previous study [33]. Fisher's exact test was used for statistical analysis, with a significant level of P<0.05.…”

Section: Methodsmentioning

confidence: 99%

“…The homogenate was centrifuged at 20,000 rpm for 15 min, and the supernatant was collected. Activity of pancreatic α-amylase, lipase and chymotrypsin was assayed according to the method of our previous study [33]. Concentration of trypsinogen was measured by radioimmunoassay according to the method of our previous study [33].…”

Section: Methodsmentioning

confidence: 99%

“…Proteins were extracted from these specimens by homogenization with lysis buffer and centrifugation. Western blot procedure was performed as previously described [33]. Detection of signals was performed by enhanced chemiluminescent technique using ECL kit (Amersham, Bucks., UK).…”

Section: Methodsmentioning

confidence: 99%

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Expression of pancreatic digestive enzymes in normal and pathologic epithelial cells of the human gastrointestinal system

et al. 1997

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Pancreatic digestive enzymes have rarely been reported in human nonpancreatic organs. We examined their expression in the epithelial cells of the nonpancreatic gastrointestinal organs, looking for pancreatic alpha-amylase, trypsin, chymotrypsin and pancreatic lipase. Western blotting, enzyme assay and pancreatic alpha-amylase mRNA were also used in selected specimens. In normal tissues, immunoreactivity of one or more of these enzymes was frequently noted in cells of the salivary glands, stomach, duodenum, large pancreatic ducts, extrahepatic bile ducts and gall bladder. The epithelium of the normal oesophagus, small intestine and colon were consistently negative for these enzymes. In pathologic tissues, immunoreactivity for one or more enzymes was present in epithelial cells of pleomorphic adenomas of the salivary glands, oesophageal squamous cell carcinoma, gastric adenoma and adenocarcinoma, pancreatic adenocarcinoma, cholecystitis, adenocarcinoma of the gall bladder and extrahepatic bile duct, and colon adenoma and adenocarcinoma. Western blotting showed a specific band of each enzyme in some specimens of normal stomach. In situ hybridization for pancreatic alpha-amylase mRNA showed specific signals in the normal stomach, but not in the normal colon. Reverse transcriptase polymerase chain reaction analysis for pancreatic alpha-amylase mRNA revealed specific signals in the normal stomach. Enzyme assay revealed that the stomach and gall bladder showed these activities. The data suggest that pancreatic digestive enzymes are produced by several epithelial cell types of the nonpancreatic gastrointestinal organs, that the organs positive for pancreatic enzyme have a common cell lineage, and that neoplasms continue to express or neoexpress these enzymes after neoplastic transformation.

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Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Expression of pancreatic digestive enzymes in normal and pathologic epithelial cells of the human gastrointestinal system

et al. 1997

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“…For example, while many proteins are expressed in both small and large cholangiocytes, some proteins are expressed only in small cholangiocytes or vice versa. In humans, both small and large cholangiocytes express pancreatic lipase, pancreatic -amylase, trypsin (263,264), and the Cl − -HCO 3 − exchanger, AE2 (177,264). In contrast, only small cholangiocytes express blood group antigens (e.g., A and B) (214) and the antiapoptotic protein, Bcl-2 (53,55), while only large cholangiocytes express cytochrome P4502E1 (61,152).…”

Section: Biochemical and Functional Heterogeneity Of Cholangiocytesmentioning

confidence: 99%

Physiology of Cholangiocytes

Masyuk¹,

Masyuk²,

LaRusso³

2012

Physiology of the Gastrointestinal Tract

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Cholangiocytes are epithelial cells that line the intra-and extrahepatic ducts of the biliary tree. The main physiologic function of cholangiocytes is modification of hepatocyte-derived bile, an intricate process regulated by hormones, peptides, nucleotides, neurotransmitters, and other molecules through intracellular signaling pathways and cascades. The mechanisms and regulation of bile modification are reviewed herein.

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Physiology of Cholangiocytes

Tabibian

Masyuk

et al. 2013

Comprehensive Physiology

208

207

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Cholangiocytes are epithelial cells that line the intra- and extrahepatic ducts of the biliary tree. The main physiologic function of cholangiocytes is modification of hepatocyte-derived bile, an intricate process regulated by hormones, peptides, nucleotides, neurotransmitters, and other molecules through intracellular signaling pathways and cascades. The mechanisms and regulation of bile modification are reviewed herein.

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Pancreatic enzymes in the epithelium of intrahepatic large bile ducts and in hepatic bile in patients with extrahepatic bile duct obstruction.

Abstract: Conclusions-These results strongly suggest that biliary epithelia of larger intrahepatic ducts produce pancreatic a-amylase, lipase, and trypsin, and that these enzymes are secreted into the lumina of intrahepatic bile ducts.( 7 Clin Pathol 1994;47:924-927)

Cited by 19 publications

References 6 publications

Expression of pancreatic digestive enzymes in normal and pathologic epithelial cells of the human gastrointestinal system

Expression of pancreatic digestive enzymes in normal and pathologic epithelial cells of the human gastrointestinal system

Physiology of Cholangiocytes

Physiology of Cholangiocytes

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