Pancreatic islet banking: The transplantation of frozen-thawed rat islets transported between centers

Rajotte, Ray V.; Scharp, David W.; Downing, R.; Preston, Robert B.; Molnar, George D.; Ballinger, Walter F.; Greider, Marie H.

doi:10.1016/0011-2240(81)90108-5

Cited by 58 publications

(25 citation statements)

References 16 publications

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“…Should pancreatic islet transplantation become clini cally applicable, cryogenic storage of the iso lated islets will facilitate transplantation man agement [ 1 ]. In addition, the cryopreservation procedure may have the potential to overcome different problems related to clinical islet transplantation, i.e.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Islets of Langerhans Does Not Affect Angiogenesis and Revascularization after Free Transplantation

Menger¹,

Pattenier

Wolf

et al. 1992

Eur Surg Res

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Cryopreservation of isolated islets of Langerhans will be a necessary procedure if pancreatic islet transplantation crosses the threshold for clinical treatment of diabetes mellitus. Although successful cryopreservation of rodent, canine, porcine and human islets has been documented in the past few years, little is known about the influence of the freeze-thaw procedure on the islet’s potential to induce angiogenesis and revascularization, a process which is of crucial importance after free transplantation. We have analyzed the process of revascularîzation of 1- and 10-week-cryopreserved hamster islet isografts using intravital fluorescence microscopy. First signs of angiogenesis of cryopreserved islet grafts were observed on day 2 after transplantation, characterized by the protrusion of capillary sprouts. During the following days these sprouts formed a microvascular network, and revascularîzation was completed on day 10 after transplantation. Quantitative analysis of functional capillary density, capillary red blood cell velocity, capillary diameter and flow of individual capillaries did neither show differences between 1-and 10-week-cryopreserved islets, nor differences between cryopreserved islets and islets transplanted without cryopreservation were observed. From these results we conclude that cryopreservation of isolated pancreatic islet grafts is an adequate technique for long-term storage prior to transplantation.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of Islets of Langerhans Does Not Affect Angiogenesis and Revascularization after Free Transplantation

Menger¹,

Pattenier

Wolf

et al. 1992

Eur Surg Res

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“…We attempted to cryopreserve isolated chick islets following an established protocol used for cryopreservation of mouse islets [57]. The islets were cryopreserved in a medium consisting of DMEM:Ham's F.12 (1:1) (Gibco, Brazil), supplemented with 10% FCS, riboflavin, and nicotinamide as cryoprotectants [58][59][60][61][62][63][64]. We found that it is possible to cryopreserve chick islets without losing viability and insulin secretory activity [13].…”

Section: Usability Of Chick Pancreatic Islets To Screen For Insulin Smentioning

confidence: 99%

Modeling Chick to Assess Diabetes Pathogenesis and Treatment

Datar¹,

Bhonde²

2011

Rev Diabet Stud

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■ AbstractAnimal models have been used extensively in diabetes research. Studies on animal models have contributed to the discovery and purification of insulin, development of new therapeutic approaches, and progress in fundamental and clinical research. However, conventional rodent and large animal mammalian models face ethical, practical, or technical limitations. Therefore, it would be beneficial developing an alternative model for diabetes research which would overcome these limitations. Amongst other vertebrates, birds are phylogenically closer to mammals, and amongst birds, the chick has been used as one of the favored models in developmental biology, toxicology, cancer research, immunology, and drug testing. Chicken eggs are readily available, have a short incubation period and easily accessible embryos. Based on these inimitable advantages, the present review article aims to discuss the suitability of the chick as a model system to study specific aspects of diabetes. The review focuses on the application of i) chick pancreatic islets for screening of antidiabetic agents and for islet banking, (ii) shell-less chick embryo culture as a model to study hyperglycemia-induced malformations observed in mammalian embryos, and (iii) chick chorioallantoic membrane (CAM) to examine glucose-induced endothelial damage leading to inhibition of angiogenesis.

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“…Banks et al [272] found that freezing in 1 mol/1 DMSO at 75 ~ gave optimal viability by in vitro parameters. Rajotte et al [275] found different conditions to be optimal: stepwise suspension in 2 mol/1DMSO, seeding with ice at -7.5 ~ freezing to -75 ~ at 0.25 ~ min, storage at -196 ~ and warming at 7.5 ~ Bretzel et al [276] have also normalized blood glucose in diabetic rats with isologous islets frozen in 1 mol/1 DMSO at 2 ~ and stored for 4 weeks at -196 ~ before rapid thawing and intraportal transplantation.…”

Section: Cryopreservation Of Islet Tissue a Variety Of Cells Ormentioning

confidence: 99%

“…Payne et al [277] used a protocol similar to that of Rajotte et al [275] to freeze neonatal rat pancreatic fragments to --70 ~ Fragments were thawed at room temperature, dispersed by collagenase digestion and transplanted intraportally to syngeneic diabetic rats. Only half of the rats receiving no further treatment were cured of diabetes, but 12 of 13 rats treated with insulin for 1 to 2 weeks became permanently normoglycaemic.…”

Section: Cryopreservation Of Islet Tissue a Variety Of Cells Ormentioning

confidence: 99%

Pancreas and islet transplantation

Sutherland

1981

Diabetologia

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Pancreatic islet banking: The transplantation of frozen-thawed rat islets transported between centers

Cited by 58 publications

References 16 publications

Cryopreservation of Islets of Langerhans Does Not Affect Angiogenesis and Revascularization after Free Transplantation

Cryopreservation of Islets of Langerhans Does Not Affect Angiogenesis and Revascularization after Free Transplantation

Modeling Chick to Assess Diabetes Pathogenesis and Treatment

Pancreas and islet transplantation

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